Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts

Bakó, Csongor; Balázs, Viktória Lilla; Takács, Gyöngyi; Pallos, József Péter; Pál, Szilárd; Kocsis, Béla; Pethő, Dóra Rippelné; Horváth, Györgyi

doi:10.3390/molecules26216449

Cited by 6 publications

(2 citation statements)

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“…Supercritical carbon dioxide was used for the extraction of terpenes from powdered ginger rhizomes (Zingiber officinale) at 40 • C and 115 bar pressure [20] and carotenoids from annatto and fennel seeds (semipreparative scale) [50]. Solvent-assisted SFE was used to isolate the polar aromatic flavor compounds from cinnamon (details in Table 1) [35] and essential oil from clary sage (Salvia sclarea L.) with carbon dioxide containing 2% (v/v) ethanol at 40 • C and 186 bar pressure [51], and aloin and aloe-emodin from Aloe and Aloe-containing products [52]. Aloe-emodin (a trihydroxyanthracenedione) and aloin (a C-glycosylated derivative of aloe-emodin) are polar compounds only poorly extracted by supercritical fluid carbon dioxide.…”

Section: Paniculatementioning

confidence: 99%

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Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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High‐performance thin‐layer chromatography has favorable properties for high‐throughput separations with a high matrix tolerance. Sample preparation, however, is sometimes required to control specific matrix interferences and to enhance the detectability of target compounds. Trends in contemporary applications have shifted from absorbance and fluorescence detection to methods employing bioassays and mass spectrometry. Traditional methods (shake‐flask, heat at reflux, Soxhlet, and hydrodistillation) are being challenged by automated instrumental approaches (ultrasound‐assisted and microwave‐assisted solvent extraction, pressurized liquid extraction, and supercritical fluid extraction) and the quick, easy cheap, efficient, rugged, and safe extraction method for faster and streamlined sample processing. Liquid‐liquid extraction remains the most widely used approach for sample clean‐up with increasing competition from solid‐phase extraction. On‐layer sample, clean‐up by planar solid‐phase extraction is increasingly used for complex samples and in combination with heart‐cut multimodal systems. The automated spray‐on sample applicator, the elution head interface, biological detection of target and non‐target compounds, and straightforward mass spectrometric detection are highlighted as the main factors directing current interest toward faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants requiring high concentration factors.

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Section: Paniculatementioning

confidence: 99%

“…It contained visibly less colored material, and no further clean‐up was required prior to HPTLC analysis. It should be noted that several of the above studies demonstrated the preservation of biological activity of the extracts by HPTLC‐bioautography [20, 47, 48, 51]…”

Section: Solvent Extraction (Solids)mentioning

confidence: 99%