Combined administration of exogenous melatonin and insulin ameliorates streptozotocin induced toxic alteration on hematological parameters in diabetic male Wistar rats

Hajam, Younis Ahmad; Rai, Seema; Ghosh, Hindole; Basheer, Muddasir

doi:10.1016/j.toxrep.2020.01.020

Cited by 17 publications

(15 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Treatment with MLT significantly ameliorated hyperglycemia and the reduction of insulin levels, blocked the glycosylation of hemoglobin and improved insulin resistance in T2DM rats. These results corroborate the improvement of hyperglycemia by MLT treatment in several experimental models [ 3 , 34 , 35 ]. These findings agree with the ability of MLT to reverse the reduction of GLUT4 gene expression, glucose intolerance and insulin resistance, and augmented gluconeogenesis in MLT-deficient pinealectomized rats [ 36 , 37 ].…”

Section: Discussionsupporting

confidence: 88%

Melatonin protects the heart and pancreas by improving glucose homeostasis, oxidative stress, inflammation and apoptosis in T2DM-induced rats

Abdulwahab

El-Missiry

Shabana

et al. 2021

Heliyon

View full text Add to dashboard Cite

Cardiomyopathy and pancreatic injury are health issues associated with type 2 diabetes mellitus (T2DM) and are characterized by elevated oxidative stress, inflammation and apoptosis. Melatonin (MLT) is a hormone with multifunctional antioxidant activity. The protective effects of MLT on the heart and pancreas during the early development of diabetic cardiomyopathy and pancreatic injury were investigated in male Wistar rats with T2DM. MLT (10 mg/kg) was administered daily by gavage for 15 days after diabetic induction. Treatment of diabetic rats with MLT significantly normalized the levels of serum glucose, HbA1-c, and the lipid profile and improved the insulin levels and insulin resistance compared with diabetic rats, affirming its antidiabetic effect. MLT significantly prevented the development of oxidative stress and sustained the levels of glutathione and glutathione peroxidase activity in the heart and pancreas of diabetic animals, indicating its antioxidant capacity. Additionally, MLT prevented the increase in proinflammatory cytokines and expression of Bax, caspase-3 and P53. Furthermore, MLT enhanced the anti-inflammatory cytokine IL-10 and antiapoptotic protein Bcl-2. MLT controlled the levels of troponin T and creatine kinase-MB and lactate dehydrogenase activity, indicating its anti-inflammatory and antiapoptotic effects. Histological examinations confirmed the protective effects of MLT on T2DM-induced injury in the myocardium, pancreas and islets of Langerhans. In conclusion, the protective effects of melatonin on the heart and pancreas during the early development of T2DM are attributed to its antihyperglycemic, antilipidemic and antioxidant influences as well as its remarkable anti-inflammatory and antiapoptotic properties.

show abstract

Section: Discussionsupporting

confidence: 88%

Melatonin protects the heart and pancreas by improving glucose homeostasis, oxidative stress, inflammation and apoptosis in T2DM-induced rats

Abdulwahab

El-Missiry

Shabana

et al. 2021

Heliyon

View full text Add to dashboard Cite

show abstract

“…In the present study, the diabetic status did not alter the number of leukocytes (Table 1); but this change can occur and is typical of the damage caused by induced diabetes, as reported by 46 . The other parameters (Hem and Hb) also showed no statistical change.…”

Section: Discussionsupporting

confidence: 74%

“…The other parameters (Hem and Hb) also showed no statistical change. Hyperglycemia can modify red blood cell count and cause oxidative stress, hemoglobin glycation and other hematological disorders 46 . Studies have shown the ability of phenolic compounds to form complexes with Fe (II), present in large quantities in the hemoglobin molecule, which could interfere with its absorption in the gastrointestinal tract and consequently lead to iron deficiency [47][48][49] .…”

Section: Discussionmentioning

confidence: 99%

Coffee intake (Coffea arabica L.) reduces advanced glycation end product (AGEs) formation and platelet aggregation in diabetic rats

Silvério

Pereira

Duarte

et al. 2021

Rev. Ciênc. Farm. Básica Apl.

View full text Add to dashboard Cite

Objectives:The study aimed to determine the effect of coffee intake on AGEs formation and platelet aggregation in diabetic Wistar rats. Methods: Coffee powder samples were used to prepare a 10% beverage. Diabetes mellitus was induced in the animals by administering 2% alloxan. All animal experiments were approved by the ethics committee for animal experiments under N°. 420/2012 and 536/2013. Diabetic and non-diabetic rats were divided into 6 groups treated and untreated with coffee (7.2 mL/Kg body weight) and aminoguanidine (AGE inhibiting agent) (100 mg/Kg body weight) for 50 days. After 50 days, the animals were fasted for 12 h and anesthetized (40 mg/Kg sodium pentobarbital) intraperitoneally. Blood samples were collected from the abdominal artery puncture. Hematological parameters (red cells, hemoglobin, hematocrit and leukocyte) and glycemic and HbA1c levels were measured. AGEs quantification (spectrofluorometric method) and the platelet aggregation test (aggregation of cuvettes in a fourchannel platelet aggregometer) were also conducted. The rats' renal function was evaluated by measuring serum urea and creatinine. Results: Data showed that coffee intake had no effect on the hematological parameters. Fasting glucose and HbA1c dosage were significantly higher in diabetic animals compared to non-diabetic animals (confirmed the effectiveness of inducing and maintaining diabetic status). Results showed that coffee reduced AGE formation and platelet aggregation in our animal model, not altering the animals' renal function. Conclusions: These results suggest beneficial effects on vasculopathy, a common complication in diabetic patients.

show abstract

“…For example, unhealthy eating [ 2 ]. In patients with these pathologies, shorter telomeres were found in comparison with conventionally healthy study participants [ [43] , [44] , [45] , [46] ]. However, it has not yet been established what is the root cause of this pathological process: shortening of telomere length is due to a disease, or shorter telomeres contribute to the development of age-related diseases.…”

Section: Introductionmentioning

confidence: 99%

An original biomarker for the risk of developing cardiovascular diseases and their complications: Telomere length

et al. 2021

View full text Add to dashboard Cite

show abstract

Combined administration of exogenous melatonin and insulin ameliorates streptozotocin induced toxic alteration on hematological parameters in diabetic male Wistar rats

Cited by 17 publications

References 37 publications

Melatonin protects the heart and pancreas by improving glucose homeostasis, oxidative stress, inflammation and apoptosis in T2DM-induced rats

Melatonin protects the heart and pancreas by improving glucose homeostasis, oxidative stress, inflammation and apoptosis in T2DM-induced rats

Coffee intake (Coffea arabica L.) reduces advanced glycation end product (AGEs) formation and platelet aggregation in diabetic rats

An original biomarker for the risk of developing cardiovascular diseases and their complications: Telomere length

Contact Info

Product

Resources

About