Combinatorial effects of conception and governor vessel electroacupuncture and human umbilical cord blood-derived mesenchymal stem cells on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion

Yang, Zhuoxin; Pengdian, Chen; Yu, Haibo; Luo, Wenshu; Pi, Min; Wu, Yongdong; Yang, Fuxia; Gou, Yan-hua

doi:10.1016/s0254-6272(14)60012-1

Cited by 7 publications

(4 citation statements)

References 21 publications

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“…It has been shown that, transplantation of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) decreases the number of activated microglia and inhibits the permeation of immune cells and cellular apoptosis in the brain after ischemic brain injury (Yang et al., 2013; Zhu et al., 2014; Wang et al., 2016). Researchers have shown that the injection of hUCB-MSCs throughout the early stage of ischemic brain injury decreased the IL-1β, IL-6, and TNF-α expression levels in the serum and increased IL-10 expression levels.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Neuroprotective Properties ofMelissa officinalisin Combination With Human Umbilical Cord Blood Stem Cells After Spinal Cord Injury

et al. 2016

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IntroductionThe pathophysiology of spinal cord injury (SCI) has a classically bad prognosis. It has been demonstrated that human umbilical cord blood stem cells (hUCBSCs) and Melissa officinalis (MO) are useful for the prevention of neurological disease.MethodsThirty-six adult male rats were randomly divided into intact, sham, control (SCI), MO, hUCBSC, and MO-hUCBSC groups. Intraperitoneal injection of MO (150 mg/kg) was commenced 24 hr post-SCI and continued once a day for 14 days. Intraspinal grafting of hUCBSCs was commenced immediately in the next day. The motor and sensory functions of all animals were evaluated once a week after the commencement of SCI. Electromyography (EMG) was performed in the last day in order to measure the recruitment index. Immunohistochemistry, reverse transcription-polymerase chain reaction, and transmission electron microscopy evaluations were performed to determine the level of astrogliosis and myelination.ResultsThe results revealed that motor function (MO-hUCBSC: 15 ± 0.3, SCI: 8.2 ± 0.37, p < .001), sensory function (MO-hUCBSC: 3.57 ± 0.19, SCI: 6.38 ± 0.23, p < .001), and EMG recruitment index (MO-hUCBSC: 3.71 ± 0.18, SCI: 1.6 ± 0.1, p < .001) were significantly improved in the MO-hUCBSC group compared with SCI group. Mean cavity area (MO-hUCBSC: 0.03 ± 0.03, SCI: 0.07 ± 0.004, p < .001) was reduced and loss of lower motor neurons (MO-hUCBSC: 7.6 ± 0.43, SCI: 3 ± 0.12, p < .001) and astrogliosis density (MO-hUCBSC: 3.1 ± 0.15, SCI: 6.25 ± 1.42, p < 0.001) in the ventral horn of spinal cord were prevented in MO-hUCBSC group compared with SCI group.ConclusionThe results revealed that the combination of MO and hUCBSCs in comparison with the control group has neuroprotective effects in SCI.

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Section: Discussionmentioning

confidence: 99%

Assessment of Neuroprotective Properties ofMelissa officinalisin Combination With Human Umbilical Cord Blood Stem Cells After Spinal Cord Injury

et al. 2016

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“…For the detection of histopathological damage, sections were stained fixed in 10% formaldehyde and embedded in paraffin. The sections (5–6 µ m) were then stained with H&E for pathomorphological analysis, as described previously ( 20 ).…”

Section: Methodsmentioning

confidence: 99%

Growth differentiation factor 15 may protect the myocardium from no-reflow by inhibiting the inflammatory-like response that predominantly involves neutrophil infiltration

