Combinations of DEAD box proteins distinguish distinct types of RNA: Protein complexes in neurons

Miller, Linda C.; Blandford, Vanessa; McAdam, Robyn; Sánchez-Carbente, María R.; Badeaux, Frédérique; DesGroseillers, Luc; Sossin, Wayne S.

doi:10.1016/j.mcn.2009.01.007

Cited by 35 publications

(27 citation statements)

References 27 publications

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“…All of these processing body components formed puncta located in the cell soma and dendrites (Fig. 1A-G and data not shown), as described previously by us and others (Cougot et al, 2008;Zeitelhofer et al, 2008;Miller et al, 2009;Thomas et al, 2011). For all these molecules, the number of foci along dendrites was comparable.…”

Section: Xrn1 Forms Synaptic Bodies That Are Different From Processinsupporting

confidence: 86%

“…Different types of processing bodies containing subsets of components were reported in dendrites and synapse surroundings (Cougot et al, 2008;Zeitelhofer et al, 2008;di Penta et al, 2009;Miller et al, 2009;Pradhan et al, 2012). Here, we show that XRN1 forms clusters associated with the post-synapse that we termed synaptic XRN1 bodies (SXbodies), as they do not contain the canonical processing body components DCP1A, Ge-1 (also known as Hedls, RCD8 or EDC4) or Rck (also known as p54 or DDX6) and are also different from the previously described Smaug1 (also known as Samd4a) foci (S-foci) and FMRP or survival of motor neuron (SMN1) granules.…”

Section: Introductionmentioning

confidence: 99%

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Synaptic control of mRNA translation by reversible assembly of XRN1 bodies

Luchelli¹,

Thomas²,

Boccaccio³

2015

Journal of Cell Science

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Repression of mRNA translation is linked to the formation of specific cytosolic foci such as stress granules and processing bodies, which store or degrade mRNAs. In neurons, synaptic activity regulates translation at the post-synapse and this is important for plasticity. Nmethyl-D-aspartate (NMDA) receptor stimulation downregulates translation, and we speculate that this is linked to the formation of unknown mRNA-silencing foci. Here, we show that the 59-39 exoribonuclease XRN1 forms discrete clusters associated with the post-synapse that are different from processing bodies or stress granules, and we named them synaptic XRN1 bodies (SX-bodies). Using primary neurons, we found that the SX-bodies respond to synapse stimulation and that their formation correlates inversely with the local translation rate. SX-bodies increase in size and number upon NMDA stimulation, and metabotropic glutamate receptor activation provokes SX-body dissolution, along with increased translation. The response is specific and the previously described Smaug1 foci and FMRP granules show a different response. Finally, XRN1 knockdown impairs the translational repression triggered by NMDA. Collectively, these observations support a role for the SX-bodies in the reversible masking and silencing of mRNAs at the synapse.

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Section: Xrn1 Forms Synaptic Bodies That Are Different From Processinsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Synaptic control of mRNA translation by reversible assembly of XRN1 bodies

Luchelli¹,

Thomas²,

Boccaccio³

2015

Journal of Cell Science

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“…The majority of the RBPs identified here were found to interact with only one or two of the four axonal mRNA localization motifs tested. MS analyses of proteins interacting with the RBPs Staufen and Barentsz in embryonic rat brain indicate at least two distinct dendritic RNP populations (60), and multiple dendritic RNPs have been defined by DEAD box protein content (68). Thus, the RBPs identified in the RAMS analyses here most likely constitute unique protein combinations for cohorts of axonally localizing mRNAs.…”

Section: Sj Lee Et Al Rna-protein Interactions For Axonal Mrnasmentioning

confidence: 70%

hnRNPs Interacting with mRNA Localization Motifs Define AxoNAl RNA Regulons

Lee

Oses-Prieto

Kawaguchi

et al. 2018

Molecular & Cellular Proteomics

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“…Remarkably, four out of the five proteins colocalized, albeit at different levels, with a YFP-labeled dFMR1 granules in neurites of cultured Drosophila motor neurons ( Figure 2; Barbee et al 2006). No specific antibody was available to test the presence of Dco/Dbt in neuronal granules but studies from Saccharomyces cerevisiae have shown that a homologous protein is present on P bodies (D. Muhlrad and R. Parker, unpublished observations), which contain many of the components found in neuronal granules (Barbee et al 2006;Cougot et al 2008;Miller et al 2009). Thus, we conclude that at least PABP, Orb2, Rm62, and SmD3 are components of FMRP-containing RNA granules found in neuronal processes.…”

Section: Resultsmentioning

confidence: 99%

Genetic Modifiers ofdFMR1Encode RNA Granule Components in Drosophila

Cziko¹,

McCann

Howlett³

et al. 2009

Genetics

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Mechanisms of neuronal mRNA localization and translation are of considerable biological interest. Spatially regulated mRNA translation contributes to cell-fate decisions and axon guidance during development, as well as to long-term synaptic plasticity in adulthood. The Fragile-X Mental Retardation protein (FMRP/dFMR1) is one of the best-studied neuronal translational control molecules and here we describe the identification and early characterization of proteins likely to function in the dFMR1 pathway. Induction of the dFMR1 in sevenless-expressing cells of the Drosophila eye causes a disorganized (rough) eye through a mechanism that requires residues necessary for dFMR1/FMRP's translational repressor function. Several mutations in dco, orb2, pAbp, rm62, and smD3 genes dominantly suppress the sev-dfmr1 rough-eye phenotype, suggesting that they are required for dFMR1-mediated processes. The encoded proteins localize to dFMR1-containing neuronal mRNPs in neurites of cultured neurons, and/or have an effect on dendritic branching predicted for bona fide neuronal translational repressors. Genetic mosaic analyses indicate that dco, orb2, rm62, smD3, and dfmr1 are dispensable for translational repression of hid, a microRNA target gene, known to be repressed in wing discs by the bantam miRNA. Thus, the encoded proteins may function as miRNA-and/or mRNA-specific translational regulators in vivo.

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Combinations of DEAD box proteins distinguish distinct types of RNA: Protein complexes in neurons

Cited by 35 publications

References 27 publications

Synaptic control of mRNA translation by reversible assembly of XRN1 bodies

Synaptic control of mRNA translation by reversible assembly of XRN1 bodies

hnRNPs Interacting with mRNA Localization Motifs Define AxoNAl RNA Regulons

Genetic Modifiers ofdFMR1Encode RNA Granule Components in Drosophila

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