Combination of fluconazole with silver nanoparticles produced by<i>Fusarium oxysporum</i>improves antifungal effect against planktonic cells and biofilm of drug-resistant<i>Candida albicans</i>

Longhi, Carline; Santos, Jussevania Pereira; Morey, Alexandre Tadachi; Marcato, Priscyla D.; Durán, Nélson; Pinge‐Filho, Phileno; Nakazato, Gerson; Yamada‐Ogatta, Sueli Fumie; Yamauchi, Lucy Megumi

doi:10.1093/mmy/myv036

Cited by 68 publications

(55 citation statements)

References 18 publications

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“…(Casa et al, 2015;Tang et al, 2015;Tang et al, 2014). Similar results were observed with itraconazole nanoparticle (Qiu et al, 2015), fluconazole nanoparticles (Longhi et al, 2015), and miconaloze (Kumar and Poornachandra, 2015). Nanotechnology was also used for new natural antifungal candidates.…”

Section: The Development Of Antifungal Natural Products Using Nanoparsupporting

confidence: 72%

Searching new antifungals: The use of in vitro and in vivo methods for evaluation of natural compounds

Scorzoni

Sangalli-Leite

Singulani

et al. 2016

Journal of Microbiological Methods

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In the last decades, the increased number of immunocompromised patients has led to the emergence of many forms of fungal infections. Furthermore, there are a restricted arsenal of antifungals available and an increase in the development of resistance to antifungal drugs. Because of these disadvantages, the search for new antifungal agents in natural sources has increased. The development of these new antifungal drugs involves various steps and methodologies. The evaluation of the in vitro antifungal activity and cytotoxicity are the first steps in the screening. There is also the possibility of antifungal combinations to improve the therapy and reduce toxicity. Despite that, the application of the new antifungal candidate could be used in association with photodynamic therapy or using nanotechnology as an ally. In vivo tests can be performed to evaluate the efficacy and toxicity using conventional and alternative animal models. In this work, we review the methods available for the evaluation of the antifungal activity and safety of natural products, as well as the recent advances of new technology in the application of natural products for antifungal therapy.

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Section: The Development Of Antifungal Natural Products Using Nanoparsupporting

confidence: 72%

Searching new antifungals: The use of in vitro and in vivo methods for evaluation of natural compounds

Scorzoni

Sangalli-Leite

Singulani

et al. 2016

Journal of Microbiological Methods

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“…AgNPs have been reported to kill microbial calls via ROS enhancement, DNA and nuclear damage (Hwang et al ; Lara et al ; Sanyasi et al ). This dual killing mechanism exerted by both AgNPs and drugs eradicate C. albicans biofilms at very low concentration (Longhi et al ). Similar observations were also made by Lara et al () while studying the effect of AgNPs on biofilm ultrastructure.…”

Section: Discussionmentioning

confidence: 99%

Acinetobacter sp. mediated synthesis of AgNPs, its optimization, characterization and synergistic antifungal activity against C. albicans

Nadhe

Singh²,

Wadhwani

et al. 2019

J of Applied Microbiology

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Aims To synthesize silver nanoparticles (AgNPs) with cell free extract of Acinetobacter sp. and evaluate antifungal activity against planktonic and biofilm of Candida. Also, to study mechanism of antifungal action of AgNPs. Methods and Result Acinetobacter spp were screened for synthesis of AgNPs. Physio‐chemical parameters were optimized to obtained monodispersed nanoparticles. Optimized nanoparticles were characterized using spectroscopic, microscopic and diffraction techniques. Antifungal and biofilm disruption activity of AgNPs (10 ± 5 nm) were investigated against C. albicans. Mechanism of antifungal activity of nanosilver was deduced by growth curve, reactive oxygen species generation, thiol interaction and microscopic analysis. Acinetobacter sp. GWRFH 45 gave maximum synthesis of AgNPs. At optimized condition monodispersed, spherical nanoparticles were obtained which were crystalline with negative surface charge. AgNPs exhibited antifungal activity against planktonic cells and biofilm of Candida. AgNPs showed synergistic effect with amphotericin B as well as fluconazole against biofilm disruption. AgNPs were found to affect growth of Candida, generate reactive oxygen species and disrupt cellular morphology. Conclusions Cell free extract of A. calcoaceticus GWRFH 45 has ability to synthesize AgNPs. AgNPs alone and in combination with drugs have potential to inhibit C. albicans. Significance and Impact of the Study This is the first report of bacteriogenic AgNPs used in combination with antifungal drugs against Candida.

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“…For Ag/AgCl nanoparticles; generally, it can be conclude that MIC values of 2-7 μg/mL were obtained by treating E. coli with 20-70 nm nanoparticle sizes in different strains including ATCC 8739 and ATCC 25922. In the case of S. aureus, MIC values in the range of 1.9-3.4 μg/mL were found for 20 nm of Ag/AgCl nanoparticles (ATCC 06538 and 05, 923) Longhi et al 2016;Ballottin et al 2016). Table 4 reveals that higher values of MIC are observed for nanoparticles with higher size values, as measured by DLS technique.…”

Section: Attempt To Correlate the Antibacterial Activities Among The mentioning

confidence: 99%

Antimicrobial activity of biogenic silver nanoparticles, and silver chloride nanoparticles: an overview and comments

Durán

Nakazato

Seabra

2016

Appl Microbiol Biotechnol

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246

127

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The antimicrobial impact of biogenic-synthesized silver-based nanoparticles has been the focus of increasing interest. As the antimicrobial activity of nanoparticles is highly dependent on their size and surface, the complete and adequate characterization of the nanoparticle is important. This review discusses the characterization and antimicrobial activity of biogenic synthesized silver nanoparticles and silver chloride nanoparticles. By revising the literature, there is confusion in the characterization of these two silver-based nanoparticles, which consequently affects the conclusion regarding to their antimicrobial activities. This review critically analyzes recent publications on the synthesis of biogenic silver nanoparticles and silver chloride nanoparticles by attempting to correlate the characterization of the nanoparticles with their antimicrobial activity. It was difficult to correlate the size of biogenic nanoparticles with their antimicrobial activity, since different techniques are employed for the characterization. Biogenic synthesized silver-based nanoparticles are not completely characterized, particularly the nature of capped proteins covering the nanomaterials. Moreover, the antimicrobial activity of theses nanoparticles is assayed by using different protocols and strains, which difficult the comparison among the published papers. It is important to select some bacteria as standards, by following international foundations (Pharmaceutical Microbiology Manual) and use the minimal inhibitory concentration by broth microdilution assays from Clinical and Laboratory Standards Institute, which is the most common assay used in antibiotic ones. Therefore, we conclude that to have relevant results on antimicrobial effects of biogenic silver-based nanoparticles, it is necessary to have a complete and adequate characterization of these nanostructures, followed by standard methodology in microbiology protocols.

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Combination of fluconazole with silver nanoparticles produced byFusarium oxysporumimproves antifungal effect against planktonic cells and biofilm of drug-resistantCandida albicans

Cited by 68 publications

References 18 publications

Searching new antifungals: The use of in vitro and in vivo methods for evaluation of natural compounds

Searching new antifungals: The use of in vitro and in vivo methods for evaluation of natural compounds

Acinetobacter sp. mediated synthesis of AgNPs, its optimization, characterization and synergistic antifungal activity against C. albicans

Antimicrobial activity of biogenic silver nanoparticles, and silver chloride nanoparticles: an overview and comments

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