Combination of 5-Florouracil and polyphenol EGCG exerts suppressive effects on oral cancer cells exposed to radiation

Pons-Fuster, Eduardo; García, Francisco Gómez; Jornet, Pía López

doi:10.1016/j.archoralbio.2019.02.018

Cited by 27 publications

(17 citation statements)

References 28 publications

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“…The findings were in line with the results in head and neck cancer that EGCG exhibited synergistic apoptosis is induction effects in combination with chemotherapy erlotinib (Haque et al, 2015). EGCG was also demonstrated to be effective in reducing cell viability and migration in oral cancer when combined with 5fluorouracil (Pons-Fuster López et al, 2019). In addition, the combination of DOX and EGCG showed greater level inhibition of cell migration on T24 and SW780 cells, when compared with individual treatment of DOX or EGCG, as assessed by scratch wound healing and transwell migration assays (Figure 3).…”

Section: Discussionsupporting

confidence: 89%

EGCG Enhanced the Anti-tumor Effect of Doxorubicine in Bladder Cancer via NF-κB/MDM2/p53 Pathway

Luo

Zhu

Zhao

et al. 2020

Front. Cell Dev. Biol.

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Doxorubicin (DOX), the first-line chemotherapy for bladder cancer, usually induces side effects. We previously demonstrated that green tea polyphenol EGCG had potent anti-tumor effect in bladder cancer via down regulation of NF-κB. This study aimed to investigate the additive/synergistic effect EGCG and DOX against bladder cancer. Our results demonstrated that the combined use of DOX and EGCG inhibited T24 and SW780 cell proliferation. EGCG enhanced the apoptosis induction effect of DOX in both SW780 and T24 cells and resulted in significant differences. Besides, EGCG promoted the inhibitory effect of DOX against bladder cancer cell migration. In addition, the in vivo results demonstrated that DOX in combination with EGCG showed the most potent anti-tumor effects among DOX, EGCG and DOX+EGCG treatment groups. Further mechanistic studies determined that the combination of DOX and EGCG inhibited phosphorylated NF-κB and MDM2 expression, and up-regulated p53 expression in tumor, as assessed by western blot and immunohistochemistry. Western blot in SW780 cells also confirmed that the combined use of EGCG and DOX caused significant increase in p53, p21, and cleaved-PARP expression, and induced significant inhibition in phosphorylated NF-κB and MDM2. When NF-κB was inhibited, the expression of p53 and p-MDM2 were changed, and the combination of DOX and EGCG showed no obvious effect in transwell migration and cell viability. In conclusion, the novel application of chemotherapy DOX and EGCG demonstrated potent anti-tumor, anti-migration and anti-proliferation effects against bladder cancer. EGCG enhanced the anti-tumor effect of DOX in bladder cancer via NF-κB/MDM2/p53 pathway, suggesting the potential clinical application against bladder cancer patients.

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Section: Discussionsupporting

confidence: 89%

EGCG Enhanced the Anti-tumor Effect of Doxorubicine in Bladder Cancer via NF-κB/MDM2/p53 Pathway

Luo

Zhu

Zhao

et al. 2020

Front. Cell Dev. Biol.

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“…High doses of 5-FU are used for long-duration cancer treatments, leading to the development of drug resistance and the incidence of systemic adverse effects [ 53 , 54 ]. Combination therapy using reduced doses of 5-FU and compounds of natural origin exhibiting anticancer activity has been shown to enhance the suppression of cell viability compared to monotherapy with high doses of 5-FU [ 55 ]. Combination with plant extracts has also been shown to abolish resistance to 5-FU [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Calotropis gigantea stem bark extract induced apoptosis related to ROS and ATP production in colon cancer cells

