Combinational transformation of three wheat genes encoding fructan biosynthesis enzymes confers increased fructan content and tolerance to abiotic stresses in tobacco

XiaoMin, Bie; Wang, Ke; She, Maoyun; Du, Liangcheng; Zhang, Shuangxi; Li, Jiarui; Gao, Xiang; Lin, Zhishan; Ye, Xingguo

doi:10.1007/s00299-012-1332-y

Cited by 37 publications

(26 citation statements)

References 34 publications

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“…Under abiotic stress, expression of many proteins related to oxidative stress, photosynthesis, carbohydrate metabolism and regulation are altered [22–25]. In order to examine how expression of PEPC was affected by abiotic stress, the T34 transgenic line was subjected to drought and high temperature stress.…”

Section: Resultsmentioning

confidence: 99%

Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene

et al. 2014

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Phosphoenolpyruvate carboxylase (PEPC) is known to play a key role in the initial fixation of CO2 in C4 photosynthesis. The PEPC gene from sugarcane (a C4 plant) was introduced into indica rice (Hang2), a process mediated by Agrobacterium tumefaciens. Integration patterns and copy numbers of the gene was confirmed by DNA blot analysis. RT-PCR and western blotting results showed that the PEPC gene was expressed at both the mRNA and protein levels in the transgenic lines. Real-time PCR results indicated that expression of the sugarcane PEPC gene occurred mostly in green tissues and changed under high temperature and drought stress. All transgenic lines showed higher PEPC enzyme activities compared to the untransformed controls, with the highest activity (11.1 times higher than the controls) being observed in the transgenic line, T34. The transgenic lines also exhibited higher photosynthetic rates. The highest photosynthetic rate was observed in the transgenic line, T54 (22.3 μmol m−2 s−1; 24.6 % higher than that in non-transgenic plants) under high-temperature conditions. Furthermore, the filled grain and total grain numbers for transgenic lines were higher than those for non-transgenic plants, but the grain filling (%) and 1,000-grain weights of all transgenic lines remained unchanged. We concluded that over-expression of the PEPC gene from sugarcane in indica rice (Hang2) resulted in higher PEPC enzyme activities and higher photosynthesis rates under high-temperature conditions.Electronic supplementary materialThe online version of this article (doi:10.1007/s11033-014-3070-4) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene

et al. 2014

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“…Different combinations of 1-SST, 6-SFT and 1-FFT genes for fructan production in transgenic tobacco plants had a significant influence on tolerance to abiotic stresses. The combination of 1-SST and 6-SFT produced the highest fructan or soluble sugar content and the strongest salt tolerance (Bie et al 2012). It can be assumed that there is a good potential for manipulation of these major genes in wheat so that they might raise the power of fructan production and remobilization during the grain filling period under salinity.…”

Section: Production and Remobilization Of Fructan And Their Possible mentioning

confidence: 99%

Wheat stem reserves and salinity tolerance: molecular dissection of fructan biosynthesis and remobilization to grains

Sharbatkhari

Shobbar

Galeshi

et al. 2016

Planta

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Fructan accumulation and remobilization to grains under salinity can decrease dependency of the wheat tolerant cultivar on current photosynthesis and protect it from severe yield loss under salt stress. Tolerance of plants to abiotic stresses can be enhanced by accumulation of soluble sugars, such as fructan. The current research sheds light on the role of stem fructan remobilization on yield of bread wheat under salt stress conditions. Fructan accumulation and remobilization as well as relative expression of the major genes of fructan metabolism were investigated in the penultimate internodes of 'Bam' as the salt-tolerant and 'Ghods' as the salt-sensitive wheat cultivars under salt-stressed and controlled conditions and their correlations were analyzed. More fructan production and higher efficiency of fructan remobilization was detected in Bam cultivar under salinity. Up-regulation of sucrose: sucrose 1-fructosyltransferase (1-SST) and sucrose: fructan 6-fructosyltransferase (6-SFT) (fructan biosynthesis genes) at anthesis and up-regulation of fructan exohydrolase (1-FEH) and vacuolar invertase (IVR) genes (contributed to fructan metabolism) during grain filling stage and higher expression of sucrose transporter gene (SUT1) in Bam was in accordance with its induced fructan accumulation and remobilization under salt stress. A significant correlation was observed between weight density, WSCs and gene expression changes under salt stress. Based on the these results, increased fructan production and induced stem reserves remobilization under salinity can decrease dependency of the wheat tolerant cultivar on current photosynthesis and protect it from severe yield loss under salt stress conditions.

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“…In fructan species, fructan accumulation can be induced under drought (De Roover et al, 2000) and cold (Livingston and Henson, 1998; Yoshida et al, 2007). More direct evidence comes from the observation that fructan-accumulating transgenic plants show enhanced stress tolerance (Pilon-Smits et al, 1995, 1999; Konstantinova et al, 2002; Li et al, 2007; Kawakami et al, 2008; Bie et al, 2012). Transgenic perennial ryegrass expressing wheat 1-SST or 6-SFT genes accumulate more fructans and acquired higher tolerance for freezing at the cellular level (Hisano et al, 2004).…”

Section: Classic Functions Of Fructans and Rfosmentioning

confidence: 99%

Multifunctional fructans and raffinose family oligosaccharides

Ende¹

2013

Front. Plant Sci.

192

116

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Fructans and raffinose family oligosaccharides (RFOs) are the two most important classes of water-soluble carbohydrates in plants. Recent progress is summarized on their metabolism (and regulation) and on their functions in plants and in food (prebiotics, antioxidants). Interest has shifted from the classic inulin-type fructans to more complex fructans. Similarly, alternative RFOs were discovered next to the classic RFOs. Considerable progress has been made in the understanding of structure–function relationships among different kinds of plant fructan metabolizing enzymes. This helps to understand their evolution from (invertase) ancestors, and the evolution and role of so-called “defective invertases.” Both fructans and RFOs can act as reserve carbohydrates, membrane stabilizers and stress tolerance mediators. Fructan metabolism can also play a role in osmoregulation (e.g., flower opening) and source–sink relationships. Here, two novel emerging roles are highlighted. First, fructans and RFOs may contribute to overall cellular reactive oxygen species (ROS) homeostasis by specific ROS scavenging processes in the vicinity of organellar membranes (e.g., vacuole, chloroplasts). Second, it is hypothesized that small fructans and RFOs act as phloem-mobile signaling compounds under stress. It is speculated that such underlying antioxidant and oligosaccharide signaling mechanisms contribute to disease prevention in plants as well as in animals and in humans.

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Combinational transformation of three wheat genes encoding fructan biosynthesis enzymes confers increased fructan content and tolerance to abiotic stresses in tobacco

Cited by 37 publications

References 34 publications

Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene

Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene

Wheat stem reserves and salinity tolerance: molecular dissection of fructan biosynthesis and remobilization to grains

Multifunctional fructans and raffinose family oligosaccharides

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