Combinatorial Laser Synthesis of Biomaterial Thin Films: Selection and Processing for Medical Applications

Axente, E.; Ristoscu, Carmen; Bigi, Adriana; Sima, Félix; Mihăilescu, I. N.

doi:10.1007/978-3-319-96845-2_11

Cited by 7 publications

(8 citation statements)

References 117 publications

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“…Detailed protocols for thin coating assembling by MAPLE were addressed elsewhere (Sima et al, 2016;Axente et al, 2018;Axente and Sima, 2020) and briefly described in the following. The procedure consists of laser irradiation of a cryogenic target by a pulsed UV laser beam (Figure 1).…”

Section: Maple Experimentsmentioning

confidence: 99%

“…Matrix Assisted Pulsed Laser Evaporation (MAPLE) is an additive physical vapor deposition technique developed for functionalization of solid substrates with protein and composite coatings. When compared to other classical deposition methods (e.g., drop-cast, spin-coating, dip-coating, Langmuir-Blodgett), this method provides high experimental versatility and relatively facile control of coating thickness even for ultrathin films, the possibility to preserve materials properties even for very delicate compounds, providing adherent and uniform coatings on centimeter sized substrates, micro-fabrication of multilayers from multi-target system, and possibility to generate gradient coatings in a single-step process (Sima et al, 2016;Axente et al, 2018;Axente and Sima, 2020). However, current drawbacks are related to difficulties to fabricate large-area coatings (tens of centimeters) with high uniformity.…”

Section: Introductionmentioning

confidence: 99%

“…Laser-assisted synthesis of thin organic coatings of highmolecular mass such as biopolymers, proteins, and enzymes present various technical challenges. In our previous studies (Sima et al, 2016;Axente et al, 2018;Axente and Sima, 2020) we reported synthesis of highly-functional organic, inorganic and hybrid coatings, for biomedical applications. MAPLE technique proved adequate for the delicate laser transfer of biopolymers, as e.g., levan, a high molecular weight, watersoluble bacterial exopolysaccharide (β2,6-linked fructan).…”

Section: Introductionmentioning

confidence: 99%

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Functionalized Graphene Oxide Thin Films for Anti-tumor Drug Delivery to Melanoma Cells

Sima

Chirițoiu²,

Neguț³

et al. 2020

Front. Chem.

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Since Graphene discovery, their associated derivate nanomaterials, Graphene Oxide (GO) and reduced-GO were in the forefront of continuous developments in bio-nano-technology due to unique physical-chemical properties. Although GO nano-colloids (GON) were proposed as drug release matrix for targeting cancer cells, there is still a concern regarding its cytotoxicity issues. In this study, we report on the fabrication of functional GON bio-coatings by Matrix-Assisted Pulsed Laser Evaporation (MAPLE) to be used as drug carriers for targeting melanoma cells. We first performed a thorough in vitro cytotoxicity assay for comparison between GON and protein functionalized GON coatings. As functionalization protein, Bovine Serum Albumin (BSA) was non-covalently conjugated to GO surface. Safe concentration windows were identified in cytotoxicity tests by live/dead staining and MTS assays for five different human melanoma cell lines as well as for non-transformed melanocytes and human dermal fibroblasts. Hybrid GON-BSA nano-scaled thin coatings incorporating Dabrafenib (DAB) and Trichostatin A (TSA) inhibitors for cells bearing BRAF V600E pathway activating mutation were assembled on solid substrates by MAPLE technique. We further demonstrated the successful immobilization for each drug-containing GON-BSA assembling systems by evaluating cellular BRAF activity inhibition and histone deacetylases activity blocking, respectively. DAB activity was proven by the decreased ERK phosphorylation in primary melanoma cells (SKmel28 BRAF V600E cell line), while TSA effect was evidenced by acetylated histones accumulation in cell's nuclei (SKmel23 BRAF WT cell line). In addition, melanoma cells exposed to GON-BSA coatings with compositional gradient of inhibitors evidenced a dose-dependent effect on target activity. Such functional bio-platforms could present high potential for cell-biomaterial interface engineering to be applied in personalized cancer therapy studies.

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Section: Maple Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Functionalized Graphene Oxide Thin Films for Anti-tumor Drug Delivery to Melanoma Cells

Sima

Chirițoiu²,

Neguț³

et al. 2020

Front. Chem.

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“…Inorganic, organic, hybrid coatings: Chitosan/ bio-mimetic apatite [61], CaPs, biopolymers [62,63] Levan and Chitosan blends [64], Graphene Oxide, BSA protein, drugs [65]/Implant devices, drug delivery, model surfaces.…”

Section: Combinatorial-matrix Assisted Pulsed Laser Evaporation (C-ma...mentioning

confidence: 99%

“…By readapting setup configurations, in particular employing multiple pulsed laser beams and/or multi-targets, combinatorial-PLD and combinatorial-MAPLE were developed to fabricate thin blended coatings with a gradient of composition in a single-step process. The key benefits and drawbacks of these techniques have been previously addressed in a comparison with other non-laser-based methods [7,62,63,97] and are briefly summarized in the following. Both methods are highly versatile, with individual process parameters able to control and tailor morphological and structural coating properties.…”

Section: Biomimetic Laser Processingmentioning

confidence: 99%

Biomimetic Coatings Obtained by Combinatorial Laser Technologies

Axente

Sima

2020

Coatings

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The modification of implant devices with biocompatible coatings has become necessary as a consequence of premature loosening of prosthesis. This is caused mainly by chronic inflammation or allergies that are triggered by implant wear, production of abrasion particles, and/or release of metallic ions from the implantable device surface. Specific to the implant tissue destination, it could require coatings with specific features in order to provide optimal osseointegration. Pulsed laser deposition (PLD) became a well-known physical vapor deposition technology that has been successfully applied to a large variety of biocompatible inorganic coatings for biomedical prosthetic applications. Matrix assisted pulsed laser evaporation (MAPLE) is a PLD-derived technology used for depositions of thin organic material coatings. In an attempt to surpass solvent related difficulties, when different solvents are used for blending various organic materials, combinatorial MAPLE was proposed to grow thin hybrid coatings, assembled in a gradient of composition. We review herein the evolution of the laser technological process and capabilities of growing thin bio-coatings with emphasis on blended or multilayered biomimetic combinations. These can be used either as implant surfaces with enhanced bioactivity for accelerating orthopedic integration and tissue regeneration or combinatorial bio-platforms for cancer research.

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