Zhang

Pan

Liu

et al. 2015

Molecular Medicine Reports

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The aim of the current study was to investigate the time course of the expression of growth differentiation factor-15 (GDF-15) in rat ischemic myocardium with increasing durations of reperfusion, and to elucidate its physiopathological role in the no-reflow phenomenon. Wistar rats were randomly divided into ischemia reperfusion (I/R) and sham groups, and myocardial I/R was established by ligation of the left anterior descending coronary artery for 1 h followed by reperfusion for 2, 4, 6, 12, 24 h and 7 days whilst rats in the sham group were subjected to a sham operation. The expression levels of GDF-15 and ICAM-1 were measured, in addition to myeloperoxidase (MPO) activity. The myocardial anatomical no-reflow and infarction areas were assessed. The area at risk was not significantly different following various periods of reperfusion, while the infarct area and no-reflow area were significantly greater following 6 h of reperfusion (P<0.05). The mRNA and protein expression levels of GDF-15 were increased during the onset and development of no-reflow, and peaked following 24 h of reperfusion. MPO activity was reduced with increasing reperfusion duration, while ICAM-1 levels were increased. Hematoxylin and eosin staining demonstrated that myocardial neutrophil infiltration was significantly increased by I/R injury, in particular following 2, 4 and 6 h of reperfusion. GDF-15 expression levels were negatively correlated with MPO activity (r=−0.55, P<0.001), and the MPO activity was negatively correlated with the area of no-reflow (r=−0.46, P<0.01). By contrast, GDF-15 was significantly positively correlated with ICAM-1 levels (r=0.52, P<0.01), which additionally were demonstrated to be significantly positively associated with the size of the no-reflow area (r= 0.39, P<0.05). The current study demonstrated the time course effect of reperfusion on the expression of GDF-15 in the myocardial I/R rat model, with the shorter reperfusion times (6 h) resulting in significant no-reflow in ischemic myocardium. GDF-15 may protect the I/R myocardium from no-reflow by inhibiting the inflammatory-like response, which involves neutrophil infiltration and transendothelial migration.

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“…Acupuncture of Sishencong [25] can nourish heart and tranquilize mind and soothe liver and relieve depression, thus improving the structure of sleep; acupuncture of Baihui [26] around which there are abundant blood vessels can treat insomnia. Modern studies have also confirmed that acupuncture can make patients in a state of qi-blood harmony, peaceful yin and compact yang in the treatment of insomnia [27,28] , thus improving the sleep quality of patients. Purpose of this paper is to collect high-quality clinical randomized controlled trials and strictly evaluate and analyze them one by one and then statistically process the data by quantitative synthesis and objectively evaluate them, so as to draw the comprehensive conclusion.…”

Section: Discussionmentioning

confidence: 99%

Meta-Analysis of Randomized Controlled Trials of Acupuncture versus Western Medicine in the Treatment of Insomnia

Zhang¹,

Dong²,

Hong³

et al. 2021

IJPS

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Acupuncture versus Western Medicine in the Treatment of Insomnia To evaluate the clinical effect and effectiveness of acupuncture therapy in the treatment of insomnia patients, so as to provide better evidence based services, benefit patients and promote it in clinical practice. Chinese databases were searched: China National Knowledge Infrastructure, Chinese Scientific Journals Full text Database, Wanfang data knowledge service platform; English databases were searched: PubMed, Cochrane Library, Embase. Stata 15.1 and RevMan 5.4.1 software were used for statistical analysis. A total of 22 randomized controlled trials (1572 cases) were included, including 780 in the experimental group and 792 in the control group. An analysis showed that the effective rate of acupuncture treatment for insomnia was better than that of Western medicine treatment and the combined effect odds ratio=1.23, 95 % confidence interval [1.17, 1.29]. Pure acupuncture group in Pittsburgh sleep quality experienced surpasses the Western medicine group evaluation combined effect of weighted mean difference=2.04, 95 % confidence interval [1.62, 2.46], p<0.01; In each score of Pittsburgh sleep quality index, sleep quality weighted mean difference=-0.27, 95 % confidence interval [-0.40, -0.13], p<0.01, time to fall asleep weighted mean difference=-0.21, 95 % confidence interval [-0.37, -0.06], p<0.01, sleep efficiency weighted mean difference=-0.30, 95 % confidence interval [-0.42, -0.19], p<0.01, sleep disorder weighted mean difference=-0.22, 95 % confidence interval [-0.41, -0.04], p<0.01, hypnotic drugs weighted mean difference=-0.79, 95 % confidence interval [-1.01, -0.57], p<0.01, daytime function weighted mean difference=-0.39, 95 % CI [-0.59, -0.19], p<0.01. All of the acupuncture groups were better than the Western medicine groups. In the sleep time weighted mean difference=-0.30, 95 % confidence interval [-0.05, 0.18], p=0.73, there was no statistical significance between the acupuncture group and the Western medicine group. Pure acupuncture has more advantages than Western medicine in the treatment of insomnia and it is suitable for clinical promotion.

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Combinatorial effects of conception and governor vessel electroacupuncture and human umbilical cord blood-derived mesenchymal stem cells on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion

Cited by 7 publications

References 21 publications

Assessment of Neuroprotective Properties ofMelissa officinalisin Combination With Human Umbilical Cord Blood Stem Cells After Spinal Cord Injury

Assessment of Neuroprotective Properties ofMelissa officinalisin Combination With Human Umbilical Cord Blood Stem Cells After Spinal Cord Injury

Growth differentiation factor 15 may protect the myocardium from no-reflow by inhibiting the inflammatory-like response that predominantly involves neutrophil infiltration

Meta-Analysis of Randomized Controlled Trials of Acupuncture versus Western Medicine in the Treatment of Insomnia

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