Combination of phenylboronic acid and oligocytosine for selective and specific detection of lead(ii) by lateral flow test strip

Berlina, Anna N.; Komova, Nadezhda S.; Zherdev, Anatoly V.; Dzantiev, Boris B.

doi:10.1016/j.aca.2021.338318

Cited by 13 publications

(4 citation statements)

References 71 publications

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“…The CNPC15 membrane is often used for analysis in various media. Previously, we applied this membrane for LFIA of low molecular weight analytes–antibiotic chloramphenicol and Sudan I dye [ 53 , 54 ], and heavy metals such as lead [ 55 ]. However, in the case of DBP, a weak coloration was formed during the interaction in LFIA and the band in the test zone looked pale ( Figure 5 , columns 1 and 2).…”

Section: Resultsmentioning

confidence: 99%

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

et al. 2022

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The use of a large amount of toxic synthetic materials leads to an increase in the pollution of environmental objects. Phthalates are compounds structurally related to esters of phthalic acid that are widely used in the manufacturing of synthetic packaging materials as plasticizers. Their danger is conditioned by leaching into the environment and penetrating into living organisms with negative consequences and effects on various organs and tissues. This work presents the first development of lateral flow immunoassay to detect dibutyl phthalate, one of the most common representatives of the phthalates group. To form a test zone, a hapten–protein conjugate was synthesized, and gold nanoparticles conjugated with antibodies to dibutyl phthalate were used as a detecting conjugate. The work includes the preparation of immunoreagents, selectivity investigation, and the study of the characteristics of the medium providing a reliable optical signal. Under the selected conditions for the analysis, the detection limit was 33.4 ng/mL, and the working range of the determined concentrations was from 42.4 to 1500 ng/mL. Time of the assay–15 min. The developed technique was successfully applied to detect dibutyl phthalate in natural waters with recovery rates from 75 to 115%.

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Section: Resultsmentioning

confidence: 99%

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

et al. 2022

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“…Although multiple bacteria can be distinguished by setting multiple antibodies on the different test lines (T-lines), the acquisition process is often relatively complicated and costly. At present, phenylboronic acid and its derivatives as the recognition units was an alternative approach for bacterial capture and detection, as they can covalently interact with cis-diol containing glycoproteins on the surface of bacteria, forming stable cyclic esters. , Wu et al successfully applied phenylboric acid to LFIA bacteria detection, which was meaningful for the progress of antibodies (Abs)-dependent LFIA . Despite this success, conventional colorimetric (CM)-based LFIA only by visual observation suffers from having low sensitivity and difficulty in quantitative detection.…”

Section: Introductionmentioning

confidence: 99%

Urchin-Shaped Au–Ag@Pt Sensor Integrated Lateral Flow Immunoassay for Multimodal Detection and Specific Discrimination of Clinical Multiple Bacterial Infections

et al. 2023

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A new lateral flow immunoassay strip (LFIA) combining sensitive detection and identification of multiple bacteria remains a huge challenge. In this study, we first developed multifunctional urchin-shaped Au−Ag@Pt nanoparticles (UAA@P NPs) with a unique combination of colorimetric-SERS-photothermal-catalytic (CM/SERS/PT/CL) properties and integrated them with LFIA for multiplexed detection and specific discrimination of pathogenic bacteria in blood samples. Unlike the conventional LFIA that relied on antibody (Ab), this novel LFIA introduced 4-mercaptophenylboronic acid (4-MPBA) as an ideal Ab replacer that was functionalized on UAA@P NPs (UAA@P/M NPs) with outstanding binding and enrichment capacities toward bacteria. Taking Staphylococcus aureus (S. aureus) as model bacteria, the limit of detection (LOD) was 3 CFU/mL for SERS-LFIA, 27 CFU/mL for PT-LFIA, and 18 CFU/mL for CL-LFIA, three of which were over 330-fold, 37-fold, and 55-fold more sensitive than ordinary visual CM-LFIA, respectively. Besides, this SERS-LFIA is capable of identifying three types of bacterial spiked blood samples (E. coli, S. aureus, and P. aeruginosa) effectively according to specific bacterial Raman "fingerprints" by partial least-squaresdiscriminant analysis (PLS-DA). More importantly, this LFIA was successfully applied to blood samples with satisfactory recoveries from 90.3% to 108.8% and capable of identifying the infected patients (N = 4) from healthy subjects (N = 2) with great accuracy. Overall, the multimodal LFIA incorporates bacteria discrimination and quantitative detection, offering an avenue for early warning and diagnosis of bacterial infection.

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“…Lateral flow assay (LFA) based on gold nanoparticles (AuNPs) is a simple and low-cost analytical device for the rapid analysis of target molecules on site. − As compared with other point-of-care (POC) detection methods, LFA is distinctive as it produces a control line during testing, indicating the correct operation of this POC method. , Over the past decades, a wide variety of LFA strategies have been developed to test many different molecules, including proteins, nucleic acids, small molecules, metal ions, reactive small species, and enzymes. − Currently, several LFA tool kits are available commercially for the rapid testing of pregnancy, infectious diseases, and food safety. − Depending on the type of target molecules, LFA detection can be carried out in either the sandwich or the competitive format. , In the presence of a macromolecule target (e.g., proteins or nucleic acids), LFA tests generate a “Signal-ON” test line when an antibody-analyte-antibody sandwich complex is formed on the membrane. In contrast, small molecules usually lack the multiple binding epitopes required to simultaneously bind with two antibody recognition units.…”

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confidence: 99%

Affinity-Switchable Interaction of Biotin and Streptavidin for the Signal-ON Detection of Small Molecules

Chung,

Liu,

Jian

et al. 2023

ACS Sens.

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Lateral flow assay (LFA) based on gold nanoparticles (AuNPs) is a widely used analytical device for the rapid analysis of environmental hazards and biomarkers. Typically, a sandwich-type format is used for macromolecule detection, in which the appearance of a red test line indicates a positive result (Signal-ON). In contrast, small molecule detection usually relies on a competitive assay, where the absence of a test line indicates positive testing (Signal-OFF). However, such a “Signal-OFF” reading is usually detected within a narrower dynamic range and tends to generate false-negative signals at a low concentration. Moreover, inconsistent readings between macromolecule and small molecule testing might lead to misinterpretation when used by nonskilled individuals. Herein, we report a “Signal-ON” small molecule competitive assay based on the sterically modulated affinity-switchable interaction of biotin and streptavidin. In the absence of a small molecule target, a large steric hindrance can be imposed on the biotin to prevent interaction with streptavidin. However, in the presence of the small molecule target, this steric effect is removed, allowing the biotin to bind to streptavidin and generate the desired test line. In this article, we demonstrate the selective detection of two small molecule drugs, sulfonamides and trimethoprim, using this simple and modular affinity-switchable lateral flow assay (ASLFA). We believe that this affinity-switchable approach can also be adapted in drug discovery and clinical diagnosis, where the competitive assay format is always used for the rapid analysis of small molecules.

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Combination of phenylboronic acid and oligocytosine for selective and specific detection of lead(ii) by lateral flow test strip

Cited by 13 publications

References 71 publications

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

Urchin-Shaped Au–Ag@Pt Sensor Integrated Lateral Flow Immunoassay for Multimodal Detection and Specific Discrimination of Clinical Multiple Bacterial Infections

Affinity-Switchable Interaction of Biotin and Streptavidin for the Signal-ON Detection of Small Molecules

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