Combination of DNA walker and Pb2+-specific DNAzyme-based signal amplification with a signal-off electrochemical DNA sensor for Staphylococcus aureus detection

Advances in electrochemical-optical dual-mode biosensors for detection of environmental pathogens

“…DNA walkers could move autonomously along engineered DNA tracks and are broadly utilized in the biosensor and material assembly fields due to their continuous synthesis capability and significant mobility. 94 Single-signal enhancement by DNA walkers could directly generate electrochemical or fluorescent signals but has insufficient sensitivity for trace analysis. However, the signal effect and sensitivity can be improved by conjugating DNA walker amplification with HCR, RCA, and PCR.…”

Recent progress on DNAzyme-based biosensors for pathogen detection

“…137 They successfully detected Enterovirus EV71 and Another electrochemical method for detecting Staphylococcus aureus relied on DNA walker. 149 Researchers used two probes with RCD identifying Staphylococcus aureus and a magnetic bead with DNA substrate to achieve walking. One probe was modied with vancomycin, which had a high affinity for the peptidoglycan present on the cell wall of Gram-positive bacteria.…”

Rolling Circle Amplification/G‐Quadruplex‐Based Dual‐Signal Ratiometric Electrochemical Aptasensor for Ultrasensitive Detection of Pathogenic Bacteria

“…[36,37] Aptamers, as a potential biometric element, are integrated into the target with significant s1pecificity and high affinity to identify and detect target pathogens, and are often used in electrochemical biosensors to guarantee specificity. [38][39][40] Aptamers have significant advantages over antibodies in terms of thermal and chemical stability, cost effectiveness, and high reproducibility, as well as easy generation and modification. [41][42][43][44] Therefore, nucleic acid aptamerbased technologies continue to show potential for the diagnosis and treatment of various conditions, including infectious diseases.…”

“…For highly sensitive detection of specific bacteria, a set of ssDNA aptamers has been developed by the whole‐bacterial systematic evolution of ligands by exponential enrichment (SELEX) method [36,37] . Aptamers, as a potential biometric element, are integrated into the target with significant s1pecificity and high affinity to identify and detect target pathogens, and are often used in electrochemical biosensors to guarantee specificity [38–40] . Aptamers have significant advantages over antibodies in terms of thermal and chemical stability, cost effectiveness, and high reproducibility, as well as easy generation and modification [41–44] .…”