Combinatorial growth of oxide nanoscaffolds and its influence in osteoblast cell adhesion

Abstract

We report a novel method for high-throughput investigations on cell-material interactions based on metal oxide nanoscaffolds. These scaffolds possess a continuous gradient of various titanium alloys allowing the compositional and morphological variation that could substantially improve the formation of an osseointegrative interface with bone. The model nanoscaffold has been fabricated on commercially pure titanium (cp-Ti) substrate with a compositional gradients of tin (Sn), chromium (Cr), and niobium (Nb) deposited using a combinatorial approach followed by annealing to create native oxide surface. As an in vitro test system, the human fetal osteoblastic cell line (hFOB 1.19) has been used. Cell-adhesion of hFOB 1.19 cells and the suitability of these alloys have been evaluated for cell-morphology, cell-number, and protein adsorption. Although, cell-morphology was not affected by surface composition, cell-proliferation rates varied significantly with surface metal oxide composition; with the Sn-and Nb-rich regions showing the highest proliferation rate and the Cr-rich regions presenting the lowest. The results suggest that Sn and Nb rich regions on surface seems to promote hFOB 1.19 cell proliferation and may therefore be considered as implant material candidates that deserve further analysis. V C 2012 American Institute of Physics.

Electrospun Polyhydroxybutyrate/Poly(ε-caprolactone)/58S Sol–Gel Bioactive Glass Hybrid Scaffolds with Highly Improved Osteogenic Potential for Bone Tissue Engineering

“…Stellate cells can firmly attach, spread and eventually proliferate well on the matrix, whereas bipolar spindle cells are still migrating to locate sites for subsequent spreading and proliferation. 55 Thus, it is reasonable to conclude that the addition of silicate sol could promote the proliferation of MG-63 cells. The cell morphology changes and improved proliferation capacity can be mainly ascribed to two reasons: the calcium-dependent regulation and the highly enhanced hydrophilicity provided by the presence of the inorganic phase.…”

“…However, on day 7, more cells were found on P5B1 scaffold and the majority of cells on P5B1 presented multipolar polygonal morphologies (stellate) with short filopodia, whereas cells on P1B0 still showed bipolar spindle morphologies with relatively long filopodia. Stellate cells can firmly attach, spread and eventually proliferate well on the matrix, whereas bipolar spindle cells are still migrating to locate sites for subsequent spreading and proliferation . Thus, it is reasonable to conclude that the addition of silicate sol could promote the proliferation of MG-63 cells.…”

Advanced Biomaterials and 3D Printing Technologies in Bone Repair

“…These transmembrane proteins can interact with the ECM, directly or indirectly regulate the proliferation of stem cells, and promote cell adhesion and multidirectional differentiation (Abdal Dayem et al, 2018). Some related studies have shown that the micro-nanomorphology of the scaffold surface can affect the adsorption level of cells (Acevedo-Morantes et al, 2012;. Geng et al (2020a) simulated a continuous deep pit-like surface structure inside the gill cover of a snail on the surface of a titanium implant to increase the adsorption level of cells.…”

Study on the influence of scaffold morphology and structure on osteogenic performance

“…These transmembrane proteins can interact with the ECM, directly or indirectly regulate the proliferation of stem cells, and promote cell adhesion and multidirectional differentiation ( Abdal Dayem et al, 2018 ). Some related studies have shown that the micro-nanomorphology of the scaffold surface can affect the adsorption level of cells ( Acevedo-Morantes et al, 2012 ; Li H. et al, 2021 ). Geng et al (2020a) simulated a continuous deep pit-like surface structure inside the gill cover of a snail on the surface of a titanium implant to increase the adsorption level of cells.…”