Combination of a nanocarrier delivery system with genetic manipulation further improves pesticide efficiency: a case study with chlorfenapyr

Yan, Shuo; Yin, Hujun; Li, Na; Chen, Yao; Ji, Chendong; Jiang, Qinhong; Du, Juan; Yin, Meizhen; Shen, Jie; Zhang, Junzheng

doi:10.1039/d2en00126h

Cited by 11 publications

(10 citation statements)

References 56 publications

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“…Nanocarriers can greatly enhance the efficiency of delivery of pesticides, improve the bioactivity of synthetic pesticides and decrease the amount of pesticide residues ( Jiang et al, 2022 ; Yan et al, 2022 ), thus, showing great potential for green pest management ( Dong et al, 2022 ). Shen jie’s team conducted research on the mechanism and delivery process of RNAi mediated by nanoparticles and proposed that the efficient protection and delivery of dsRNA by nanocarriers was the key factor that enhanced the efficiency of RNAi ( Ma et al, 2022 ).…”

Section: Nanocarriers -- a Viable Solution For Dsrna Stabilizationmentioning

confidence: 99%

Advances in nanocarriers to improve the stability of dsRNA in the environment

Yang

Wang

Gao

et al. 2022

Front. Bioeng. Biotechnol.

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RNAi technology, known as a revolutionary technology in the history of pesticides, has been identified as a very promising novel approach for crop protection, which is of great significance for achieving the sustainable agricultural development of the United Nations Food and Agriculture Organization. Although many studies have shown that RNA biopesticides have strong application prospects, its stability seriously restricts the commercial use. As the core component of RNAi, double-stranded RNA (dsRNA) is unstable in its natural form. Therefore, how to ensure the stability of dsRNA is one of the most significant challenges in realizing the commercial use of RNA biopesticides. Nanomaterials such as cationic polymers and lipofectamine can improve the stability of dsRNA in the environment, which has been proved. This paper reviews the recent research progress of nanomaterials that can be used to improve the environmental stability of dsRNA, and discusses the advantages and limitations of different nanomaterials combined with dsRNA, which provides reference for the selection of dsRNA nanoformulations.

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Section: Nanocarriers -- a Viable Solution For Dsrna Stabilizationmentioning

confidence: 99%

Advances in nanocarriers to improve the stability of dsRNA in the environment

Yang

Wang

Gao

et al. 2022

Front. Bioeng. Biotechnol.

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“…However, a number of obstacles, such as the vulnerability of dsRNA in the ambient environment and the physiological milieu (particularly in the digestive tract, e.g., the midgut) and difficulties in the uptake of dsRNA, have resulted in highly variable responses using dsRNA for the treatment of insect pests . To overcome these obstacles, various delivery vehicles, including chitosan and derivatives, − liposomes, ,− dendrimers, − linear polymers, − branched amphiphilic peptide capsules, and inorganic nanoparticles, have been reported for the transportation of dsRNA into intracellular targets. However, these delivery vehicles lack a rational spatial architectural arrangement at the nanoscale level, which is conjectured to be difficult in affording sufficient functionalities in circumventing the pre-defined barriers in the journey of RNAi delivery, consequently leading to unsatisfactory insecticidal outcomes.…”

Section: Introductionmentioning

confidence: 99%

Synthetic Nanoscale RNAi Constructs as Pesticides for the Control of Locust Migratoria

