Combined antitumor effects of P‑5m octapeptide and 5‑fluorouracil on a murine model of H22 hepatoma ascites

Han, Xiao; An, Lihui; Yan, Dongmei; Matsuura, Hiroshi; Ding, Wei‐Guang; Zhang, Mengchuan; Xu, Guangyu; Sun, Ying; Gao, Yuan; Wang, Manli; Zhao, Nanxi; Sun, Jingbo; Zhu, X. D.

doi:10.3892/etm.2018.6422

Cited by 2 publications

(3 citation statements)

References 27 publications

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“…In this study, the treatment with 5-FU was performed intraperitoneally [37,38,40]. Although 5-FU is administered intravenously in humans, some studies have shown that the effectiveness of 5-FU was not affected by the route of administration [40,41]. After administration, most of the 5-FU is immediately catabolized by the dihydropyrimidine dehydrogenase (DPD) enzyme.…”

Section: Discussionmentioning

confidence: 99%

“…Our results show that there was no lipid peroxidation in the kidneys due to increased SOD and CAT activity, and GSH intake. One way to avoid lipid peroxidation by an excess of ROS is to increase the activity of endogenous antioxidants, maintaining a redox balance [40]. SOD performs the dismutation of O 2 − to H 2 O 2, and CAT converts this compound into H 2 O and O 2 or is eliminated by glutathione peroxidase using GSH via GST catalysis [68].…”

Section: Discussionmentioning

confidence: 99%

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A Rodent Model of Human-Dose-Equivalent 5-Fluorouracil: Toxicity in the Liver, Kidneys, and Lungs

et al. 2023

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5-Fluorouracil (5-FU) is a chemotherapy drug widely used to treat a range of cancer types, despite the recurrence of adverse reactions. Therefore, information on its side effects when administered at a clinically recommended dose is relevant. On this basis, we examined the effects of the 5-FU clinical treatment on the integrity of the liver, kidneys, and lungs of rats. For this purpose, 14 male Wistar rats were divided into treated and control groups and 5-FU was administered at 15 mg/kg (4 consecutive days), 6 mg/kg (4 alternate days), and 15 mg/kg on the 14th day. On the 15th day, blood, liver, kidney, and lung samples were collected for histological, oxidative stress, and inflammatory evaluations. We observed a reduction in the antioxidant markers and an increase in lipid hydroperoxides (LOOH) in the liver of treated animals. We also detected elevated levels of inflammatory markers, histological lesions, apoptotic cells, and aspartate aminotransferase. Clinical treatment with 5-FU did not promote inflammatory or oxidative alterations in the kidney samples; however, histological and biochemical changes were observed, including increased serum urea and uric acid. 5-FU reduces endogenous antioxidant defenses and increases LOOH levels in the lungs, suggesting oxidative stress. Inflammation and histopathological alterations were also detected. The clinical protocol of 5-FU promotes toxicity in the liver, kidneys, and lungs of healthy rats, resulting in different levels of histological and biochemical alterations. These results will be useful in the search for new adjuvants to attenuate the adverse effects of 5-FU in such organs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Rodent Model of Human-Dose-Equivalent 5-Fluorouracil: Toxicity in the Liver, Kidneys, and Lungs

et al. 2023

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“…for four cycles every five days (on the 12th, 17th, 22nd, and 27th days). Group (V): SEC bearing mice + 5-FU (20 mg/kg) [67] injected i.p. on alternate days from the 12th day till the end of the experiment (n = 15).…”

Section: Effect Of Short-term Storagementioning

confidence: 99%

Metformin Loaded Zein Polymeric Nanoparticles to Augment Antitumor Activity against Ehrlich Carcinoma via Activation of AMPK Pathway: D-Optimal Design Optimization, In Vitro Characterization, and In Vivo Study

Elmahboub,

Albash,

Magdy William

et al. 2024

Molecules

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Metformin (MET), an antidiabetic drug, is emerging as a promising anticancer agent. This study was initiated to investigate the antitumor effects and potential molecular targets of MET in mice bearing solid Ehrlich carcinoma (SEC) as a model of breast cancer (BC) and to explore the potential of zein nanoparticles (ZNs) as a carrier for improving the anticancer effect of MET. ZNs were fabricated through ethanol injection followed by probe sonication method. The optimum ZN formulation (ZN8) was spherical and contained 5 mg zein and 30 mg sodium deoxycholate with a small particle size and high entrapment efficiency percentage and zeta potential. A stability study showed that ZN8 was stable for up to three months. In vitro release profiles proved the sustained effect of ZN8 compared to the MET solution. Treatment of SEC-bearing mice with ZN8 produced a more pronounced anticancer effect which was mediated by upregulation of P53 and miRNA-543 as well as downregulation of NF-κB and miRNA-191-5p gene expression. Furthermore, ZN8 produced a marked elevation in pAMPK and caspase-3 levels as well as a significant decrease in cyclin D1, COX-2, and PGE2 levels. The acquired findings verified the potency of MET-loaded ZNs as a treatment approach for BC.

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Combined antitumor effects of P‑5m octapeptide and 5‑fluorouracil on a murine model of H22 hepatoma ascites

Cited by 2 publications

References 27 publications

A Rodent Model of Human-Dose-Equivalent 5-Fluorouracil: Toxicity in the Liver, Kidneys, and Lungs

A Rodent Model of Human-Dose-Equivalent 5-Fluorouracil: Toxicity in the Liver, Kidneys, and Lungs

Metformin Loaded Zein Polymeric Nanoparticles to Augment Antitumor Activity against Ehrlich Carcinoma via Activation of AMPK Pathway: D-Optimal Design Optimization, In Vitro Characterization, and In Vivo Study

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