Combination of mass spectrometry and site‐specific NMR isotope analyses in the characterisation of amino acids

Vallet, Claude; Arendt, Margaretha; Mabon, Françoise; Naulet, N.; Martin, G. J.

doi:10.1002/jsfa.2740560207

Cited by 10 publications

(9 citation statements)

References 31 publications

(24 reference statements)

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“…Taking into account the origin of the atoms in both arginine and ornithine and the symmetry of putrescine, the methylene sites 8, 12 and 10, 11 of the pyrrolidine ring of nicotine are supposed to arise from the C3 and C4 methylenic groups of glutamic acid (50% each). In this respect, an investigation of the distribution of deuterium in glutamic acid by the SNIF-NMR method (Vallet et al 1991) has shown that relatively wide ranges of variation are exhibited by the site-specific hydrogen isotope ratios. Sites 6 and 5,7 of nicotine arise from C2 or C4 atoms of ornithine or arginine.…”

Section: Deuterium Distribution In the Nicotine Moleculementioning

confidence: 99%

“…In this respect, an investigation of the distribution of deuterium in glutamic acid by the SNIF-NMR method (Vallet et al 1991) has shown that relatively wide ranges of variation are exhibited by the site-specific hydrogen isotope ratios. Sites 6 and 5,7 of nicotine arise from C2 or C4 atoms of ornithine or arginine.…”

Section: Deuterium Distribution In the Nicotine Moleculementioning

confidence: 99%

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Multi‐element and multi‐site isotopic analysis of nicotine from tobacco leaves

Jamin

Naulet

Martin

1997

Plant Cell & Environment

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The carbon, nitrogen and hydrogen stable-isotope contents of nicotine extracted from tobacco leaves were determined by Isotope Ratio Mass Spectrometry (overall ^H, ^^C and^N contents) and by the SNIF-NMR method (site-specific deuterium content). In addition, nicotine was chemically degraded into nicotinic acid so that the intramolecular distribution of carbon and nitrogen isotopes could be studied. A prerequisite for reliable measurements is the use of experimental procedures free of isotopic fractionation. Therefore, it was ensured that isotopic integrity was maintained throughout extraction, purification and chemical degradation steps. The multi-element and multi-site information provided is tentatively interpreted in terms of biochemical isotopic effects on intramolecular distribution patterns and of the infiuence of environmental factors on nicotine isotopic parameters.

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Section: Deuterium Distribution In the Nicotine Moleculementioning

confidence: 99%

Section: Deuterium Distribution In the Nicotine Moleculementioning

confidence: 99%

Multi‐element and multi‐site isotopic analysis of nicotine from tobacco leaves

Jamin

Naulet

Martin

1997

Plant Cell & Environment

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“…Approximately 10 mg of freeze-dried leaf material was weighed into tin capsules, which were sealed and placed inside a zero-blank autosampler attached to a Costech Elemental Analyzer ECS4010 (Costech International). Samples were analyzed according to Vallet et al (1991).…”

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confidence: 99%

TILLING Mutants of Lotus japonicus Reveal That Nitrogen Assimilation and Fixation Can Occur in the Absence of Nodule-Enhanced Sucrose Synthase

Horst

Welham

Kelly³

et al. 2007

Plant Physiology

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In all plant species studied to date, sucrose synthase occurs as multiple isoforms. The specific functions of the different isoforms are for the most part not clear. Six isoforms of sucrose synthase have been identified in the model legume Lotus japonicus, the same number as in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa). The genes encoding these isoforms are differentially expressed in all plant organs examined, although one, LjSUS4, is only expressed in flowers. LjSUS1 is the most highly expressed in all plant organs tested, except root nodules, where LjSUS3 accounts for more than 60% of the total SUS transcripts. One gene, LjSUS2, produces two transcripts due to alternative splicing, a feature not observed in other species to date. We have isolated plants carrying ethyl methanesulfonate-induced mutations in several SUS genes by targeting-induced local lesions in genomes reverse genetics and examined the effect of null alleles of two genes, LjSUS1 and LjSUS3, on nodule function. No differences were observed between the mutants and wild-type plants under glasshouse conditions, but there was evidence for a nitrogenstarvation phenotype in the sus3-1 mutant and severe impairment of growth in the sus1-1/sus3-1 double mutant under specific environmental conditions. Nodules of sus3-1 mutant plants retained a capacity for nitrogen fixation under all conditions. Thus, nitrogen fixation can occur in L. japonicus nodules even in the absence of LjSUS3 (the major nodule-induced isoform of SUS), so LjSUS1 must also contribute to the maintenance of nitrogen assimilation.

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“…NMR/MS experiments on the analysis of site-specific natural isotope fractionation after combustion or preparation in a glove box under anhydrous nitrogen atmosphere are described [348]. This "multi-isotopic approach" is proposed for origin identification of plant and other samples.…”

Section: Analytical Systemsmentioning

confidence: 99%

“…The NMR technique was used in humus research [ 120, 336, 3371, fertilizer transformation experiments [3 11, ammonia fixation studies [337], metabolic studies in vivo [7,8,105,3601, monitoring routes for nitrogen assimilation [63], and studying site-specific natural isotope fractionation of amino acids [348].…”

Section: "N Measurementsmentioning

confidence: 99%

Advances in nitrogen-15 use for environmental studies in the soil-plant system

Faust

1993

Isotopenpraxis Isotopes in Environmental and Health Studies

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A review is being presented of the latcst results in the use of nitrogen-15 in the soil-plant system. The literature covers the period from 1991 to early April 1993. The increase in environmentally related experiments shows that the stable nitrogen isotope technique, applied either as tracer or as the natural isotope tracing approach, is well suited particularly to ecosystem studies. This trend is supported by convenient analytical systems. An important challenge to scientists in the field of environmental research is to contrive ways and means of permitting the plants to utilize the available nitrogen in the soil more efficiently. Examples of research areas needing more attention are pointed out.

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Combination of mass spectrometry and site‐specific NMR isotope analyses in the characterisation of amino acids

Abstract: Experimental strategies have been developed which make amino acid probes accessible to the N M R study of site-specijir natural isotope fractionation.A multi-isotope approach, based on combined mass spectrometry determination of hydrogen-, carbon-and nitrogen-overall

Cited by 10 publications

References 31 publications

Multi‐element and multi‐site isotopic analysis of nicotine from tobacco leaves

Multi‐element and multi‐site isotopic analysis of nicotine from tobacco leaves

TILLING Mutants of Lotus japonicus Reveal That Nitrogen Assimilation and Fixation Can Occur in the Absence of Nodule-Enhanced Sucrose Synthase

Advances in nitrogen-15 use for environmental studies in the soil-plant system

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