Combination of vitamin E and L-carnitine is superior in protection against isoproterenol-induced cardiac injury: histopathological evidence

Huwait, Etimad

doi:10.5603/fm.a2018.0070

Cited by 7 publications

(12 citation statements)

References 24 publications

(27 reference statements)

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“…In another rat study of ALCAR, the agent reduced cardiac interfiber bleeding and cardiac fiber atrophy. 19 Altun et al 20 observed that combined treatment with ALCAR and CDDP decreased autotoxic effects by approximately 22%. Handzlik et al 21 found that ALCAR decreased these effects by 59% under acute hypoxic conditions.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant effect of acetyl-l-carnitine against cisplatin-induced cardiotoxicity

et al. 2020

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Objective Cisplatin (CDDP) toxicity is a dose-limiting clinical problem in clinical practice, mainly because of nephrotoxicity or ototoxicity. However, the mechanism of CDDP-induced cardiotoxicity is poorly understood. Acetyl-l-carnitine (ALCAR) is an antioxidant agent with protective effects against the side effects of various chemotherapeutics. CDDP-induced cardiotoxicity and the protective role of ALCAR were evaluated in this study. Methods Morphological changes were evaluated in hematoxylin and eosin-stained sections, and immunohistochemistry for caspase-3, superoxide dismutase-2 (SOD-2), inducible nitrite oxide synthase (iNOS), cyclooxygenase-2, and Bcl-2 was performed using the hearts of athymic nude mice carrying xenograft neuroblastoma tumors. Mice were randomized (six/group) to the control, CDDP (16 mg/kg), and ALCAR (200 mg/kg)+CDDP (16 mg/kg) groups. Results were analyzed using nonparametric tests. Results No difference was observed in the rates of cardiac necrosis, dilated/congested blood vessels, hemorrhage, polymorphonuclear leukocyte infiltration, edema, and pyknotic nuclei among the groups. SOD-2 expression was increased in the CDDP group but not in the ALCAR+CDDP group. iNOS, Bcl-2, and caspase-3 levels were not significantly different among the groups. Conclusions ALCAR might be a candidate protective agent for CDDP-induced cardiotoxicity. SOD-2, as a member of the oxidant system, should be evaluated in further studies as a biomarker of cardiotoxicity.

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Section: Discussionmentioning

confidence: 99%

Antioxidant effect of acetyl-l-carnitine against cisplatin-induced cardiotoxicity

et al. 2020

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“…Rather than attempting to modify one nutrient at a time, the diet tested in this study contained a cocktail of multiple supplemental nutrients (Table 1). Previous studies have shown that combinations of nutrients can perform better than single nutrient supplements [38, 39]. A blinded, randomized placebo-controlled interventional study was conducted, using echocardiogram evaluation as the primary endpoint, to evaluate the effect of the diet on progression of early stage MMVD in dogs.…”

Section: Introductionmentioning

confidence: 99%

Dietary intervention reduces left atrial enlargement in dogs with early preclinical myxomatous mitral valve disease: a blinded randomized controlled study in 36 dogs

Li¹,

Heaney²,

Langenfeld-McCoy³

et al. 2019

BMC Vet Res

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BackgroundMyxomatous mitral valve disease (MMVD), the most common naturally-occurring heart disease in dogs, is associated with alterations in energy metabolism, oxidative stress and inflammation. Energy deprivation plays a causal role in the development of heart failure. This study was designed to determine if a cardiac protection blend (CPB) of nutrients containing medium-chain triglycerides as an alternative energy source, fish oil to reduce inflammation, antioxidants, and other key nutrients important to cardiac health and function could slow or prevent MMVD progression. Nineteen dogs with early stage MMVD and 17 breed-, age-, and sex-matched healthy dogs were enrolled for a 6-month blinded, placebo-controlled study. Dogs in each cardiac health group were randomly assigned to either control diet (CON) or CPB-supplemented diet. Echocardiography was performed at baseline, 3 months and 6 months.ResultsNo changes were found in healthy dogs. While MMVD-CON dogs had an average 10% increase over baseline in left atrial diameter (LAD) and left atrial to aortic root ratio (LA/Ao) at 6 months, MMVD-CPB dogs showed 3% decreases, resulting significant diet by time interactions (P = 0.037, P = 0.005, respectively). More MMVD-CON dogs progressed from stage B1 to B2 during the study. A positive correlation was found between 6-month changes in LAD and blood pressures in MMVD-CPB dogs (systolic: P = 0.050, diastolic: P = 0.035) but not MMVD-CON dogs.ConclusionsOur results demonstrated efficacy of CPB-based dietary intervention in reducing LA size and mitral regurgitation, and in slowing or preventing the progression of early MMVD in dogs.

