Combinatorial matrix-assisted pulsed laser evaporation: Single-step synthesis of biopolymer compositional gradient thin film assemblies

Abstract

Electrostatic quadrupole plasma mass spectrometer measurements during thin film depositions using simultaneous matrix assisted pulsed laser evaporation and magnetron sputtering

Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

“…In Figure 8.A are presented optical and fluorescent pair images in which one can observe the increasing of fluorescence intensity from OL to L along the deposited sample ( Figure 8.A and 8.C). This confirms the compositional gradient in the structure [13].…”

“…Combinatorial-MAPLE technique was introduced as a new approach for the fabrication of gradient organic/inorganic thin films, for the identification of best bioactive surfaces able to modulate and control cell behavior [13]. In C-MAPLE experiments, two targets are simultaneously evaporated by laser beams.…”

“…The new processing method called Combinatorial-MAPLE (C-MAPLE) was proposed to biopolymer compounds [13,14,22]. The composition gradient between two materials is achieved by laser co-evaporation of two distinct cryogenic targets and thin-film co-deposition process on solid substrate as described in Section 3.2.…”

“…Interestingly, the cell density on OL areas was superior to standard cover slips. It was suggested that such compositional gradients could be used to screen specific nanostructured surface cues for tailoring cell proliferation or to modulate intracellular signaling pathways for specific biomedical applications [13,14].…”

“…A representative example is for Levan (L) and oxidized Levan (OL) biopolymers, in vitro cell culture assays illustrating characteristic responses of cells to specific surface regions [13]. The versatility of the method for the synthesis of hybrid materials and further possible developments are summarized.…”