Combination of microfluidic chips and biosensing for the enrichment of circulating tumor cells

Shi, Jiaju; Zhao, Chunqin; Shen, Meiqi; Chen, Zichao; Liu, Jinhua; Zhang, Shusheng; Zhang, Zhen

doi:10.1016/j.bios.2022.114025

Cited by 16 publications

(9 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our system has lower capture efficiency than a pervious microfluidic device (99.5% at 0.6 ml/h), but it is simpler and cheaper 50 . Over recent years, microfluidic chips have gained considerable attention for cell www.nature.com/scientificreports/ isolation 51,52 . In microfluidics, capture efficiency and cell purity can often be achieved more efficiently than with nanomaterials; however, bubble formation or cell entrapment can restrict their clinical use.…”

Section: Resultsmentioning

confidence: 99%

Facile preparation of a cost-effective platform based on ZnFe2O4 nanomaterials for electrochemical cell detection

Vajhadin

Mazloum‐Ardakani

Hemati

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Circulating tumor cells (CTCs) are important tumor markers that indicate early metastasis, tumor recurrence, and treatment efficacy. To identify and separate these cells from the blood, new nanomaterials need to be developed. The present study explored the potential application of ZnFe2O4 magnetic nanoparticles in capturing CTCs with cell surface markers. Folic acid was coupled to l-cysteine-capped ZnFe2O4 nanoparticles (ZC) to provide binding sites on ZnFe2O4 nanoparticles for the recognition of folate bioreceptors, which are highly expressed in MCF-7 breast cancer cells. The cytotoxicity of ZnFe2O4 nanoparticles and ZC against MCF-7 was analyzed with the MTT assay. After 24 h of incubation, there were IC50 values of 702.6 and 805.5 µg/mL for ZnFe2O4 and ZC, respectively. However, after 48 h of incubation, IC50 values of ZnFe2O4 and ZC were reduced to 267.3 and 389.7 µg/mL, respectively. The cell quantification was conducted with magnetically collected cells placed on a glassy carbon electrode, and the differential pulse voltammetry (DPV) responses were analyzed. This cost-effective ZnFe2O4-based biosensing platform allowed cancer cell detection with a limit of detection of 3 cells/mL, ranging from 25 to 104 cells/mL. In future, these functionalized zinc ferrites may be used in electrochemical cell detection and targeted cancer therapy.

show abstract

Section: Resultsmentioning

confidence: 99%

Facile preparation of a cost-effective platform based on ZnFe2O4 nanomaterials for electrochemical cell detection

Vajhadin

Mazloum‐Ardakani

Hemati

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…As is well-known, CTCs are malignant cells found in the peripheral blood circulatory system originating from solid tumors, which play a vital role in cancer metastasis. 84,85 It is reported that about 90% of cancerrelated deaths are derived from the metastatic spread of tumor cells. 86,87 However, isolating CTCs from billions of background cells is extremely challenging.…”

Section: ■ Application Of Extracted Cell Membrane For Biosensingmentioning

confidence: 99%

Engineering Cell Membranes: From Extraction Strategies to Emerging Biosensing Applications

Tan,

Zhu,

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

“…Due to the high surface area volume ratio, compatible capture and release ability, nanomaterials provide a high collision probability for antibodies and CTCs ( Park et al, 2017 ; Wu C. et al, 2019 ; Shi et al, 2022 ; Yi et al, 2022 ; Liu et al, 2022 ; Liu J. et al, 2021 ), which can help improve the sensitivity and specificity of capture, and have attracted extensive attention in the separation of CTCs ( Wongkaew et al, 2018 ; Wu and Qu, 2015 ; Zhu G. et al, 2021 ; Naskar et al, 2020 ; Chandankere et al, 2020 ). So far, carbon nanotubes (CNTs) ( Cho H. et al, 2018 ; Zhang P. et al, 2019 ; Poudineh et al, 2017 ), graphene oxide (GO) ( Chen et al, 2012 ; Yoon et al, 2013 ; Yu X. et al, 2013 ; Yoon et al, 2016 ), gold nanoparticles (AuNP) ( Park et al, 2017 ), nanocolumns ( Lin et al, 2014 ; Shi et al, 2022 ) and TiO 2 nanofibers ( Zhang et al, 2012 ) have been widely used in affinity group capture methods ( Zhao et al, 2016 ; Cai et al, 2017 ; Wongkaew et al, 2018 ). NanoVelcro CTCs chip is a representative nanostructure microfluidic platform.…”

Section: Microfluidic Technologies For Ctcs Separationmentioning

confidence: 99%

Application of Microfluidics in Detection of Circulating Tumor Cells

Wang

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Tumor metastasis is one of the main causes of cancer incidence and death worldwide. In the process of tumor metastasis, the isolation and analysis of circulating tumor cells (CTCs) plays a crucial role in the early diagnosis and prognosis of cancer patients. Due to the rarity and inherent heterogeneity of CTCs, there is an urgent need for reliable CTCs separation and detection methods in order to obtain valuable information on tumor metastasis and progression from CTCs. Microfluidic technology is increasingly used in various studies of CTCs separation, identification and characterization because of its unique advantages, such as low cost, simple operation, less reagent consumption, miniaturization of the system, rapid detection and accurate control. This paper reviews the research progress of microfluidic technology in CTCs separation and detection in recent years, as well as the potential clinical application of CTCs, looks forward to the application prospect of microfluidic technology in the treatment of tumor metastasis, and briefly discusses the development prospect of microfluidic biosensor.

show abstract

Combination of microfluidic chips and biosensing for the enrichment of circulating tumor cells

Cited by 16 publications

References 104 publications

Facile preparation of a cost-effective platform based on ZnFe2O4 nanomaterials for electrochemical cell detection

Facile preparation of a cost-effective platform based on ZnFe2O4 nanomaterials for electrochemical cell detection

Engineering Cell Membranes: From Extraction Strategies to Emerging Biosensing Applications

Application of Microfluidics in Detection of Circulating Tumor Cells

Contact Info

Product

Resources

About