Combination of hyaluronic acid and PLGA particles as hybrid systems for viscosupplementation in osteoarthritis

Mota, Ana Henriques; Direito, Rosa; Carrasco, Marta P.; Rijo, Patrícia; Ascensão, Lia; Viana, Ana S.; Rocha, José Antonio; Figueira, Maria Eduardo; Rodrigues, Maria João; Custódio, Luísa; Kuplennik, Nataliya; Sosnik, Alejandro; Almeida, António J.; Gaspar, Maria Manuela; Reis, Catarina Pinto

doi:10.1016/j.ijpharm.2019.01.017

Cited by 24 publications

(28 citation statements)

References 33 publications

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“…To encapsulate the extract, two different NPs containing oleic acid as a capping agent to control NPs growth and aggregation, were developed, according to previous studies [12,13].…”

Section: Preparation Of Extract-loaded Nanoparticlesmentioning

confidence: 99%

“…PLGA NPs were prepared by emulsification/solvent diffusion [5,13,14]. In brief, 50 mg PLGA was dissolved in a mixture acetone: ethanol (8:2, v/v) was added into an aqueous phase consisting of 0.1% PF127 and oleic acid (11 µL) under magnetic stirring (400 rpm), during 2 h [5,15].…”

Section: Preparation Of Extract-loaded Nanoparticlesmentioning

confidence: 99%

“…For the atomic force microscopy (AFM) analysis, the samples were prepared by centrifugation using 7378× g (Sigma Laborzentrifugen, Osterode am Harz, Germany) for 5 and 10 min, for empty and extract-loaded NPs, respectively, followed by resuspension in water (for half of the previous volume) [13]. AFM uses diluted samples (1:2, v/v) without any pre-treatment.…”

Section: Morphological Characterizationmentioning

confidence: 99%

“…AFM uses diluted samples (1:2, v/v) without any pre-treatment. A drop of ≈40 µL of the sample was placed on a freshly cleaved mica surface to allow the absortion for around 30 min [13]. Afterwards, the samples were dried under a stream of N 2 and analyzed in intermittent mode (Nanoscope V, Multimode 8 HR Microscope, produced by Bruker, Billerica, MA, USA) [13].…”

Section: Morphological Characterizationmentioning

confidence: 99%

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Further Evidence of Possible Therapeutic Uses of Sambucus nigra L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations

et al. 2020

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Sambucus nigra L. is widely used in traditional medicine with different applications. However, confirmative studies are strongly required. This study aimed to assess the biological activities of the S. nigra flower’s extract encapsulated into two different types of nanoparticles for optimizing its properties and producing further evidence of its potential therapeutic uses. Different nanoparticles (poly(lactide-co-glycolide, PLGA) and poly-Ɛ-caprolactone (PCL), both with oleic acid, were prepared by emulsification/solvent diffusion and solvent-displacement methods, respectively. Oleic acid was used as a capping agent. After the nanoparticles’ preparation, they were characterized and the biological activities were studied in terms of collagenase, in vitro and in vivo anti-inflammatory, and in vitro cell viability. Rutin and naringenin were found to be the major phenolic compounds in the studied extract. The encapsulation efficiency was higher than 76% and revealed to have an impact on the release of the extract, mainly for the PLGA. Moreover, biochemical and histopathological analyses confirmed that the extract-loaded PLGA-based nanoparticles displayed the highest anti-inflammatory activity. In addition to supporting the previously reported evidence of potential therapeutic uses of S. nigra, these results could draw the pharmaceutical industry’s interest to the novelty of the nanoproducts.

