Combinations of putative virulence markers in typical and variant enteroaggregative <i>Escherichia coli</i> strains from children with and without diarrhoea

Elias, Waldir P.; Uber, Ana Paula; Tomita, Sandra Kimie; Trabulsi, Luíz Rachid; Gomes, Tânia A. T.

doi:10.1017/s0950268802007136

Cited by 58 publications

(79 citation statements)

References 24 publications

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“…Elias and others 44 reported aggR in 81% and astA in 48% of EAEC from diarrheic Brazilian children. Several workers reported relatively similar results.…”

Section: Discussionmentioning

confidence: 89%

“…A study from Brazil examined EAEC from diarrheic and control children for 11 virulence markers and found multivirulence in 74% of EAEC strains. 44 Differences in the reported rates of virulence genes among EAEC from different regions may be caused by differences in geography, source of fecal samples examined (e.g., from diarrheic children or adults), number of EAEC strains examined, and methods used (phenotypic or genotypic).…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of Diarrheagenic Escherichia coli from Libya

Ali¹,

Kamel²,

Klena³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Diarrheagenic Escherichia coli (DEC) are important enteric pathogens that cause a wide variety of gastrointestinal diseases, particularly in children. Escherichia coli isolates cultured from 243 diarrheal stool samples obtained from Libyan children and 50 water samples were screened by polymerase chain reaction (PCR) for genes characteristic of enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enterohemorrhagic E. coli (EHEC), and enteroinvasive E. coli (EIEC). The DEC were detected in 21 (8.6%) children with diarrhea; 10 (4.1%) cases were identified as EAEC, 3 (1.2%) as EPEC, and 8 (3.3%) were ETEC; EHEC, and EIEC were not detected. All DEC were grouped phylogenetically by PCR with the majority ( 70%) identified as phylogenetic groups A and B1. The EAEC isolates were also tested for eight genes associated with virulence using PCR. Multi-virulence ( 3 virulence factors) was found in 50% of EAEC isolates. Isolated EAEC possessed different virulence traits and belonged to different phylogenetic groups indicating their heterogeneity.

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“…Elias and others 44 reported aggR in 81% and astA in 48% of EAEC from diarrheic Brazilian children. Several workers reported relatively similar results.…”

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Diarrheagenic Escherichia coli from Libya

Ali¹,

Kamel²,

Klena³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…The pathogenesis of EAEC is complex, and EAEC strains are very heterogeneous (Elias et al, 2002). Additionally, human and animal studies indicate that EAEC is able to bind to jejunal, ileal and colonic epithelium.…”

Section: Discussionmentioning

confidence: 99%

Pet secretion, internalization and induction of cell death during infection of epithelial cells by enteroaggregative Escherichia coli

Betancourt-Sanchez

Navarro-Garcı́a

2009

Microbiology

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In an in vitro model using HEp-2 cells treated with purified plasmid-encoded toxin (Pet), we have identified morphological changes characterized by cell rounding and detachment after toxin internalization; these changes progress to cell death. However, these effects have not yet been shown to occur during the infection of epithelial cells by enteroaggregative Escherichia coli (EAEC). Here, we show that the secretion of Pet by EAEC is regulated at the transcriptional level, since secretion was inhibited in eukaryotic cell culture medium, although Pet was efficiently secreted in the same medium supplemented with tryptone. Inefficient secretion of Pet by EAEC in DMEM prevented cell detachment, whereas efficient Pet secretion in DMEM/tryptone increased cell detachment in a HEp-2 cell adherence assay. Interestingly, Pet toxin was efficiently delivered to epithelial cells, since it was internalized into epithelial cells infected with EAEC at similar concentrations to those obtained by using 37 mg ml "1 purified Pet protein. Additionally, Pet was not internalized when the epithelial cells were infected with a pet clone, HB101(pCEFN1), unlike the wild-type strain, which has a high adherence capability. There is a correlation between Pet secretion by EAEC, the internalization of Pet into epithelial cells, cell detachment and cell death in EAEC-infected cells. The ratio between live and dead cells decreased in cells treated with wildtype EAEC in comparison with cells treated with an isogenic mutant in the pet gene, whereas the effects were restored by complementing the mutant with the pet gene. All these data indicate that Pet is an important virulence factor in the pathogenesis of EAEC infection.

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“…UTI is one of the most common human infections influenced by gender and age [2,21]. REP-PCR reveals DNA patterns and in fact allows differentiation between bacterial species [22][23] making it a useful technique for typing various bacteria [23]. Non-coding dispersed repetitive sequences have been observed preferably in gramnegative bacteria [24].…”

Section: Profile Of Pcr Products Of Some Samples Column L Is the Sizmentioning

confidence: 99%

Molecular Typing of Uropathogenic Escherichia coli Strains Isolated from Patients by REP-PCR

Amiri¹,

Ranjbar²,

Sohrabi³

et al. 2017

Sovrem Tehnol Med

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The aim of the investigation was to determine the correlation between genetic patterns and strains of Escherichia coli (E. coli) and to evaluate the genetic diversity of E. coli strains isolated from patients admitted to Baqiyatallah Hospital, using the method of repetitive extragenic palindromic sequence polymerase chain reaction (REP-PCR).Materials and Methods. A total of 100 E. coli strains collected from the samples of patients with urinary tract infections were studied. E. coli strains were identified and isolated by the standard bacteriological methods and their DNAs were extracted. The REP-PCR method with REP1R primer was used to type isolated strains.Results. Ninety-eight out of 100 E. coli strains were typable and 2 strains were non-typable. Dendrogram showed that the isolated strains were differentiated by REP-PCR into 7 REP clusters (R1-R7) with 70% similarity. The highest number of strains was found in cluster R4 (22 strains) and this number was the lowest in cluster R3 (7 strains).Conclusion. In Iran, the REP-PCR method has not been used for studying uropathogenic E. coli strains. This technique has shown high differential accuracy in our investigation. E. coli strains have been found to comprise various distinct clones which show the genetic diversity of bacteria in the patients studied.

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Combinations of putative virulence markers in typical and variant enteroaggregative Escherichia coli strains from children with and without diarrhoea

Cited by 58 publications

References 24 publications

Molecular Characterization of Diarrheagenic Escherichia coli from Libya

Molecular Characterization of Diarrheagenic Escherichia coli from Libya

Pet secretion, internalization and induction of cell death during infection of epithelial cells by enteroaggregative Escherichia coli

Molecular Typing of Uropathogenic Escherichia coli Strains Isolated from Patients by REP-PCR

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