Combination of size exclusion and normal-phase partition modes in high performance liquid chromatography

Size exclusion chromatographic analysis of refuse-derived fuel for mycotoxins

“…Recently, Mori (8,9) has proposed that the separation mechanism is a function of the polarity of the solvent. By varying the solvent polarity, one can change the elution behavior from a reversed-phase to a normal-phase system.…”

New analytical techniques for mycotoxins in complex organic matrices

“…This change in mechanism is contrary to that proposed by Mori [66]. This is inconsistent with other work [67] where it was suggested that partitioning occurred in a wide range of modifier concentrations, and performance could be regener ated after exposure to 50% modifier. The data presented here indicate that partitioning probably occurs at modifier concentrations less than 40%.…”

“…The mech anism now involves sorption rather than partition, a distinction that is often overlooked. The preceding discus sion is in agreement with that in references [66] and [67].…”

“…References [66] and [67] use PS-DVB gels as a stationary phase. Styragel, a similar material, was used for this study.…”

“…Other work on solvent effects used alcohols as modifiers [66,67]. However, solubility parameter theory breaks down for strongly hydrogen-bonding solvents [69].…”