Combined analysis of intracellular signalling and immunophenotype of human peripheral blood basophils by flow cytometry: a proof of concept

Ebo, Didier G.; Dombrecht, Evelyne; Bridts, Chris H.; Aerts, Nicolaas E.; Clerck, Luc S. De; Stevens, W. J.

doi:10.1111/j.1365-2222.2007.02819.x

Cited by 48 publications

(60 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, as expected, this was not associated with degranulation of the cells (as assessed by the upregulation of CD63) (29,30).…”

Section: Discussionmentioning

confidence: 73%

“…Although IL-3 was confirmed not to upregulate CD63 (29,30) and allergen did not trigger STAT5 phosphorylation, the observation that a mixture of IL-3 and allergen significantly augmented both phosphorylation of STAT5 and CD63 expression endorses the idea of a cross-talk between these two distinct signaling pathways. This is further illustrated by the inhibition experiments as only a mixture of WP1066 and Piceatannol was able to reduce STAT5 phosphorylation.…”

Section: Discussionmentioning

confidence: 87%

See 1 more Smart Citation

STAT5 in human basophils: IL‐3 is required for its FcεRI‐mediated phosphorylation

Verweij

Sabato

Nullens

et al. 2011

Cytometry Part B Clinical

View full text Add to dashboard Cite

“…However, as expected, this was not associated with degranulation of the cells (as assessed by the upregulation of CD63) (29,30).…”

Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 87%

STAT5 in human basophils: IL‐3 is required for its FcεRI‐mediated phosphorylation

Verweij

Sabato

Nullens

et al. 2011

Cytometry Part B Clinical

View full text Add to dashboard Cite

“…In this context, it is anticipated that the recently developed technique to analyze histamine by flow cytometry in combination with surface markers on a single cell level (4) might disclose data that are inaccessible to traditional histamine release assays in which results only represent an average from all stimulated cells. Moreover, combining the flowassisted analyses of phenotypic alterations and histamine release with staining of intracellular signal molecules (4,26,27) might further elucidate on the principles that govern basophil activation.…”

Section: Discussionmentioning

confidence: 99%

CD300a is expressed on human basophils and seems to inhibit IgE/FcεRI‐dependent anaphylactic degranulation

Sabato

Verweij

Bridts

et al. 2011

Cytometry Part B Clinical

View full text Add to dashboard Cite

Background. The final response that leads to basophil degranulation results from a cross-talk between activatory and inhibitory signals. However, in the context of basophil biology, the inhibitory mechanisms that control these processes are still poorly understood.Aim. To investigate the expression and function of the inhibitory receptor CD300a in human basophils. Methods. Peripheral blood of 20 patients with birch pollen allergy and 10 healthy control individuals were assessed for CD300a expression before and after activation. To study the function of CD300a, basophils were pre-incubated with anti-human CD300a/c monoclonal antibodies and analyzed for the upregulation of the activation marker CD203c and appearance of the degranulation marker CD63 following IgE-dependent and IgE-independent triggering.Results. Basophils from allergic individuals constitutively expressed significantly less CD300a than non-allergics (P 5 0.001). However, after cross-linking with either anti-IgE or recombinant major birch pollen allergen (rBet v 1), basophils from allergic patients demonstrated a significant and rapid (3 min) up-regulation of CD300a expression in all activated basophils that persisted for over 2 h (P < 0.05). Moreover, CD300a expression was significantly higher in the CD203c bright1 CD63 bright1 subpopulation than in CD203c bright1 CD63 2 cells (P < 0.05). In both patients and controls, pre-incubation with antiCD300a/c significantly inhibited IgE-mediated CD63 expression (P < 0.05), though it did not affect CD203c. In contrast, IgE-independent basophil activation was not inhibited by CD300a/c engagement.Conclusion. CD300a is expressed on human peripheral blood basophils and rapidly up-regulated upon cross-linking of IgE/FceRI and suppresses anaphylactic degranulation. V C 2011 International Clinical Cytometry Society

show abstract

“…The basophil activation test, based on flow cytometry, consists of analysing and quantifying changes in the expression of blood basophil activation markers in the presence of a given allergen (10,11,98). The most common activation markers are CD63 and CD203c.…”

Section: Delayed Allergic Hypersensitivitymentioning

confidence: 99%

Allergic hypersensitivity to topical and systemic corticosteroids: a review

Baeck

Marot

Nicolas

et al. 2009

Allergy

101

View full text Add to dashboard Cite

Corticosteroids, which are potent anti‐inflammatory and immunomodulator agents used in the treatment of various inflammatory diseases including allergic diseases, can in some cases produce immediate or delayed hypersensitivity reactions. This review summarizes the epidemiological and clinical characteristics of such reactions, including related diagnostic issues. It also presents a detailed analysis of the proposed immunological mechanisms including underlying cross‐reactions.

show abstract

Combined analysis of intracellular signalling and immunophenotype of human peripheral blood basophils by flow cytometry: a proof of concept

Cited by 48 publications

References 22 publications

STAT5 in human basophils: IL‐3 is required for its FcεRI‐mediated phosphorylation

STAT5 in human basophils: IL‐3 is required for its FcεRI‐mediated phosphorylation

CD300a is expressed on human basophils and seems to inhibit IgE/FcεRI‐dependent anaphylactic degranulation

Allergic hypersensitivity to topical and systemic corticosteroids: a review

Contact Info

Product

Resources

About