Combinatorial control of Arabidopsis proline dehydrogenase transcription by specific heterodimerisation of bZIP transcription factors

Weltmeier, Fridtjof; Ehlert, Andrea; Mayer, Caroline S.; Dietrich, Katrin; Wang, Xuan; Schütze, Katia; Alonso, Rosario; Harter, Klaus; Vicente‐Carbajosa, Jesús; Dröge‐Laser, Wolfgang

doi:10.1038/sj.emboj.7601206

Cited by 203 publications

(233 citation statements)

References 60 publications

(93 reference statements)

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“…5). A previous study has shown that group S1 proteins, including AtbZIP1, can form heterodimers with group C bZIP proteins to directly regulate the Arabidopsis Pro dehydrogenase gene (ProDH; At3g30775; Satoh et al, 2004;Weltmeier et al, 2006). Both AtbZIP1 and ProDH were found as RGA up-regulated and KIN10-induced genes in this study, indicating that AtbZIP1 is probably relevant to RGA regulation of floral organ development in response to stress.…”

Section: Expression Analysis Of Immediate Targets Of Rgamentioning

confidence: 59%

“…6, A-C). A previous study has shown that AtbZIP1 forms a heterodimer with group C bZIP proteins to activate the expression of ProDH (At3g30775), which is induced in response to hypoosmotic conditions occurring during rehydration after stress recovery (Satoh et al, 2004;Weltmeier et al, 2006). Interestingly, both AtbZIP1 and ProDH are RGA up-regulated and KIN10-induced genes.…”

Section: Rga Regulates Flower Development By Activating Stress-relatementioning

confidence: 99%

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Global Identification of DELLA Target Genes during Arabidopsis Flower Development

Hou

Shen

et al. 2008

Plant Physiology

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Gibberellin (GA) plays important roles in regulating many aspects of plant development. GA derepresses its signaling pathway by promoting the degradation of DELLA proteins, a family of nuclear growth repressors. Although the floral organ identity is established in flowers of the GA-deficient mutant ga1-3, the growth of all floral organs is severely retarded. In particular, abortive anther development in ga1-3 results in male sterility. Genetic analysis has revealed that various combinations of null mutants of DELLA proteins could gradually rescue floral organ defects in ga1-3 and that RGA is the most important DELLA protein involved in floral organ development. To elucidate the early molecular events controlled by RGA during flower development, we performed whole-genome microarray analysis to identify genes in response to the steroid-inducible activation of RGA in ga1-3 rgl2 rga 35S:RGA-GR. Although DELLA proteins were suggested as transcriptional repressors, similar numbers of genes were down-regulated or up-regulated by RGA during floral organ development. More than one-third of RGA down-regulated genes were specifically or predominantly expressed in stamens. A significant number of RGA-regulated genes are involved in phytohormone signaling or stress response. Further expression analysis through activation of RGA by steroid induction combined with cycloheximide identified eight genes as immediate targets of RGA. In situ hybridization and transgenic studies further showed that the expression pattern and function of several selected genes were consistent with the predictions from microarray analysis. These results suggest that DELLA regulation of floral organ development is modulated by multiple phytohormones and stress signaling pathways.

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Section: Expression Analysis Of Immediate Targets Of Rgamentioning

confidence: 59%

Section: Rga Regulates Flower Development By Activating Stress-relatementioning

confidence: 99%

Global Identification of DELLA Target Genes during Arabidopsis Flower Development

Hou

Shen

et al. 2008

Plant Physiology

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“…The formation of all combinations of homo-and heterodimers in the GeBP/ GPL family means they potentially have a high combinatorial flexibility to regulate target genes, which are so far unidentified. Indeed, in plants, heterodimeric protein interactions mediated by the Leu-zipper motif increase the repertoire of potential DNA-protein interactions (Weltmeier et al, 2006). This work allows the classification of GeBP/GPL as Leu-zipper proteins.…”

