Combinatorial coding of Drosophila muscle shape by Collier and Nautilus

Enriquez, Jonathan; Taffin, Mathilde de; Crozatier, Michèle; Víncent, Alaín; Dubois, L.

doi:10.1016/j.ydbio.2011.12.018

Cited by 26 publications

(53 citation statements)

References 60 publications

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“…Besides the support of haemocytes, collier is also required for the formation of the dorsal/acute 3 (DA3) embryonic muscle, which does not develop in its absence 46,47 . Together with nautilus, the only homologue of MRF bHLH factors in Drosophila, collier controls shape and morphology of the DA3 muscle 48 . Here we report that Ebf3 is required for the relaxation of the diaphragm and that it synergises with MyoD in inducing a critical cell-type specific gene.…”

Section: Discussionmentioning

confidence: 99%

Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1

et al. 2014

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Myogenic regulatory factors such as MyoD and Myf5 lie at the core of vertebrate muscle differentiation. However, E-boxes, the cognate binding sites for these transcription factors, are not restricted to the promoters/enhancers of muscle cell-specific genes. Thus, the specificity in myogenic transcription is poorly defined. Here we describe the transcription factor Ebf3 as a new determinant of muscle cell-specific transcription. In the absence of Ebf3 the lung does not unfold at birth, resulting in respiratory failure and perinatal death. This is due to a hypercontractile diaphragm with impaired Ca 2 þ efflux-related muscle functions. Expression of the Ca 2 þ pump Serca1 (Atp2a1) is downregulated in the absence of Ebf3, and its transgenic expression rescues this phenotype. Ebf3 binds directly to the promoter of Atp2a1 and synergises with MyoD in the induction of Atp2a1. In skeletal muscle, the homologous family member Ebf1 is strongly expressed and together with MyoD induces Atp2a1. Thus, Ebf3 is a new regulator of terminal muscle differentiation in the diaphragm, and Ebf factors cooperate with MyoD in the induction of muscle-specific genes.

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Section: Discussionmentioning

confidence: 99%

Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1

et al. 2014

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“…Stripe-GAL4 flies were provided by G. Morata (CBMSO, Universidad Autonoma de Madrid, Madrid, Spain). Collier-GFP flies were a gift from Dr Alan Vincent (Centre de Biologie du Développement, UMR 5547 and IFR 109, CNRS and Université Paul Sabatier) (Dubois et al, 2007;Enriquez et al, 2012). Homozygous embryos were recognized by the lack of either CyO,YFP or CyO,Wg-lacZ.…”

Section: Fly Strainsmentioning

confidence: 99%

A non-signaling role of Robo2 in tendons is essential for Slit processing and muscle patterning

Ordan

Volk

2015

Development

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Coordinated locomotion of an organism relies on the development of proper musculoskeletal connections. In Drosophila, the Slit-Robo signaling pathway guides muscles to tendons. Here, we show that the Slit receptor Roundabout 2 (Robo2) plays a non-cell-autonomous role in directing muscles to their corresponding tendons. Robo2 is expressed by tendons, and its non-signaling activity in these cells promotes Slit cleavage, producing a cleaved Slit N-terminal guidance signal that provides short-range signaling into muscles. Consistently, robo2 mutant embryos exhibited a muscle phenotype similar to that of slit, which could not be rescued by muscle-specific Robo2 expression but rather by ectodermally derived Robo2. Alternatively, this muscle phenotype could be induced by tendon-specific robo2 RNAi. We further show that membrane immobilization of Slit or its Nterminal cleaved form (Slit-N) on tendons bypasses the functional requirement for Robo2 in tendons, verifying that the major role of Robo2 is to promote the association of Slit with the tendon cell membrane. Slit-N tends to oligomerize whereas full-length uncleavable Slit does not. It is therefore proposed that Slit-N oligomers, produced at the tendon membrane by Robo2, signal to the approaching muscle by combined Robo1 and Robo3 activity. These findings establish a Robo2-mediated mechanism, independent of signaling, that is essential to limiting Slit distribution and which might be relevant to the regulation of Slit-mediated shortrange signaling in additional systems.

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“…As muscle identity is conferred by activation of specific combinations of iTFs in each PC, we looked at Tup expression at the PC stage. As general marker of PCs/FCs, we used rp298lacZ (Beckett and Baylies, 2007;Enriquez et al, 2012;Nose et al, 1998). Staining of late stage 11 rp298lacZ embryos for lacZ and either Tup or Col, showed that Tup expression is expressed in the four dorsal-most PCs ( Fig.…”

Section: Tup: a New Muscle Identity Transcription Factormentioning

confidence: 99%

Tup/Islet1 integrates time and position to specify muscle identity in Drosophila

et al. 2012

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SUMMARYThe LIM-homeodomain transcription factor Tailup/Islet1 (Tup) is a key component of cardiogenesis in Drosophila and vertebrates. We report here an additional major role for Drosophila Tup in specifying dorsal muscles. Tup is expressed in the four dorsal muscle progenitors (PCs) and tup-null embryos display a severely disorganized dorsal musculature, including a transformation of the dorsal DA2 into dorsolateral DA3 muscle. This transformation is reciprocal to the DA3 to DA2 transformation observed in collier (col) mutants. The DA2 PC, which gives rise to the DA2 muscle and to an adult muscle precursor, is selected from a cluster of myoblasts transiently expressing both Tinman (Tin) and Col. The activation of tup by Tin in the DA2 PC is required to repress col transcription and establish DA2 identity. The transient, partial overlap between Tin and Col expression provides a window of opportunity to distinguish between DA2 and DA3 muscle identities. The function of Tup in the DA2 PC illustrates how single cell precision can be reached in cell specification when temporal dynamics are combined with positional information. The contributions of Tin, Tup and Col to patterning Drosophila dorsal muscles bring novel parallels with chordate pharyngeal muscle development.

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Combinatorial coding of Drosophila muscle shape by Collier and Nautilus

Cited by 26 publications

References 60 publications

Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1

Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1

A non-signaling role of Robo2 in tendons is essential for Slit processing and muscle patterning

Tup/Islet1 integrates time and position to specify muscle identity in Drosophila

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