Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin – A comparative in vitro study

Kyyak, Solomiya; Blatt, Sebastian; Pabst, Andreas; Thiem, Daniel G. E.; Al‐Nawas, Bilal; Kämmerer, Peer W.

doi:10.1177/0885328220914407

Cited by 42 publications

(55 citation statements)

References 59 publications

(87 reference statements)

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“…Although biochemical signals are released from i-PRF, their effects on cells are limited and, surprisingly, not concentration-dependant [ 21 ]. Several studies found a wide range of biocompatibility using 20‒100% conditioned media from i-PRF on human fibroblasts [ 17 ] and osteoblasts [ 8 , 21 , 22 ]. Optimal cell viability also varied over 1‒5 d [ 17 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

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Red and Yellow Injectable Platelet-Rich Fibrin Demonstrated Differential Effects on Periodontal Ligament Stem Cell Proliferation, Migration, and Osteogenic Differentiation

Thanasrisuebwong

Kiattavorncharoen

Surarit

et al. 2020

IJMS

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The biological benefits of using two fractions derived from injectable platelet-rich fibrin (i-PRF) in bone regeneration remain unclear. Thus, the current study examined two fractionation protocols producing yellow i-PRF and red i-PRF on periodontal ligament stem cells (PDLSCs). The i-PRF samples from five donors were harvested from two different levels, with and without a buffy coat layer, to obtain red and yellow i-PRF, respectively. The PDLSCs were isolated and characterized before their experimental use. The culture medium in each assay was loaded with 20% of the conditioned medium containing the factors released from the red and yellow i-PRF. Cell proliferation and cell migration were determined with an MTT and trans-well assay, respectively. Osteogenic differentiation was investigated using alkaline phosphatase and Alizarin red staining. The efficiency of both i-PRFs was statistically compared. We found that the factors released from the red i-PRF had a greater effect on cell proliferation and cell migration. Moreover, the factors released from the yellow i-PRF stimulated PDLSC osteogenic differentiation earlier compared with the red i-PRF. These data suggest that the red i-PRF might be suitable for using in bone regeneration because it induced the mobilization and growth of bone regenerative cells without inducing premature mineralization.

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Section: Discussionmentioning

confidence: 99%

“…Clinicians use i-PRF in combination with particulate bone grafts to promote the biological and physical properties of the materials [ 8 ]. The combination has shown benefits, such as improved angiogenesis and handling ability by combining the small bone granules into a bulk material to use in bone grafting surgery [ 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Red and Yellow Injectable Platelet-Rich Fibrin Demonstrated Differential Effects on Periodontal Ligament Stem Cell Proliferation, Migration, and Osteogenic Differentiation

Thanasrisuebwong

Kiattavorncharoen

Surarit

et al. 2020

IJMS

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“…The porcine-derived type I and III collagen membrane has a bilayer structure, consisting of a compact outer layer and a porous inner layer of collagen fiber bundles [10]. Both materials, alone and in combination by means of GBR-procedures, are frequently used in preclinical as well as clinical regenerative maxillofacial surgery [3,18,[30][31][32].…”

Section: Study Materialsmentioning

confidence: 99%

A collagen membrane influences bone turnover marker in vivo after bone augmentation with xenogenic bone

et al. 2020

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Background The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membrane in vivo. Methods Eight weeks after formation of a lateral full-thickness perforating bone defect in the mandible of 40 rabbits, bovine bone substitute with (“+”;n = 20) and without (“-”;n = 20) collagen membrane was applied. Blood and bone was collected 24, 72 h, 7, 14 and 21 days after surgery. Total acid phosphatase, bone acid phosphatase, total alkaline phosphatase and bone alkaline phosphatase activities were compared between groups. Formation of new bone was quantified histologically for all time points. Results Twenty-four hours after surgery, bone alkaline phosphatase was significantly elevated in “+” group when compared to “-” (p=0.012). After 72 hours, all bone turnover markers except for total acid phosphatase (p=0.078) where significantly elevated in “+” (all p < 0.05). Fourteen days after surgery, the significant highest values for all bone turnover markers were detected in “-” (all p < 0.05). A significant difference in favor of group “-” could also be detected after 3 weeks in terms of both acid phosphatases (p < 0.05). In histology, no significant differences could be detected. Conclusion Bone regeneration with bovine bone substitute material and collagen membrane shows a significantly earlier bone remodeling activity but does not seem to influence formation of new bone in histological samples.

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“…Tissue engineering in the maxillofacial region aims for regeneration of soft and hard tissue deficiencies in order to sustain oral-related health as well as to improve masticatory function. As autograft harvesting results in increased local morbidity, allogeneic, alloplastic, and xenogeneic grafts are frequently used, even though autogenous tissue remains the biological gold standard [ 1 , 2 , 3 ]. Most studies in biomaterial research mainly focus on the mechanical and functional properties of the material [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Modulation of Angiogenesis and Immune Response on a Collagen Matrix via Extracorporeal Shockwaves

Heimes

Wiesmann

Eckrich³

et al. 2020

IJMS

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The effective management of tissue integration and immunological responses to transplants decisively co-determines the success of soft and hard tissue reconstruction. The aim of this in vivo study was to evaluate the eligibility of extracorporeal shock wave therapy (ESWT) with respect to its ability to modulate angiogenesis and immune response to a collagen matrix (CM) for tissue engineering in the chorioallantoic membrane (CAM) assay, which is performed with fertilized chicken eggs. CM were placed on the CAM on embryonic development day (EDD) 7; at EDD-10, ESWT was conducted at 0.12 mJ/mm2 with 500 impulses each. One and four days later, angiogenesis represented by vascularized area, vessel density, and vessel junctions as well as HIF-1α and VEGF gene expression were evaluated. Furthermore, immune response (iNOS2, MMP-9, and MMP-13 via qPCR) was assessed and compared between ESWT- and non-ESWT-groups. At EDD-14, the vascularized area (+115% vs. +26%) and the increase in vessel junctions (+751% vs. +363%) were significantly higher in the ESWT-group. ESWT significantly increased MMP-9 gene expression at EDD-11 and significantly decreased MMP-13 gene expression at EDD-14 as compared to the controls. Using the CAM assay, an enhanced angiogenesis and neovascularization in CM after ESWT were observed. Furthermore, ESWT could reduce the inflammatory activity after a latency of four days.

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Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin – A comparative in vitro study

Cited by 42 publications

References 59 publications

Red and Yellow Injectable Platelet-Rich Fibrin Demonstrated Differential Effects on Periodontal Ligament Stem Cell Proliferation, Migration, and Osteogenic Differentiation

Red and Yellow Injectable Platelet-Rich Fibrin Demonstrated Differential Effects on Periodontal Ligament Stem Cell Proliferation, Migration, and Osteogenic Differentiation

A collagen membrane influences bone turnover marker in vivo after bone augmentation with xenogenic bone

In Vivo Modulation of Angiogenesis and Immune Response on a Collagen Matrix via Extracorporeal Shockwaves

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