Cui

et al. 2022

J. Agric. Food Chem.

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The low efficiency of RNA interference (RNAi) in insects via the oral administration of double-stranded RNA (dsRNA) is a considerable obstacle preventing its application in insect pest control. The instability of dsRNA and insufficient dsRNA uptake are known to limit the RNAi efficiency. To overcome these limitations, the block copolymer poly(ethylene glycol)polylysine(thiol) [PEG-PLys(SH)] was designed in this study to form well-defined, core−shell nanoparticles to protect dsRNA from premature degradation and to facilitate its movement through various physiological barriers. The developed material had excellent structural stability and dsRNA-protecting capacity, thereby enabling the prolonged survival of dsRNA in the digestive tract for endocytosis into the midgut cells of the migratory locust, Locusta migratoria. After encapsulation of a dsLmCHS2 payload (a midgut gene), a 60% down-regulation of LmCHS2, accompanied with observations of amorphous and discontinuous linings of the peritrophic matrix and abnormal phenotypes, was observed. In addition, the elaborated nanoscale dsRNA condensates appeared to readily extravasate through the narrow fenestrations in the linings of midgut epithelial cells into the hemolymph and be distributed throughout the body. After encapsulation of a dsLmCHS1 payload (a cuticle gene), a distinctive lethal phenotype with molting failure was observed as a result of a 50% down-regulation in LmCHS1. The persistent leaf adherence of these dsRNA constructs was also capable of resisting continuous rinsing. Therefore, these dsRNA constructs represent a robust type of RNAi pesticide, which has potential as a versatile pesticide against a variety of molecular targets for the control of destructive insects and insects resistant to conventional pesticides.

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“…Our studying group has designed and constructed a facile-synthesized star polycation (SPc) for dsRNA and pesticide delivery (Li et al 2019;Yan et al 2019Yan et al , 2022Jiang et al 2022;Ma et al 2022b). A SPcmediated transdermal dsRNA delivery system is established and has been applied to control Grapholita molesta via inhibiting feeding behavior (Yan et al 2020;Wei et al 2021).…”

Section: Introductionmentioning

confidence: 99%

RNA interference cannot be operated in lepidopteran insect? A nanocarrier breaks bottlenecks at all developmental stages of Spodoptera frugiperda

Chao

Zhang

et al. 2022

Preprint

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RNA interference (RNAi) has become an essential technique in functional analysis of insect genes and pest management. However, insensitivity to RNAi is the main obstacle that limits the identification of functional genes in lepidopteran insects. Hereby we explored an efficient nanocarrier (star polycation, SPc)-mediated dsRNA delivery system to achieve efficient RNAi at all developmental stages of lepidopteran insect Spodoptera frugiperda. Double-stranded RNA (dsRNA) was synthesized targeting a consistently expressed gene V-type proton ATPase subunit d (ATP-d) as well as enhanced green fluorescent protein (eGFP) for various operation tests. At the egg and early larval stages, SPc-loaded dsRNA could penetrate the physical obstacles of eggshell and larval body wall. Thus, soaking and topical applications of SPc-loaded dsRNA were suitable for efficient RNAi at egg and 1st-2nd instar larval stages, respectively. The SPc could protect dsRNA from degradation by midgut fluid of mid-late instar larvae. Thereby oral feeding of SPc-loaded dsRNA silenced the target gene at mid-late larval stages more efficiently compared to dsRNA alone. Finally, for pupal and adult stages, injection of SPc-loaded dsRNA was enough to induce high RNAi effect. A key developmental gene, Chitin deacetylase 1 (CDA1), was taken as an example to test the pest control efficiency of SPc-based RNAi system. The constructed system could remarkably induce the knockdown of CDA1 and lead to growth retardation and mortality. Overall, the present study constructed an efficient nanocarrier-based RNAi system for whole developmental stages of lepidopteran insects, which would provide a powerful tool for identifying functional genes in lepidopteran insects.

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Combination of a nanocarrier delivery system with genetic manipulation further improves pesticide efficiency: a case study with chlorfenapyr

Abstract: Excessive usage of pesticides poses great risks towards global environment. Reducing pesticides usage by improving the insecticidal efficiency is essential for environment protection. Delivery by nanocarriers has shown great potential...

Cited by 11 publications

References 56 publications

Advances in nanocarriers to improve the stability of dsRNA in the environment

Advances in nanocarriers to improve the stability of dsRNA in the environment

Synthetic Nanoscale RNAi Constructs as Pesticides for the Control of Locust Migratoria

RNA interference cannot be operated in lepidopteran insect? A nanocarrier breaks bottlenecks at all developmental stages of Spodoptera frugiperda

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