et al. 2021

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Conventional chemotherapeutic agents for colorectal cancer (CRC) cause systemic side effects and eventually become less efficacious owing to the development of drug resistance in cancer cells. Therefore, new therapeutic regimens have focused on the use of natural products. The anticancer activity of several parts of Calotropis gigantea has been reported; however, the effects of its stem bark extract on inhibition of cancer cell proliferation have not yet been examined. In this study, the anticancer activity of C. gigantea stem bark extract, both alone and in combination with 5-fluorouracil (5-FU), was evaluated. A crude ethanolic extract was prepared from dry, powdered C. gigantea barks using 95% ethanol. This was then partitioned to obtain dichloromethane (CGDCM), ethyl acetate, and water fractions. Quantitative analysis of the constituent secondary metabolites and calotropin was performed. These fractions exhibited cytotoxicity in HCT116 and HT-29 cells, with CGDCM showing the highest potency in both the cell lines. A combination of CGDCM and 5-FU significantly enhanced the cytotoxic effect. Moreover, the resistance of normal fibroblast, HFF-1, cells to this combination demonstrated its safety in normal cells. The combination significantly enhanced apoptosis through the mitochondria-dependent pathway. Additionally, the combination reduced adenosine triphosphate production and increased the production of reactive oxygen species, demonstrating the mechanisms involved in the induction of apoptosis. Our results suggest that CGDCM is a promising anti-cancer agent and may enhance apoptosis induction by 5-FU in the treatment of CRC, while minimizing toxicity toward healthy cells.

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“…Moreover, J. communis has been reported to inhibit breast cancer proliferation [ 21 ] and neuroblastoma [ 22 ]; however, the inhibitory potential of J. communis extract (JCo) on oral cancer was still not clear. Furthermore, 5-Fluorouracil (5-FU) is a chemotherapeutic drug which is a pyrimidine analog, and its metabolite can incorporate into RNA and DNA, or inhibit thymidylate synthetase [ 23 ]. 5-FU has been widely used alone or in combination with other anticancer agents and/or radiotherapy for treating various types of cancer, such as head and neck, and colorectal cancer.…”

Section: Introductionmentioning

confidence: 99%

Suppression of oral cancer by induction of cell cycle arrest and apoptosis using Juniperus communis extract

et al. 2020

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The oral cancer incidence rate is slowly increasing and is now the fifth leading cause of cancer-related death due to its high metastasis and recurrence rate. Juniperus communis is used as a traditional Chinese medicine and has been proven to have anti-cancer activity against neuroblastomas. In this study, we further investigated the anti-cancer mechanisms of J. communis extract (JCo) on oral cancer and evaluated the synergistic effects of JCo combined with 5-fluorouracil (5-FU). We found that JCo inhibited oral cancer cell growth, and that JCo might be less cytotoxic to normal cells than to cancer cells. After JCo treatment, cell cycle arrest was observed at the G0/G1 phase through modulation of p53/p21 and Rb signaling. JCo also caused an increase in the sub-G1 phase and cell apoptosis via the intrinsic and extrinsic apoptosis pathways. JCo combined with 5-FU presented a synergistic effect to reduce cell viability. In conclusion, JCo inhibited oral cancer cell growth by inducing cell cycle arrest and activating cell apoptosis, and JCo significantly synergized with 5-FU. JCo might have the potential to be an adjuvant and a new therapeutic drug for oral cancer treatment.

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Combination of 5-Florouracil and polyphenol EGCG exerts suppressive effects on oral cancer cells exposed to radiation

Cited by 27 publications

References 28 publications

EGCG Enhanced the Anti-tumor Effect of Doxorubicine in Bladder Cancer via NF-κB/MDM2/p53 Pathway

EGCG Enhanced the Anti-tumor Effect of Doxorubicine in Bladder Cancer via NF-κB/MDM2/p53 Pathway

Calotropis gigantea stem bark extract induced apoptosis related to ROS and ATP production in colon cancer cells

Suppression of oral cancer by induction of cell cycle arrest and apoptosis using Juniperus communis extract

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