Combination of treadmill exercise with bone marrow stromal cells transplantation activates protein synthesis-related molecules in soleus muscle of the spinal cord injured rats

Kim, You-Mi; Ji, Eun‐Sang; Ko, Il‐Gyu; Jin, Jianguo; Cho, Yeong-Hyun; Seo, Tae-Beom

doi:10.12965/jer.1938284.142

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…Previous clinical studies have reported that rehabilitative training increases physical activity levels and enhances the quality of life in people with SCI [ 7 – 11 ]. The mechanisms contributing to recovery have been partially demonstrated by facilitating synaptic plasticity [ 12 , 13 ], promoting the expression of neurotrophins such as brain-derived neurotrophic factor (BDNF) and insulin-like growth factor 1 (IGF-1) [ 14 – 17 ], and then activating the downstream signaling pathway such as mTOR [ 14 , 18 , 19 ]. However, previous studies suggest that rehabilitative training alone have cannot promote regrowth of the CST axons [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Osteopontin enhances the effect of treadmill training and promotes functional recovery after spinal cord injury

Wang,

Su,

Zhong

et al. 2023

Mol Biomed

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In this study, we examined the combined impact of osteopontin (OPN) and treadmill training on mice with spinal cord injury (SCI). OPN was overexpressed by injecting AAV9-SPP1-GFP into the sensorimotor cortex, followed by a left incomplete C5 crush injury two weeks later. Mice (Ex or Ex + OPN group) were trained at 50% maximum running speed for 8 weeks. To analyze the effects, we used biotinylated dextran amine (BDA) for tracing the corticospinal tract (CST) and performed Western blotting and immunohistochemical methods to assess the activation of the mammalian target of rapamycin (mTOR). We also examined axonal regeneration and conducted behavioral tests to measure functional recovery. The results demonstrated that treadmill training promoted the expression of neurotrophic factors such as brain-derived neurotrophic factor (BNDF) and insulin-like growth factor I (IGF-1) and activated mTOR signaling. OPN amplified the effect of treadmill training on activating mTOR signaling indicated by upregulated phosphorylation of ribosomal protein S6 kinase (S6). The combination of OPN and exercise further promoted functional recovery and facilitated limited CST axonal regeneration which did not occur with treadmill training and OPN treatment alone. These findings indicate that OPN enhances the effects of treadmill training in the treatment of SCI and offer new therapeutic insights for spinal cord injury.

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Section: Introductionmentioning

confidence: 99%

Osteopontin enhances the effect of treadmill training and promotes functional recovery after spinal cord injury

Wang,

Su,

Zhong

et al. 2023

Mol Biomed

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“…SCI model was induced according to the previously described method [12]. The rats were anesthetized by inhalation of isoflurane (2% isoflurane in 30% O2 and 70% N2, JW Pharmaceutical, Seoul, Korea) during surgery.…”

Section: Inducing Sci and Treatmentmentioning

confidence: 99%

Evaluation of PTEN Inhibitor Following Spinal Cord Injury on Recovery of Voiding Efficiency and Motor Function Observed by Regeneration in Spinal Cord

Cho

Kim

2020

Int Neurourol J

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Purpose: Neurogenic bladder (NB) associated with spinal cord injury (SCI) is a serious health problem. However, no effective treatment has been developed for SCI patients with NB. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) inhibitors have been proposed as a promising option for inducing neural regeneration. Therefore, we investigated the effects of a tissue gene nerve (TGN), PTEN inhibitor, on voiding function, motor function, and the expression of growth factors after SCI.Methods: In this experiment, female rats were randomly divided into 3 groups (n=10 in each group): the sham-operation group, the SCI-induced group, and the SCI-induced and TGN-treated group. Cystometry; the Basso, Beattie, and Bresnahan (BBB) scale test; the ladder walking test; hematoxylin and eosin staining; and Western blotting for brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF), and nerve growth factor (NGF) were performed to evaluate functional and molecular changes.Results: After SCI, the rats exhibited decreased walking ability according to the BBB scale test and impaired coordinative function according to the ladder walking test. The PTEN inhibitor promoted enhanced walking ability and coordinative function. Cystometry showed voiding impairment after SCI and improved voiding function was observed after PTEN treatment. Overexpression of VEGF, BDNF, and NGF were observed after SCI. Administration of PTEN inhibitors significantly attenuated the overexpression of growth factors due to SCI.Conclusion: PTEN inhibitor treatment diminished the overexpression of growth factors and promoted the repair of damaged tissue. PTEN inhibitor-treated rats also showed improved motor function and improved voiding function. Therefore, we suggest TGN as a new therapeutic agent that can be applied after SCI.

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“…Exercise promotes skeletal muscle health in many ways. It affects multiple physiological processes involved in molecular, cellular, and biochemical pathways regulating skeletal muscle gene expression and the metabolic process, thereby promoting the health of skeletal muscle (11,20,27). Sarcomeres are represented as the elemental contractile units of striated muscles and are composed of thick filaments, thin filaments, and titin filaments.…”

Section: Introductionmentioning

confidence: 99%

Scriptaid/exercise-induced lysine acetylation is another type of posttranslational modification occurring in titin

Huang

Liu

Niu

et al. 2020

Journal of Applied Physiology

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Titin serves important functions in skeletal muscle during exercise, and posttranslational modifications of titin participate in the regulation of titin-based sarcomeric functions. Scriptaid has exercise-like effects through the inhibition of HDAC and regulatory acetylation of proteins. However, it remains mostly unclear if exercise could result in titin’s acetylation and whether Scriptaid could regulate acetylation of titin. We treated C57BL/6 mice with 6-wk treadmill exercise and 6-wk Scriptaid administration to explore Scriptaid’s effects on mice exercise capacity and whether Scriptaid administration/exercise could induce titin’s acetylation modification. An exercise endurance test was conducted to explore their effects on mice exercise capacity, and proteomic studies were conducted with gastrocnemius muscle tissue of mice from different groups to explore titin’s acetylation modification. We found that Scriptaid and exercise did not change titin’s protein expression, but they did induce acetylation modification changes of titin. In total, 333 acetylated lysine sites were identified. Exercise changed the acetylation levels of 33 lysine sites of titin, whereas Scriptaid changed acetylation levels of 31 titin lysine sites. Exercise treatment and Scriptaid administration shared 11 lysine sites. In conclusion, Scriptaid increased exercise endurance of mice by increasing the time mice spent running to fatigue. Acetylation is a common type of posttranslational modification of titin, and exercise/Scriptaid changed the acetylation levels of titin and titin-interacting proteins. Most importantly, titin may be a mediator through which Scriptaid and exercise modulate the properties and functions of exercise-induced skeletal muscle at the molecular level. NEW & NOTEWORTHY Scriptaid administration increased mouse exercise endurance. Acetylation is another type of posttranslational modification of titin. Scriptaid/exercise changed acetylation levels of titin and titin-interacting proteins. Titin may mediate exercise-induced skeletal muscle properties and functions.

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Combination of treadmill exercise with bone marrow stromal cells transplantation activates protein synthesis-related molecules in soleus muscle of the spinal cord injured rats

Cited by 4 publications

References 22 publications

Osteopontin enhances the effect of treadmill training and promotes functional recovery after spinal cord injury

Osteopontin enhances the effect of treadmill training and promotes functional recovery after spinal cord injury

Evaluation of PTEN Inhibitor Following Spinal Cord Injury on Recovery of Voiding Efficiency and Motor Function Observed by Regeneration in Spinal Cord

Scriptaid/exercise-induced lysine acetylation is another type of posttranslational modification occurring in titin

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