Combined analysis of T cell activation and T cell-mediated cytotoxicity by imaging cytometry

Chanda, Monica; Shudde, Claire E; Piper, Taylor L; Zheng, Yating; Courtney, Adam H.

doi:10.1016/j.jim.2022.113290

Cited by 1 publication

(1 citation statement)

References 47 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We highlight five clusters which likely serve functionally distinct roles in homeostasis and regeneration: progenitor (GLI1+ and CD74+), pre-adipogenic (DLK1+), pre-fibrogenic (CD55+), and transitional mechanosensitive (PIEZO2+) subpopulations. Utilizing full spectrum flow cytometry, we were able to resolve these unique stem and progenitor cell populations through surface markers and provide a roadmap for prospective isolation as full spectrum flow sorting machines become more widely available 86 . We then show that FAP subpopulations are perturbed in injury and these changes are highly sensitive to injury severity.…”

Section: Discussionmentioning

confidence: 99%

Distinct human stem cell subpopulations drive adipogenesis and fibrosis in musculoskeletal injury

Garcia,

Lau,

Diaz

et al. 2023

Preprint

View full text Add to dashboard Cite

Fibroadipogenic progenitors (FAPs) maintain healthy skeletal muscle in homeostasis but drive muscle degeneration in chronic injuries by promoting adipogenesis and fibrosis. To uncover how these stem cells switch from a pro-regenerative to pro-degenerative role we perform single-cell mRNA sequencing of human FAPs from healthy and injured muscles across a spectrum of injury. We identify multiple subpopulations with progenitor, adipogenic, or fibrogenic gene signatures. We utilize full spectrum flow cytometry to identify distinct FAP subpopulations based on highly multiplexed protein expression. We uncover that injury severity increases adipogenic commitment of FAP subpopulations and is driven by the downregulation of DLK1. Treatment of FAPs with DLK1 reduces adipogenesis, suggesting that during injury, reduced DLK1 within a subpopulation of FAPs may drive adipogenic degeneration. This work highlights how stem cells perform varied functions depending on tissue context, by dynamically regulating subpopulation fate commitment, which can be targeted improve patient outcomes after injury.

show abstract

Section: Discussionmentioning

confidence: 99%