Section: Gebp/glp Proteins Are Noncanonical Leu-zipper Tfsmentioning

confidence: 94%

GeBP and GeBP-Like Proteins Are Noncanonical Leucine-Zipper Transcription Factors That Regulate Cytokinin Response in Arabidopsis

et al. 2007

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Understanding the role of transcription factors (TFs) is essential in reconstructing developmental regulatory networks. The plant-specific GeBP TF family of Arabidopsis thaliana (Arabidopsis) comprises 21 members, all of unknown function. A subset of four members, the founding member GeBP and GeBP-like proteins (GPL) 1, 2, and 3, shares a conserved C-terminal domain. Here we report that GeBP/GPL genes represent a newly defined class of leucine-zipper (Leu-zipper) TFs and that they play a redundant role in cytokinin hormone pathway regulation. Specifically, we demonstrate using yeast, in vitro, and split-yellow fluorescent protein in planta assays that GeBP/GPL proteins form homo-and heterodimers through a noncanonical Leu-zipper motif located in the C-terminal domain. A triple loss-of-function mutant of the three most closely related genes gebp gpl1 gpl2 shows a reduced sensitivity to exogenous cytokinins in a subset of cytokinin responses such as senescence and growth, whereas root inhibition is not affected. We find that transcript levels of type-A cytokinin response genes, which are involved in the negative feedback regulation of cytokinin signaling, are higher in the triple mutant. Using a GPL version that acts as a constitutive transcriptional activator, we show that the regulation of Arabidopsis response regulators (ARRs) is mediated by at least one additional, as yet unknown, repressor acting genetically downstream in the GeBP/GPL pathway. Our results indicate that GeBP/GPL genes encode a new class of unconventional Leu-zipper TF proteins and suggest that their role in the cytokinin pathway is to antagonize the negative feedback regulation on ARR genes to trigger the cytokinin response.

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“…We have shown in previous work and in this study that bZIP53 heterodimerization with bZIP10 or bZIP25 increases its transactivation properties Weltmeier et al, 2006). To determine if the heterodimerization was also affecting the binding capabilities of these proteins, we incubated increasing amounts of T7-bZIP53 in the wild-type oligo-coated wells in the presence or absence of fixed amounts of bZIP10.…”

Section: Activation Of the 2s2 Seed Storage Protein Gene By Bzip53 Anmentioning

confidence: 99%

“…We have previously shown that Arabidopsis S1 bZIP proteins support strong heterodimerization with members of the group C bZIPs using two-hybrid systems in yeast and protoplasts and bimolecular fluorescence complementation (BiFC) techniques Weltmeier et al, 2006). Since two group C members, bZIP10 and bZIP25, are known activators of SSP genes (Lara et al, 2003), we surveyed the expression patterns of S1 members during seed development to identify possible partners for C-S1 interactions in this process.…”

Section: Bzip53 Is Expressed In Seed Tissues During Maturationmentioning

confidence: 99%

A Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation ofArabidopsisSeed Maturation Gene Expression Based on Heterodimerization and Protein Complex Formation

et al. 2009

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Transcription of Arabidopsis thaliana seed maturation (MAT) genes is controlled by members of several transcription factor families, such as basic leucine zippers (bZIPs), B3s, MYBs, and DOFs. In this work, we identify Arabidopsis bZIP53 as a novel transcriptional regulator of MAT genes. bZIP53 expression in developing seeds precedes and overlaps that of its target genes. Gain-and loss-of-function approaches indicate a correlation between the amount of bZIP53 protein and MAT gene expression. Specific in vivo and in vitro binding of bZIP53 protein to a G-box element in the albumin 2S2 promoter is demonstrated. Importantly, heterodimerization with bZIP10 or bZIP25, previously described bZIP regulators of MAT gene expression, significantly enhances DNA binding activity and produces a synergistic increase in target gene activation. Fulllevel target gene activation is strongly correlated with the ratio of the correspondent bZIP heterodimerization partners. Whereas bZIP53 does not interact with ABI3, a crucial transcriptional regulator in Arabidopsis seeds, ternary complex formation between the bZIP heterodimers and ABI3 increases the expression of MAT genes in planta. We therefore propose that heterodimers containing bZIP53 participate in enhanceosome formation to produce a dramatic increase in MAT gene transcription.

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Combinatorial control of Arabidopsis proline dehydrogenase transcription by specific heterodimerisation of bZIP transcription factors

Cited by 203 publications

References 60 publications

Global Identification of DELLA Target Genes during Arabidopsis Flower Development

Global Identification of DELLA Target Genes during Arabidopsis Flower Development

GeBP and GeBP-Like Proteins Are Noncanonical Leucine-Zipper Transcription Factors That Regulate Cytokinin Response in Arabidopsis

A Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation ofArabidopsisSeed Maturation Gene Expression Based on Heterodimerization and Protein Complex Formation

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