Combined 3D-spectrofluorometry, high performance liquid chromatography and capillary electrophoresis for the characterization of dissolved organic matter in natural waters

Parlanti, E.; Morin, Bénédicte; Vacher, Lilian

doi:10.1016/s0146-6380(01)00154-1

Cited by 73 publications

(58 citation statements)

References 24 publications

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“…Chin et al 1994, Peuravuori & Pihlaja 1997. For seawater, however, few attempts have been made (Parlanti et al 2002, Dittmer & Katter 2003, mainly due to the effect of sea salts on the elution pattern.…”

Section: Introductionmentioning

confidence: 99%

Bacterial production of marine humic-like fluorescent dissolved organic matter and its biogeochemical importance

Shimotori¹,

Omori²,

Hama³

2009

Aquat. Microb. Ecol.

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Bacterial production of humic-like fluorescent dissolved organic matter (FDOM H ) was examined in a 90 d culture experiment using a bacterial assemblage collected from coastal water with artificial seawater amended by glucose. A rapid decrease in dissolved organic carbon concentration was noticed during Days 1 to 5, and this coincided with an increase in bacterial numbers. The increase in FDOM H , which was determined by Excitation-Emission Matrix Spectroscopy (EEMS), was observed by Day 20, showing the production of FDOM H by bacteria. This increase lagged behind the increase in bacterial numbers, suggesting that the production of

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Section: Introductionmentioning

confidence: 99%

Bacterial production of marine humic-like fluorescent dissolved organic matter and its biogeochemical importance

Shimotori¹,

Omori²,

Hama³

2009

Aquat. Microb. Ecol.

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“…29 In order to present an in-depth analysis of the data and also to compare our findings to related studies, a few single-scan spectra are also included.…”

Section: -11mentioning

confidence: 99%

“…This technique has, since 1990, satisfactorily been used to investigate water masses in natural environments but reports on its utilization in NOM and HS fractionation studies have only recently appeared in the literature. [21][22][23][24][25][26][27][28][29][30] Briefly, Parlanti et al 29 have shown that the detailed off-line analysis of EEM fluorescence diagrams of NOM fractions allows the differentiation between marine and fresh water samples and Wu et al, 30 with on-line EEM fluorescence detection, found strong relationships between molecular size, fluorescence pattern and polarity for both NOM and HS, with the humic, fulvic and protein-like fluorescence fractions presenting distinct hydrophilic/hydrophobic natures.In this study, HS were fractionated, with a polymeric C 18 as the stationary phase, using a polarity gradient from pure water to pure acetonitrile, and a fluorescence detector. 29 In order to present an in-depth analysis of the data and also to compare our findings to related studies, a few single-scan spectra are also included.…”

mentioning

confidence: 99%

“…[21][22][23][24][25][26][27][28][29][30] Briefly, Parlanti et al 29 have shown that the detailed off-line analysis of EEM fluorescence diagrams of NOM fractions allows the differentiation between marine and fresh water samples and Wu et al, 30 with on-line EEM fluorescence detection, found strong relationships between molecular size, fluorescence pattern and polarity for both NOM and HS, with the humic, fulvic and protein-like fluorescence fractions presenting distinct hydrophilic/hydrophobic natures.…”

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confidence: 99%

“…Considering their overall shape these EEM are similar to those of natural, mainly fresh and pore, waters. 24,26,29,34 The fluorophores responsible for these two main signals have already been recognized as belonging to typical humiclike components, having received individual designations such as, A and C or, α´ and α, respectively. 24,26,29,34 Our observations extend only to λ exc = 250 nm and because of this, for the FA sample whose excitation ranges extend towards the blue-end, the α´ peak is only partially registered.…”

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3D-fluorescence spectroscopic analysis of HPLC fractionated estuarine fulvic and humic acids

Sierra¹,

Giovanela²,

Parlanti³

et al. 2006

J. Braz. Chem. Soc.

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Seis amostras de ácidos fúlvicos (AF) e húmicos (AH) estuarinos foram fracionadas por meio de cromatografia líquida de alta performance em fase reversa, num gradiente de polaridade água → acetonitrila, com detecção por fluorescência. Diferenças entre os cromatogramas mostraram que os AH contêm mais componentes hidrofóbicos que os AF. Os espectros de fluorescência tridimensional das frações foram bastante similares entre si e também ao da amostra original, indicando que, apesar das polaridades distintas, a complexidade e as principais características do material original se mantêm. Um mesmo grupo de fluoróforos parece ser responsável pelas características de fluorescência, dos dois tipos de substâncias húmicas. Um deslocamento batocrômico dos espectros de emissão das soluções originais, com relação àqueles das frações, foi atribuído a uma maior proximidade destes fluoróforos, possivelmente devido à formação de agregados. Em geral, os resultados confirmam o conceito de que as "macromoléculas" húmicas consistem de associações de estruturas menores com características químicas semelhantes.Six estuarine fulvic (FA) and humic acids (HA) were fractionated with reversed-phase high performance liquid chromatography in a water/acetonitrile gradient, with fluorescence detection. Differences between FA and HA chromatograms pointed to a higher incidence of hydrophobic components in the latter. Excitation-emission matrix fluorescence diagrams of most fractions were similar to those of the bulk samples indicating that, in spite of their distinct polarities, the complexity and main spectral characteristics of the materials persisted. A primary group of fluorophores, which are present in most of the fractions, seems to be at the origin of the fluorescence properties of both kinds of HS. A bathochromic shift of the bulk solutions spectra in relation to the fraction emissions was attributed to a greater proximity of these fluorophores, possibly due to the formation of aggregates. Generally, the results reinforced concepts that humic macromolecules consist of assemblages of structurally similar building blocks. Keywords: RP-HPLC, fulvic acids, humic acids, EEM fluorescence IntroductionHumic substances (HS) are complex macromolecular products resulting from the chemical and biological degradation of plant and animal residues. They are widely distributed in soils, natural waters, and sediments, and represent a significant proportion of the organic carbon in the global carbon cycle.1-4 According to current estimates, HS constitute the major fraction of soil organic matter (up to 80%) and the largest fraction of natural organic matter (NOM) in aquatic systems (up to 60% of dissolved organic carbon). 5,6These organic materials can have a substantial impact on a variety of biogeochemical processes, for example, immobilizing anthropogenic organic chemicals and heavy metals. 7-11The elucidation of the HS molecular sizes, shapes, weights and structural arrangements is essential for an [15][16][17][18][19] In general, UV absorbance, and/or ...

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EPR, FTIR, Raman, UV–Visible Absorption, and Fluorescence Spectroscopies in Studies of NOM

Martin‐Neto

Milori

Silva

et al. 2009

Biophysico‐Chemical Processes Involving Natural Nonliving Organic Matter in Environmental Systems

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Combined 3D-spectrofluorometry, high performance liquid chromatography and capillary electrophoresis for the characterization of dissolved organic matter in natural waters

Cited by 73 publications

References 24 publications

Bacterial production of marine humic-like fluorescent dissolved organic matter and its biogeochemical importance

Bacterial production of marine humic-like fluorescent dissolved organic matter and its biogeochemical importance

3D-fluorescence spectroscopic analysis of HPLC fractionated estuarine fulvic and humic acids

EPR, FTIR, Raman, UV–Visible Absorption, and Fluorescence Spectroscopies in Studies of NOM

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