Combined action of ribovirin and rimantadine in experimental myxovirus infection

Галегов, Г. А.; Pushkarskaya, N. L.; Obrosova-Serova, N. P.; Zhdanov, V. M.

doi:10.1007/bf01951273

Cited by 27 publications

(17 citation statements)

References 4 publications

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“…Previously, Galegov et al reported an increased antiviral effect with rimantadine hydrochloride and ribavirin in in vitro experiments with an avian influenza A virus and in mouse protection experiments with influenza A2/Frunze virus (7). The present study extended this observation to include contemporary human strains and multiple serotypes of influenza A viruses.…”

Section: Discussionsupporting

confidence: 72%

“…The inhibitory effects of single drugs or combinations were overcome at higher multiplicities of infection. An inoculum effect has been recognized previously in drug susceptibility tests with amantadine and rimantadine (7,9). Galegov et al found that the combination of rimantadine hydrochloride and ribavirin continued to demonstrate an enhanced antiviral effect despite progressively higher multiplicities of infection, although eightfold-higher ribavirin concentrations were used in their study than in the present one.…”

Section: Discussionmentioning

confidence: 76%

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Enhancement of activity against influenza viruses by combinations of antiviral agents

Hayden¹,

Douglas²,

Simons³

1980

Antimicrob Agents Chemother

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In an investigation of alternative therapeutic approaches for the treatment of influenza virus infections, the antiviral activities of rimantadine hydrochloride, amantadine hydrochloride, ribavirin, and combinations of these drugs were assessed in vitro. Madin-Darby canine kidney cell monolayers were inoculated with recent isolates of influenza viruses at low multiplicities of infection, and virus titers were determined after 24 h. The combination of rimantadine and ribavirin resulted in an enhanced antiviral effect (a decrease in virus titer of >1.0 logio plaque-forming unit per ml at 24 h relative to the maximal effect of a single drug) against A/USSR/90/77/H1N1, A/Texas/1/77/H3N2, A/New Jersey/76/ HSWlN1, and A/PR/834/HON1 viruses. The degree of inhibition depended on the virus strain used, the drug concentrations, and the virus inoculum. Amantadine and ribavirin showed enhanced activity. Ribavirin in combination with high (50 ,ug/ml), but not low (1.56 to 25 ,ug/ml), concentrations of rimantadine showed an enhanced antiviral effect against B/Hong Kong/72 virus. An assay of MadinDarby canine kidney cell proliferation in the presence of drugs showed that the enhanced inhibitory effect of drug combinations was not due to increased cytotoxicity.The adamantane compounds amantadine hydrochloride (Symmetrel) and rimantadine hydrochloride have well documented prophylactic and therapeutic activities against uncomplicated human influenza A virus infections (6, 17, 27; L.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 76%

Enhancement of activity against influenza viruses by combinations of antiviral agents

Hayden¹,

Douglas²,

Simons³

1980

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Before each experiment, thawed samples were further diluted in Eagle minimal essential medium containing 1% glutamine, 100 U of penicillin per ml and 50 ,ug of gentamicin per ml. (5,9,15).…”

Section: Methodsmentioning

confidence: 99%

Combined interferon-alpha 2, rimantadine hydrochloride, and ribavirin inhibition of influenza virus replication in vitro

Hayden

Schlepushkin

Pushkarskaya

1984

Antimicrob Agents Chemother

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Recombinant DNA-produced human interferon-alpha 2 inhibited the replication of influenza A and B viruses in primary rhesus monkey kidney cells (RMK). Human interferon-alpha 2 interacted additively or synergistically with rimantadine hydrochloride or ribavirin in reducing the yield of clinical isolates of either H3N2 or H1N1 subtype influenza A viruses. The combination of human interferon-alpha 2 and ribavirin also inhibited the replication of an influenza B virus to a greater extent than either single agent. In addition to drug concentration, the virus inoculum and duration of culture were important variables in determining the degree of inhibition. Single drugs or combinations did not significantly inhibit the growth of uninfected RMK cells, which indicated that the observed interactions with respect to antiviral activity were not due to cell cytotoxicity.

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“…Historically, the first in vitro investigations were carried out on the combination effect of the non-specific viral inhibitor ribavirin with amantadine against A (H7N7), (H3N2) and (H1N1) influenza virus in cell cultures (24,34,35) and against H3N2 in ferret tracheal ciliated epithelium (8). Further, ribavirin was tested in combination with zanamivir (the first NAI) and rimantadine interacting in an additive fashion against influenza A (H3N2) and (H1N1) virus in MDCK cells and with 2'-deoxy-2'-fluoroguanidine (2-FDG), a nucleoside analogue, it was synergistic against H1N1 subtype (50).…”

Section: Combined Therapy With Ribavirinmentioning

confidence: 99%

Chemotherapy of Influenza: Current and Novel Approach

Simeonova

Galabov

2011

Biotechnology & Biotechnological Equipment

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Combined action of ribovirin and rimantadine in experimental myxovirus infection

Cited by 27 publications

References 4 publications

Enhancement of activity against influenza viruses by combinations of antiviral agents

Enhancement of activity against influenza viruses by combinations of antiviral agents

Combined interferon-alpha 2, rimantadine hydrochloride, and ribavirin inhibition of influenza virus replication in vitro

Chemotherapy of Influenza: Current and Novel Approach

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