Combination of cell disruption technologies for lipid recovery from dry and wet biomass of Yarrowia lipolytica and using green solvents

Imatoukene, Nabila; Koubàa, Mohamed; Perdrix, Emile; Bénali, Mohammed; Vorobiev, Eugène

doi:10.1016/j.procbio.2019.11.011

Cited by 22 publications

(19 citation statements)

References 31 publications

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“…Table 1 summarizes the fatty acid profiles of oleaginous yeasts grown on different substrates and extracted by different mechanical and lipid extraction methods. Mechanical disruption methods are preferably used on wet biomass because of the high cost for dewatering or drying treatment [24]. However, extraction of dry biomass is more efficient compared to wet biomass and hence it is widely used for analysis purpose on small scale research [7].…”

Section: Pretreatment/cell Disruption Of Oleaginous Yeast To Extract mentioning

confidence: 99%

“…In general, the parameters that might affect the cell rupture performance via bead milling include agitator geometry, speed, slurry flow rate, ratio of bead to substrate, biomass loading, type and diameter of bead and processing time. The impacts of bead size on processing time for lipid extraction from Y. lipolytica JMY5289 in form of dry and wet biomass were studied by Imatoukene et al [24]. The experiment was conducted using a bead miller (MM400, Retsch GmbH, Germany) with stainless steel beads (sizes 2.9 and 4.9 mm) and glass beads (sizes 0.5, 1.0 and 2.0 mm) and processing times of 10, 20 and 30 s for three cycling times.…”

Section: Pretreatment/cell Disruption Of Oleaginous Yeast To Extract mentioning

confidence: 99%

“…To replace the conventional solvent, n-hexane, Imatoukene et al [24] investigated the use of several "green" solvents that are known as environmentally friendly, such as terpenes (p-cymene and d-limonene) and esters (isoamyl acetate, butyl acetate and ethyl acetate) for the extraction of lipids from Y. lipolytica JMY5289. Among the "green" solvents, the highest lipid yield was obtained by isoamyl acetate.…”

Section: "Green" Solvent For Lipid Recoverymentioning

confidence: 99%

“…The extraction with the combination of chloroform and methanol as developed by Folch and by Bligh and Dyer, these methods are the most cited methods for lipid extractions [22,23]. To further improve the extraction of lipids, several studies have also reported on the adaptation and modifications of both methods, whereas several authors have started to investigate the usage of "green" solvents that are considered environmentally friendly in comparison to the conventional solvents such as chloroform, methanol and hexane [24].…”

Section: Introductionmentioning

confidence: 99%

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Current Pretreatment/Cell Disruption and Extraction Methods Used to Improve Intracellular Lipid Recovery from Oleaginous Yeasts

et al. 2021

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The production of lipids from oleaginous yeasts involves several stages starting from cultivation and lipid accumulation, biomass harvesting and finally lipids extraction. However, the complex and relatively resistant cell wall of yeasts limits the full recovery of intracellular lipids and usually solvent extraction is not sufficient to effectively extract the lipid bodies. A pretreatment or cell disruption method is hence a prerequisite prior to solvent extraction. In general, there are no recovery methods that are equally efficient for different species of oleaginous yeasts. Each method adopts different mechanisms to disrupt cells and extract the lipids, thus a systematic evaluation is essential before choosing a particular method. In this review, mechanical (bead mill, ultrasonication, homogenization and microwave) and nonmechanical (enzyme, acid, base digestions and osmotic shock) methods that are currently used for the disruption or permeabilization of oleaginous yeasts are discussed based on their principle, application and feasibility, including their effects on the lipid yield. The attempts of using conventional and “green” solvents to selectively extract lipids are compared. Other emerging methods such as automated pressurized liquid extraction, supercritical fluid extraction and simultaneous in situ lipid recovery using capturing agents are also reviewed to facilitate the choice of more effective lipid recovery methods.

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Section: Pretreatment/cell Disruption Of Oleaginous Yeast To Extract mentioning

confidence: 99%

Section: Pretreatment/cell Disruption Of Oleaginous Yeast To Extract mentioning

confidence: 99%

Section: "Green" Solvent For Lipid Recoverymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Current Pretreatment/Cell Disruption and Extraction Methods Used to Improve Intracellular Lipid Recovery from Oleaginous Yeasts

et al. 2021

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“…60 However, a comparative study applying the green solvents 2-methyl tetrahydrofuran (2-MeTHF) and cyclopentyl methyl ether (CPME) revealed that the Bligh and Dyer method was more effective in extracting total lipids from the microalgae Chlorella pyrenoidosa for biodiesel production. 61 Although the potential of green solvents such as isoamyl acetate, 62 terpenes, ionic liquids, 58 2-methyltetrahydrofuran, 63 supercritical and subcritical fluids 64,65 for lipid extraction from yeast and microalgae has been evaluated, their applicability for plant lipid extraction is yet to be demonstrated. Future work will require the comparison of the traditional plant lipid extraction methods with the green solvent-based extraction methods to evaluate the applicability of green solvents in routine plant lipidomic analysis.…”

Section: Mol Omicsmentioning

confidence: 99%