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“…To encapsulate the extract, two different NPs containing oleic acid as a capping agent to control NPs growth and aggregation, were developed, according to previous studies [12,13].…”

Section: Preparation Of Extract-loaded Nanoparticlesmentioning

confidence: 99%

Section: Preparation Of Extract-loaded Nanoparticlesmentioning

confidence: 99%

Section: Morphological Characterizationmentioning

confidence: 99%

Section: Morphological Characterizationmentioning

confidence: 99%

See 2 more Smart Citations

Further Evidence of Possible Therapeutic Uses of Sambucus nigra L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations

et al. 2020

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“…Mota et al have prepared PLGA-NPs with oleic acid and HA on their surface, showing a similar anti-inflammatory activity to diclofenac and higher than HA free solution, in rats implanted with cotton pellets subcutaneously to promote granuloma and inflammation [120]. HA and chitosan (HA/CS) NPs have also been developed to deliver into the joints the gene CrmA, cytokine response modifier A, a protease inhibitor that binds and blocks caspase-1 enzyme, which is responsible for the generation of IL-1β in OA.…”

Section: Hyaluronic Acid-based Polymeric Nanoparticlesmentioning

confidence: 99%

Therapeutic Manipulation of Macrophages Using Nanotechnological Approaches for the Treatment of Osteoarthritis

et al. 2020

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Osteoarthritis (OA) is the most common joint pathology causing severe pain and disability. Macrophages play a central role in the pathogenesis of OA. In the joint microenvironment, macrophages with an M1-like pro-inflammatory phenotype induce chronic inflammation and joint destruction, and they have been correlated with the development and progression of the disease, while the M2-like anti-inflammatory macrophages support the recovery of the disease, promoting tissue repair and the resolution of inflammation. Nowadays, the treatment of OA in the clinic relies on systemic and/or intra-articular administration of anti-inflammatory and pain relief drugs, as well as surgical interventions for the severe cases (i.e., meniscectomy). The disadvantages of the pharmacological therapy are related to the chronic nature of the disease, requiring prolonged treatments, and to the particular location of the pathology in joint tissues, which are separated anatomical compartments with difficult access for the drugs. To overcome these challenges, nanotechnological approaches have been investigated to improve the delivery of drugs toward macrophages into the diseased joint. This strategy may offer advantages by reducing off-target toxicities and improving long-term therapeutic efficacy. In this review, we describe the nanomaterial-based approaches designed so far to directly or indirectly manipulate macrophages for the treatment of osteoarthritis.

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Engineering of Viscosupplement Biomaterials for Treatment of Osteoarthritis: A Comprehensive Review

et al. 2022

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Osteoarthritis (OA) is a progressive degenerative disease that causes severe pain and functional limitation, especially during locomotion. It is the most common arthritis type that damages the surface of articular cartilage until the underlying bone. In the past decade, the scientific community has made a considerable effort to improve or discover therapeutical products used as a form of conservative treatment capable of restoring the damaged articular tissue, avoiding, as far as possible, the use of surgical practices. The most common and direct nonoperative application available for OA treatment is the viscosupplementation (VS) procedure that demonstrates a safe, effective method and is less painful for the patients. The most recent works dealing with the design, development, and validation of viscosupplement products in preclinical and clinical trials for OA treatment are overviewed herein. In general, despite the development of new products, hyaluronic acid continues to be among the most reported intra‐articular viscosupplement products used in clinical trials, typically used as an isolated product or conjugated with other biologicals or drugs, such as platelet‐rich plasma and corticosteroids (CS). However, this issue is still demanding innovation. Approaches comprising new biomaterials as VS products, with intrinsic bioactivity, economical, and environmental friendliness, are required.

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Combination of hyaluronic acid and PLGA particles as hybrid systems for viscosupplementation in osteoarthritis

Cited by 24 publications

References 33 publications

Further Evidence of Possible Therapeutic Uses of Sambucus nigra L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations

Further Evidence of Possible Therapeutic Uses of Sambucus nigra L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations

Therapeutic Manipulation of Macrophages Using Nanotechnological Approaches for the Treatment of Osteoarthritis

Engineering of Viscosupplement Biomaterials for Treatment of Osteoarthritis: A Comprehensive Review

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