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“…ISO group showed significantly elevated cTnI, LDH and CKMB compared to the control which matches numerous former studies [1,[22][23][24][25][26] . This marked increase in serum level of cardiac enzymes denotes necrotic damage and leakiness of myocardial cell membrane [23,27] and could be explained by the enhanced production of free radicals with subsequent lysosomal degradation and alteration of myocardial structure as well as function [28] .…”

Section: Discussionmentioning

confidence: 99%

“…The edema was manifested in this work by the wide separations between myofibers and may explain the significant increase in the ventricular diameter and HWI detected in ISO group. Furthermore, it was proved that ISO administration in rats resulted in dilated congested or ruptured capillaries with hemorrhagic foci between the fibers followed by perivascular inflammatory cells [28] .…”

Section: One Of the Major Regulators Of Autophagy Is Ampk;mentioning

confidence: 99%

Cardioprotective Potential of Zinc and Vitamin E Against Isoprenaline-Induced Myocardial Infarction in Albino Rats by Targeting Autophagy: A Histological and Biochemical Study

Ismail

ShamsEldeen

Rashed

et al. 2020

Egyptian Journal of Histology

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Background: Myocardial infarction (MI) is a leading cause of morbidity and mortality. It is associated with oxidative stress, apoptosis and inflammation. Zinc (Zn) and vitamin E (VE) are known to exert antioxidant and anti-inflammatory effects. Aim of the Work: Evaluate the cardioprotective potential of Zn, VE and their combination against isoprenaline (ISO)induced myocardial infarction in adult male albino rats. Materials and Methods: Forty rats divided into five groups; I (control), II (ISO group): rats were injected subcutaneously (SC) with ISO (100 mg/kg) on the 20th and 21st days at interval of 24 h. Groups III (Zn group), IV (VE group) and V (ZE group): rats received respectively daily Zn (30 mg/kg), VE (100 mg/kg) or combination of both orally for 21 days and injected with ISO as group II. On the 22nd day, electrocardiography, biochemical and histological studies were done. Myocardial sections were subjected to H&E, caspase-3 and beclin 1 immunohistochemical stains. This was followed by morphometric and statistical analysis. Results: Group II exhibited significant electrocardiographic and biochemical changes compared to the control; deterioration of cardiac function with elevated cardiac enzymes, MDA, TNF-α and mTOR, in addition to reduced SOD, IL-10 and AMPK. Myocardial sections showed disturbed architecture with marked inflammatory infiltration and significantly increased caspase-3 and decreased beclin 1 immunoexpression. Groups III and IV revealed decreased cardiac enzymes, MDA, TNF-α and mTOR, in addition to elevated SOD, IL-10 and AMPK. Myocardial sections showed nearly normal histology with significantly decreased caspase-3 and increased beclin 1 immunoexpression. Group V presented the most protection with results significant to both groups III and IV. Conclusion: Combined Zn and VE pretreatment proved to have protective effect against ISO-induced MI more than using either of them alone regarding the electrocardiography, biochemical and histological parameters and this was through targeting autophagy and modulating its AMPK-mTOR pathway.

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Combination of vitamin E and L-carnitine is superior in protection against isoproterenol-induced cardiac injury: histopathological evidence

Abstract: Background: L-carnitine and Vitamin E have antioxidant properties. This study aimed to assess

Cited by 7 publications

References 24 publications

Antioxidant effect of acetyl-l-carnitine against cisplatin-induced cardiotoxicity

Antioxidant effect of acetyl-l-carnitine against cisplatin-induced cardiotoxicity

Dietary intervention reduces left atrial enlargement in dogs with early preclinical myxomatous mitral valve disease: a blinded randomized controlled study in 36 dogs

Cardioprotective Potential of Zinc and Vitamin E Against Isoprenaline-Induced Myocardial Infarction in Albino Rats by Targeting Autophagy: A Histological and Biochemical Study

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