Combination of Adsorption and Cellulose Derivative Membrane Coating for Efficient Immobilization of Laccase

Wu, Rong; Liu, Feixiang; Dong, Qihui; Qiu, Yibin; Sun, Yiyi; Su, Erzheng

doi:10.1007/s12010-020-03446-z

Cited by 9 publications

(4 citation statements)

References 40 publications

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“…However, in this study, the decolorization rate by B.P-Lacc was very low in the absence of mediators. Acid black ATT and Acid red A35 were difficult to be decolorized by the recombinant laccase, and less than 60% decolorization rate was observed at any laccase/mediator systems during 4 h incubation, which was lower than the immobilized laccases applied to the indigo decolorization reported by Wu et al By adding 1.0 mM ABTS, more than 90% of BRRB was decolorized by the enzyme, whether at pH 4.0 or pH 7.9.…”

Section: Results and Discussionmentioning

confidence: 71%

Cloning, Overexpression, and Characterization of a Thermostable, Organic Solvent-Tolerant Laccase from Bacillus pumilus ARA and Its Application to Dye Decolorization

et al. 2021

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A thermostable and organic solvent-tolerant bacterial laccase from Bacillus pumilus ARA has been expressed heterologously and characterized, which shows potential decolorization capacity to various types of industrial synthetic dyes. The optimal temperature and pH were 85 °C and 3.5, respectively, while the purified recombinant laccase B.P.Lacc was stable under 55–75 °C and pH 5.0–8.0 conditions. The apparent kinetic parameters K m and V max of B.P.Lacc for ABTS as the substrate were 0.33 mM and 32.4 U/mg, respectively. Ethanol (1%, v/v) and methanol (2%, v/v) could stimulate the enzyme activity. The recombinant laccase retained over 95% of its initial activity in 10% (v/v) methanol. The optimal expression conditions for the laccase production of B.P.Lacc in LB medium were obtained: induction temperature of 25 °C, 0.4 mM Cu 2+ , and 1.0 mM IPTG added into the culture. After 5 h, the final laccase production was 1283 U/mL. Moreover, the laccase activity increased to 4822 U/mL after follow-up 2 h stationary cultivation, with about a 3.76-fold increase. The purified B.P.Lacc was able to efficiently decolorize synthetic dyes combined with mediators. Adding 1.0 mM ABTS, more than 90% of BRRB was decolorized by the enzyme, whether at pH 4.0 or pH 7.9. The outstanding enzymatic properties suggested that B.P.Lacc may be suitable for a wide application in future biodegradation fields.

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Section: Results and Discussionmentioning

confidence: 71%

Cloning, Overexpression, and Characterization of a Thermostable, Organic Solvent-Tolerant Laccase from Bacillus pumilus ARA and Its Application to Dye Decolorization

et al. 2021

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“…Cellulose is the most abundant natural polymer and is nontoxic, biodegradable, hydrophilic, and biocompatible making it an excellent support for enzyme immobilization [ 313 ]. In addition, its derivatives such as cellulose acetate, cellulose nitrate, and carboxymethyl cellulose also offer excellent biocompatibility [ 168 , 532 ]. Cellulose usually varies in morphology, size, crystallinity, and surface functional groups depending on the source of agricultural waste used during synthesis [ 247 ].…”

Section: Supports Used For Laccase Immobilizationmentioning

confidence: 99%

Applications and immobilization strategies of the copper-centred laccase enzyme; a review

Kyomuhimbo¹,

Brink²

2023

Heliyon

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“…The search for sustainable, reusable, environmentally friendly, and economically favorable potential bioadsorbents has directed attention toward abundantly available plant-based biomasses as well as agricultural and industrial , wastes. Enzymes immobilized on cellulose-based materials have also been used for degrading pollutants for water purification. , Heavy metal adsorption on fruit peels, , plant leaves, rice husk, peanut waste, corn waste, egg shells, tea waste, coconut coir pith, and sugar cane bagasse has been widely investigated in the recent past. The combination of low cost, multifunctionality, biodegradability, and renewability of plant-based adsorbents makes them an ideal choice for heavy metal ion removal from wastewater.…”

Section: Introductionmentioning

confidence: 99%

“…Enzymes immobilized on cellulose-based materials have also been used for degrading pollutants for water purification. 53,54 Heavy metal adsorption on fruit peels, 55,56 plant leaves, 57 rice husk, 58 peanut waste, 59 corn waste, 60 egg shells, 61 tea waste, 62 coconut coir pith, 63 and sugar cane bagasse 64 has been widely investigated in the recent past. The combination of low cost, multifunctionality, biodegradability, and renewability of plant-based adsorbents makes them an ideal choice for heavy metal ion removal from wastewater.…”

Section: Introductionmentioning

confidence: 99%

Critical Review of Bioadsorption on Modified Cellulose and Removal of Divalent Heavy Metals (Cd, Pb, and Cu)

Kaur

Sengupta

Mukhopadhyay³

2022

Ind. Eng. Chem. Res.

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Heavy metal contamination in soil and water has become a serious threat to all forms of life. Finding a sustainable remediation method is the prime concern of researchers worldwide. In this quest, cellulose has emerged as a biocompatible, biodegradable, renewable, tunable, and cost-effective bioadsorbent for heavy metal removal from polluted water. Cellulose is rich in hydroxyl groups which can be chemically modified to enhance the reactivity, binding sites, and mechanical strength. Cellulose in nano size, i.e., nanofibers and nanocrystals, offer myriad opportunities to improve the aspect ratio, surface area, pore size, and hydrophilicity for effective adsorption. In this review, chemical and physical modifications in cellulose (functional group transformations, nanocellulose, nanocomposites, hydrogels, aerogels, beads, and membranes) with respect to adsorption of divalent heavy metal ions, in particular, Pb 2+ , Cu 2+ , and Cd 2+ , have been extensively discussed. This review also examines the effect of pH, concentration, temperature, and contact time on the adsorption process. Different mechanisms for heavy metal removal such as ion exchange, electrostatic interactions, complexation, chelation, precipitation, and reduction have been discussed. Theoretical insights into the mechanisms of adsorbent−adsorbate binding are critically examined in the context of modified cellulose. The authors, in the concluding section, summarize potential research directions toward developing sustainable biosorbents.

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Combination of Adsorption and Cellulose Derivative Membrane Coating for Efficient Immobilization of Laccase

Cited by 9 publications

References 40 publications

Cloning, Overexpression, and Characterization of a Thermostable, Organic Solvent-Tolerant Laccase from Bacillus pumilus ARA and Its Application to Dye Decolorization

Cloning, Overexpression, and Characterization of a Thermostable, Organic Solvent-Tolerant Laccase from Bacillus pumilus ARA and Its Application to Dye Decolorization

Applications and immobilization strategies of the copper-centred laccase enzyme; a review

Critical Review of Bioadsorption on Modified Cellulose and Removal of Divalent Heavy Metals (Cd, Pb, and Cu)

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