Combination of analyte protectants and sandwich injection to compensate for matrix effect of pesticides residue in GC–MS/MS

Soliman, Mostafa M.; Khorshid, Mona A.; Abo-Aly, M.M.

doi:10.1016/j.microc.2020.104852

Cited by 23 publications

(8 citation statements)

References 29 publications

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“…This relative signal suppression may be due to analytes' behavior in the GC inlet (liner and start of the column) that could be affected by the presence of other compounds from the matrix. 24 Matrix components may co-elute with the target analytes, causing ionization suppression in the mass spectrometer. On the other hand, 4 analytes presented positive matrix effects, namely: dicofol, cyfluthrin, p , p ′-DDT and chlorantraniliprole.…”

Section: Resultsmentioning

confidence: 99%

Holistic food safety evaluation of herbs: methods for the determination of organic and inorganic trace contaminants in Moringa stenopetala as a case study

Machado,

Gérez,

Bertón

et al. 2024

Anal. Methods

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Section: Resultsmentioning

confidence: 99%

Holistic food safety evaluation of herbs: methods for the determination of organic and inorganic trace contaminants in Moringa stenopetala as a case study

Machado,

Gérez,

Bertón

et al. 2024

Anal. Methods

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“…A reduction of more than three times was observed in the case of the compounds that showed a more significant matrix effect. Nevertheless, although the use of analyte protectants reduces the matrix effect and improves peak shape [135,136], these compounds must be dissolved in polar solvents such as water and MeCN. The injection of these solvents in GC systems has several drawbacks (i.e., limitations on injection volumes due to their high expansion coefficient, and poor focusing of chromatographic peaks due to their high polarity), which represents the main disadvantage of this strategy for reducing the matrix effect [69,126].…”

Section: Matrix Effectmentioning

confidence: 99%

Current Role of Mass Spectrometry in the Determination of Pesticide Residues in Food

Hernández‐Mesa

Moreno‐González

2022

Separations

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The extensive use of pesticides represents a risk to human health. Consequently, legal frameworks have been established to ensure food safety, including control programs for pesticide residues. In this context, the performance of analytical methods acquires special relevance. Such methods are expected to be able to determine the largest number of compounds at trace concentration levels in complex food matrices, which represents a great analytical challenge. Technical advances in mass spectrometry (MS) have led to the development of more efficient analytical methods for the determination of pesticides. This review provides an overview of current analytical strategies applied in pesticide analysis, with a special focus on MS methods. Current targeted MS methods allow the simultaneous determination of hundreds of pesticides, whereas non-targeted MS methods are now applicable to the identification of pesticide metabolites and transformation products. New trends in pesticide analysis are also presented, including approaches for the simultaneous determination of pesticide residues and other food contaminants (i.e., mega-methods), or the recent application of techniques such as ion mobility–mass spectrometry (IM–MS) for this purpose.

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“…Matrix effect must be considered in mass spectrometry, which means the change in the analytical signal is caused by anything in the sample matrix (33). Signal suppression or enhancement of the analyte due to the coelution of matrix components could influence the accuracy of the method (34)(35)(36). Matrix effect could be caused by compounds brought from complex matrices of analytical samples, solvents, reagents, and materials used in sample preparation or solvents, buffers, and additives contained in the mobile phase.…”

Section: Matrix Effectmentioning

confidence: 99%

Determination of Tropomyosin in Shrimp and Crab by Liquid Chromatography–Tandem Mass Spectrometry Based on Immunoaffinity Purification

Fan

et al. 2022

Front. Nutr.

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A UPLC-MS/MS method was developed for the detection of tropomyosin (TM) in shrimp and crab. After simple extraction, the samples were purified by immunoaffinity column and then digested by trypsin. The obtained sample was separated by Easy-nLC 1000-Q Exactive. The obtained spectrums were analyzed by Thermo Proteome Discoverer 1.4 software and then ANIQLVEK with high sensitivity was selected as the quantitative signature peptide. Isotope-labeled internal standard was used in the quantitative analysis. The method showed good linearity in the range of 5–5,000 μg/L with a limit of quantification (LOQ) of 0.1 mg/kg. The average recoveries were 77.22–95.66% with RSDs ≤ 9.97%, and the matrix effects were between 88.53 and 112.60%. This method could be used for rapid screening and quantitative analysis of TM in shrimp and crab. Thus, it could provide technical support for self-testing of TM by food manufacturers and promote further improvement of allergen labeling in China.

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Combination of analyte protectants and sandwich injection to compensate for matrix effect of pesticides residue in GC–MS/MS

Cited by 23 publications

References 29 publications

Holistic food safety evaluation of herbs: methods for the determination of organic and inorganic trace contaminants in Moringa stenopetala as a case study

Holistic food safety evaluation of herbs: methods for the determination of organic and inorganic trace contaminants in Moringa stenopetala as a case study

Current Role of Mass Spectrometry in the Determination of Pesticide Residues in Food

Determination of Tropomyosin in Shrimp and Crab by Liquid Chromatography–Tandem Mass Spectrometry Based on Immunoaffinity Purification

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