Combination of RNA sequencing and metabolite data to elucidate improved toxic compound tolerance and butanol fermentation of Clostridium acetobutylicum from wheat straw hydrolysate by supplying sodium sulfide

Jin, Yanling; Fang, Yang; Huang, Mengjun; Sun, Jiaolong; Huang, Yuhong; Gao, Xiaofeng; Li, Renqiang; He, Kai; Zhao, Hai

doi:10.1016/j.biortech.2015.08.139

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…4). Our results are consistent with results obtained by Jin et al, where they observed a down regulation of the metabolic flux towards the acid formation branch ("cold channel"), and an up-regulation of the metabolic flux toward the ABE formation branches ("hot channel"); and consequently improved C. acetobutylicum fermentation of a non-detoxified wheat straw hydrolysate supplemented with sodium sulfite [39].…”

Section: Evaluation Of Evolved Isolates Towards Acetic Acid and Hmfsupporting

confidence: 93%

Enhancing acetic acid and 5‐hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution

Alves

Zetty-Arenas

Demirci

et al. 2021

Process Biochemistry

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In this study, adaptive laboratory evolution (ALE) was applied to isolate four strains of Clostridium saccharoperbutylacetonicum able to grow in the presence of hemicellulosic hydrolysate inhibitors unsupported by the parental strain. Among them, isolate RAC-25 presented the best fermentative performance, producing 22.1 g/L of ABE and 16.7 g/L of butanol. Genome sequencing revealed a deletion in the arabinose transcriptional repressor gene (araR) and a mutation in the anti-sigma factor I that promoted a downregulation of sigI. Gene expression analysis indicated high expression of genes related to H +-pumps (ATP synthases), proline biosynthesis (gamma phosphate reductase) and chaperonins (Grol), suggesting an integrated mechanism that is probably coordinated by the repression of sigI. Therefore, in addition to highlighting the power of ALE for selecting robust strains, our results suggest that sigI and araR may be interesting gene targets for increased tolerance toward inhibitor compounds relevant for lignocellulosic biofuels production.

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Section: Evaluation Of Evolved Isolates Towards Acetic Acid and Hmfsupporting

confidence: 93%

Enhancing acetic acid and 5‐hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution

Alves

Zetty-Arenas

Demirci

et al. 2021

Process Biochemistry

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“…corp., CA) using an Aminex HPX-87-Pb column (Bio-Rad, Hercules, USA) at 80°C and flow rate of 0.6 ml/min with distilled water as eluent. Nitrogen gas was used as carrier at the pressure of 2.8 Bar and draft temperature was 105°C for the ELSD detector [11,19]. Protein content was determined by the Kjeldahl method [18].…”

Section: Characterization Of the Sprmentioning

confidence: 99%

“…The xylanase (Multifect, DuPont TM Genencor ® Science) had a declared activity of 8,000 GXU/mL, 47 mg protein/ml. GXU is based on the release of Remazol Brilliant Blue-dyed birch wood xylan at pH 4.5 and 30℃ in 10 min, using a xylanase reference standard [19].…”

Section: Enzymatic Hydrolysis Of the Sprmentioning

confidence: 99%

“…Yeast extract was supplied as an extra nitrogen source in corn fiber hydrolysate [12]. KH 2 PO 4 and MgSO 4 were added to wheat straw hydrolysate to ensure the successful butanol fermentation [19]. In order to know whether nitrogen source and mineral were the restriction factor for cell growth and butanol production besides hydrolysis inhibitors, nutrition supplement experiments were carried out.…”

Section: Effect Of Additional Nutrients On Abe Fermentationmentioning

confidence: 99%

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Waste-to-energy: biobutanol production from cellulosic residue of sweet potato by Clostridia acetobutylicum

Jin¹,

Zhang²,

Yi³

et al. 2021

Environmental Engineering Research

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Biobutanol has been emerging as a renewable replacement to overcome fossil fuel limit due to its attractive qualities. The waste resources utilization is an economically feasible and eco-friendly way to produce biobutanol. In this work, the potential of butanol fermentation with sweet potato residue (SPR) obtained from ethanol fermentation was evaluated. To assist sugar release for butanol production by Clostridia acetobutylicum, a pretreatment approach with dilute sulfuric acid was established via response surface methodology. The maximum butanol concentration of 3.77 g/L was obtained at the optimal conditions of pretreatment at 1% (v/v) dilute sulfuric acid, and 115℃ for 30 min. Detoxifying the hydrolysate with XAD-4 resin significantly enhanced butanol fermentation performance, while adding nutritional supplements had no significant influence. The stability of the fermentation process was verified by the 2 L-scale of fermentation, resulting in 7.96 g/L of butanol, 13.42 g/L h of Acetone-Butanol-Ethanol (ABE), and 0.34 g/g of ABE yield, respectively. This study demonstrates a bio-refinery strategy of conversion from waste biomass to energy, confirms the feasibility of utilizing the cost saving SPR as feedstock to produce butanol through fermentation, and provides a novel approach for minimization of environmental pollution.

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“…RNA-Seq is considered as one of the most frequently used methods for transcriptome analysis and gene expression pro ling [13], it has many advantages compared with other gene expression pro ling technologies, such as allowing a comprehensive assay that does not require probes for targets to be speci ed in advance [14]. In recent years, the highthroughput RNA-Seq technique has emerged as a useful tool for transcriptome analysis and exploring unknown genes [15].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus

Lin

Zou

Zhou

et al. 2019

BioMed Research International

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Caterpillar fungus is a well-known fungal Chinese medicine. To reveal molecular changes during early and late stages of adenosine biosynthesis, transcriptome analysis was performed with the anamorph strain of caterpillar fungus. A total of 2,764 differentially expressed genes (DEGs) were identified (p≤0.05, |log2 Ratio| ≥ 1), of which 1,737 were up-regulated and 1,027 were down-regulated. Gene expression profiling on 4–10 d revealed a distinct shift in expression of the purine metabolism pathway. Differential expression of 17 selected DEGs which involved in purine metabolism (map00230) were validated by qPCR, and the expression trends were consistent with the RNA-Seq results. Subsequently, the predicted adenosine biosynthesis pathway combined with qPCR and gene expression data of RNA-Seq indicated that the increased adenosine accumulation is a result of down-regulation of ndk, ADK, and APRT genes combined with up-regulation of AK gene. This study will be valuable for understanding the molecular mechanisms of the adenosine biosynthesis in caterpillar fungus.

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Combination of RNA sequencing and metabolite data to elucidate improved toxic compound tolerance and butanol fermentation of Clostridium acetobutylicum from wheat straw hydrolysate by supplying sodium sulfide

Cited by 11 publications

References 33 publications

Enhancing acetic acid and 5‐hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution

Enhancing acetic acid and 5‐hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution

Waste-to-energy: biobutanol production from cellulosic residue of sweet potato by Clostridia acetobutylicum

Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus

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