Combination of the ALCR/alcA ethanol switch and GAL4/VP16‐UAS enhancer trap system enables spatial and temporal control of transgene expression in <i>Arabidopsis</i>

Jia, Hong-Ti; Loock, Bram Van; Liao, Mingjun; Verbelen, Jean‐Pierre; Vissenberg, Kris

doi:10.1111/j.1467-7652.2007.00255.x

Cited by 15 publications

(8 citation statements)

References 35 publications

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“…thaliana [5, 10]. The potent inhibitory effect of ARR22 on the TCS also makes it an excellent tool for dissecting local phosphorelay-dependent effects when driven by tissue-specific inducible systems [11, 75, 76]. The combination of B-types with ARR22 and treatment specific stimuli should allow the identification of more direct target candidates of B-types, as shown here for WUS .…”

Section: Discussionmentioning

confidence: 99%

ARR22 overexpression can suppress plant Two-Component Regulatory Systems

et al. 2019

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In plants, several developmental processes are co-coordinated by cytokinins via phosphorylation dependent processes of the Two-Component System (TCS). An outstanding challenge is to track phosphorelay flow from cytokinin perception to its molecular outputs, of which gene activation plays a major role. To address this issue, a kinetic-based reporter system was expounded to track TCS phosphorelay activity in vivo that can distinguish between basal and cytokinin dependent effects of overexpressed TCS members. The TCS phosphorelay can be positively activated by cytokinin and inhibited by pharmaceuticals or naturally interfering components. In this case we took advantage of the phosphohistidine-phosphatase Arabidopsis Response Regulator (ARR) 22 and investigated its phosphocompetition with other TCS members in regulating promoters of ARR5 and WUS in Arabidopsis thaliana cell culture protoplasts. In congruency with the proposed function of ARR22, overexpression of ARR22 blocked the activation of all B-type ARRs in this study in a TCS dependent manner. Furthermore, this effect could not be mimicked by A-type response regulator overexpression or compensated by AHP overexpression. Compared to other reporter assays, ours mimicked effects previously observed only in transgenic plants for all of the TCS proteins studied, suggesting that it is possible to expose phosphocompetition. Thus, our approach can be used to investigate gene signaling networks involving the TCS by leveraging ARR22 as a TCS inhibitor along with B-type ARR overexpression.

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Section: Discussionmentioning

confidence: 99%

ARR22 overexpression can suppress plant Two-Component Regulatory Systems

et al. 2019

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“…The currently available systems for tissue- and cell-type-specific gene expression include either cloning desired promoters individually or using two-component systems [ 4 - 9 ]. In some of these two-component systems consisting of a transcription factor and a target promoter, treatment with inducers such as 17-β-estradiol [ 6 ] or ethanol [ 4 , 10 , 11 ] promotes the transcription factor binding to and activating the target promoter to allow for temporal control of gene activation in addition to the spatial control afforded by tissue-specific promoters. Although these systems have been instrumental in understanding spatial and temporal gene functions, they have the disadvantage of being unwieldy when studying tissue-specific gene rescue in higher order mutants.…”

Section: Introductionmentioning

confidence: 99%

Gateway-compatible tissue-specific vectors for plant transformation

2015

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BackgroundUnderstanding regulation of developmental events has increasingly required the use of tissue-specific expression of diverse genes affecting plant growth and environmental responses.FindingsTo allow for cloning of presumptive promoters with tissue-specific activities, we created two plant expression vectors with multiple cloning sites upstream of a Gateway cassette for expression of either untagged or YFP-tagged genes of interest. For fast and easy tissue-specific expression of desired genes, we further developed an initial set of Gateway-compatible tissue-specific gene expression vectors that allow for the expression of YFP-tagged or untagged proteins driven by the ALCOHOL DEHYDROGENASE1, CHLOROPHYLL A/B BINDING PROTEIN 1, COBRA LIKE1, EXPANSIN7, LATERAL ORGAN BOUNDARIES-DOMAIN 16, SCARECROW, UBIQUITIN10, and WOODEN LEG upstream regulatory regions.ConclusionsThese vectors provide an invaluable resource to the plant community, allowing for rapid generation of a variety of tissue-specific expression constructs.

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“…Another variant, GAL4VP16, is a chimera consisting of the GAL4 DNA‐binding domain fused to the transcriptional activation domain of the herpesvirus protein VP16. GAL4VP16 is 100‐fold more active than GAL4 in mammalian cells, (Sadowski et al, 1988), and it has been used for analyses of gene function in vertebrates (Grabher and Wittbrodt, 2004; Davison et al, 2007; Ogura et al, 2009), insects (Viktorinová and Wimmer, 2007; Schinko et al, 2010), and plants (Liang et al, 2006; Jia et al, 2007). A third GAL4 variant, GAL4NFκB, possesses the DNA‐binding region of GAL4 and a transcriptional activation domain of mouse nuclear factor‐κB (NF‐κB; Liu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

An efficient binary system for gene expression in the silkworm, Bombyx mori, using GAL4 variants

Kobayashi

Kojima

Uchino

et al. 2011

Arch Insect Biochem Physiol

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A binary gene expression system using the yeast GAL4 DNA-binding protein and the upstream activating sequence (UAS) of galactose-driven yeast genes is an established and powerful tool for the analysis of gene function. However, in the domesticated silkworm, Bombyx mori, this system has been limited in its utility by the relatively low transcriptional activation activity of GAL4 and by its toxicity. In this study, we investigated the potential of several established GAL4 variants (GAL4Δ, GAL4VP16, GAL4VPmad2, GAL4VPmad3, and GAL4NFκB) and of two new GAL4 variants, GAL4Rel and GAL4Relish, which contain the transcription-activating regions of the BmRel and BmRelish genes, respectively, to improve the utility of the GAL4/UAS system in B. mori. We generated constructs containing these GAL4 variants under the control of constitutive or inducible promoters and investigated their transcription-activating activity in cultured B. mori cells and embryos and in transgenic silkworms. GAL4VP16 and GAL4NFκB exhibited high transactivation activity but appeared to be toxic when used as transgenes under the control of a constitutive promoter. Similarly, GAL4VPmad2 and GAL4VPmad3 exhibited higher transactivation activity than GAL4, combined with strong toxicity. The transcription-activating activity of GAL4Δ was about twice that of GAL4. The two new GAL4 variants, GAL4Rel and GAL4Relish, were less active than GAL4. Using GAL4VP16 and GAL4NFκB constructs, we have developed a very efficient GAL4/UAS binary gene expression system for use in cultured B. mori cells and embryos and in transgenic silkworms.

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Combination of the ALCR/alcA ethanol switch and GAL4/VP16‐UAS enhancer trap system enables spatial and temporal control of transgene expression in Arabidopsis

Cited by 15 publications

References 35 publications

ARR22 overexpression can suppress plant Two-Component Regulatory Systems

ARR22 overexpression can suppress plant Two-Component Regulatory Systems

Gateway-compatible tissue-specific vectors for plant transformation

An efficient binary system for gene expression in the silkworm, Bombyx mori, using GAL4 variants

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