Combinatorial optimization of cyanobacterial 2,3-butanediol production

Oliver, John W. K.; Machado, Iara; Yoneda, Hisanari; Atsumi, Shota

doi:10.1016/j.ymben.2014.01.001

Cited by 100 publications

(62 citation statements)

References 37 publications

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“…The productivity of 3HP demonstrated in this study exceeds or compares favorably to those of the other hydroxyacids demonstrated. Furthermore, among the various products produced using engineered cyanobacteria, only a few cases were able to achieve production titer over 500 mg/L(Oliver and Atsumi, 2014). The production titers achieved in this study is relatively high with observed titer reaching 665 mg/L from the best producing strain developed in this study.…”

mentioning

confidence: 69%

Metabolic engineering of cyanobacteria for photosynthetic 3-hydroxypropionic acid production from CO2 using Synechococcus elongatus PCC 7942

Lan

Chuang

Shen

et al. 2015

Metabolic Engineering

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Photosynthetic conversion of CO2 to chemicals using cyanobacteria is an attractive approach for direct recycling of CO2 to useful products. 3-Hydroxypropionic acid (3 HP) is a valuable chemical for the synthesis of polymers and serves as a precursor to many other chemicals such as acrylic acid. 3 HP is naturally produced through glycerol metabolism. However, cyanobacteria do not possess pathways for synthesizing glycerol and converting glycerol to 3 HP. Furthermore, the latter pathway requires coenzyme B12, or an oxygen sensitive, coenzyme B12-independent enzyme. These characteristics present major challenges for production of 3 HP using cyanobacteria. To overcome such difficulties, we constructed two alternative pathways in Synechococcus elongatus PCC 7942: a malonyl-CoA dependent pathway and a β-alanine dependent pathway. Expression of the malonyl-CoA dependent pathway genes (malonyl-CoA reductase and malonate semialdehyde reductase) enabled S. elongatus to synthesize 3 HP to a final titer of 665 mg/L. β-Alanine dependent pathway expressing S. elongatus produced 3H P to final titer of 186 mg/L. These results demonstrated the feasibility of converting CO2 into 3 HP using cyanobacteria.

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mentioning

confidence: 69%

Metabolic engineering of cyanobacteria for photosynthetic 3-hydroxypropionic acid production from CO2 using Synechococcus elongatus PCC 7942

Lan

Chuang

Shen

et al. 2015

Metabolic Engineering

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“…Often, engineered pathways have been optimized through sampling of large design spaces (Pfleger et al 2006; Lütke-Ever- Wang et al 2012a;Xu et al 2013;Nowroozi et al 2014). Such combinatorial approaches are specifically valuable for organisms with less-defined genetic and metabolic networks (Oliver et al 2014). However, combinatorial libraries are limited by analytical capabilities and require high-throughput screens or selection assays.…”

Section: Balanced Pathway Fluxesmentioning

confidence: 99%

The Need for Integrated Approaches in Metabolic Engineering

Lechner

Brunk

Keasling

2016

Cold Spring Harb Perspect Biol

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This review highlights state-of-the-art procedures for heterologous small-molecule biosynthesis, the associated bottlenecks, and new strategies that have the potential to accelerate future accomplishments in metabolic engineering. We emphasize that a combination of different approaches over multiple time and size scales must be considered for successful pathway engineering in a heterologous host. We have classified these optimization procedures based on the "system" that is being manipulated: transcriptome, translatome, proteome, or reactome. By bridging multiple disciplines, including molecular biology, biochemistry, biophysics, and computational sciences, we can create an integral framework for the discovery and implementation of novel biosynthetic production routes. CURRENT APPROACHES IN METABOLIC ENGINEERING AND THEIR LIMITATIONS

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“…The simpler structure of cyanobacteria allows them to be more efficient at carbon fixation from the atmosphere than eukaryotic algae [41]. However, they yield a much smaller photosynthetic conversion efficiency compared to algae [42].…”

Section: Microalgae-based Technologies To Reduce Carbon Footprintmentioning

confidence: 99%

“…Moreover, several model cyanobacterial species have their genomes fully sequenced. The latter, combined with the fact that genetic manipulation of cyanobacteria is well established allowed for application of high throughput systems biol ogy approaches to engineer cyanobacterial strains producing HVPs [41][42][43][44][45][46].…”

Section: Microalgae-based Technologies To Reduce Carbon Footprintmentioning

confidence: 99%

Oxygenic photosynthesis: translation to solar fuel technologies

Olmos

Kargul

2014

Acta Soc Bot Pol

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Mitigation of man-made climate change, rapid depletion of readily available fossil fuel reserves and facing the growing energy demand that faces mankind in the near future drive the rapid development of economically viable, renewable energy production technologies. It is very likely that greenhouse gas emissions will lead to the significant climate change over the next fifty years. World energy consumption has doubled over the last twenty-five years, and is expected to double again in the next quarter of the 21st century. Our biosphere is at the verge of a severe energy crisis that can no longer be overlooked. Solar radiation represents the most abundant source of clean, renewable energy that is readily available for conversion to solar fuels. Developing clean technologies that utilize practically inexhaustible solar energy that reaches our planet and convert it into the high energy density solar fuels provides an attractive solution to resolving the global energy crisis that mankind faces in the not too distant future. Nature's oxygenic photosynthesis is the most fundamental process that has sustained life on Earth for more than 3.5 billion years through conversion of solar energy into energy of chemical bonds captured in biomass, food and fossil fuels. It is this process that has led to evolution of various forms of life as we know them today. Recent advances in imitating the natural process of photosynthesis by developing biohybrid and synthetic "artificial leaves" capable of solar energy conversion into clean fuels and other high value products, as well as advances in the mechanistic and structural aspects of the natural solar energy converters, photosystem I and photosystem II, allow to address the main challenges: how to maximize solar-to-fuel conversion efficiency, and most importantly: how to store the energy efficiently and use it without significant losses. Last but not least, the question of how to make the process of solar energy conversion into fuel not only efficient but also cost effective, therefore attractive to the consumer, should be properly addressed.

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Combinatorial optimization of cyanobacterial 2,3-butanediol production

Cited by 100 publications

References 37 publications

Metabolic engineering of cyanobacteria for photosynthetic 3-hydroxypropionic acid production from CO2 using Synechococcus elongatus PCC 7942

Metabolic engineering of cyanobacteria for photosynthetic 3-hydroxypropionic acid production from CO2 using Synechococcus elongatus PCC 7942

The Need for Integrated Approaches in Metabolic Engineering

Oxygenic photosynthesis: translation to solar fuel technologies

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