Comamonas testosteroni bacteremia: A rare unusual pathogen detected in a burned patient: Case report and literature review

Sammoni, Ayham; Abdalah, Ali; Al-Aissami, Maen

doi:10.1016/j.amsu.2022.103371

Cited by 6 publications

(1 citation statement)

References 26 publications

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“…This organism frequently grows in a wide range of habitats, such as wastewater, wetlands, soil, and hospital environments 2 , and has been reported as one of the major members of microbial communities in wastewater bioaugmentation and bioremediation 3 . In spite of its uncommon pathogenesis, some Comamonas species have been increasingly reported to be closely associated with invasive infections in humans, such as bacteremia 4 , 5 , urinary tract infection 6 , intra-abdominal infection 7 , and meningitis 8 .…”

Section: Introductionmentioning

confidence: 99%

A new class A beta-lactamase gene blaCAE-1 coexists with blaAFM-1 in a novel untypable plasmid in Comamonas aquatica

Liu

Fang

Wang

et al. 2023

Sci Rep

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Antimicrobial resistance, especially carbapenem resistance, poses a serious threat to global public health. Here, a carbapenem-resistant Comamonasaquatica isolate SCLZS63 was recovered from hospital sewage. Whole-genome sequencing showed that SCLZS63 has a 4,048,791-bp circular chromosome and three plasmids. The carbapenemase gene blaAFM-1 is located on the 143,067-bp untypable plasmid p1_SCLZS63, which is a novel type of plasmid with two multidrug-resistant (MDR) regions. Notably, a novel class A serine β-lactamase gene, blaCAE-1, coexists with blaAFM-1 in the mosaic MDR2 region. Cloning assay showed that CAE-1 confers resistance to ampicillin, piperacillin, cefazolin, cefuroxime, and ceftriaxone, and elevates the MIC of ampicillin-sulbactam two-fold in Escherichia coli DH5α, suggesting that CAE-1 functions as a broad-spectrum β-lactamase. Amino acid sequences analysis suggested that blaCAE-1 may originate from Comamonadaceae. The blaAFM-1 in p1_SCLZS63 is located in a conserved structure of ISCR29-ΔgroL-blaAFM-1-ble-ΔtrpF-ΔISCR27-msrB-msrA-yfcG-corA. Comprehensive analysis of the blaAFM-bearing sequences revealed important roles of ISCR29 and ΔISCR27 in the mobilization and truncation of the core module of blaAFM alleles, respectively. The diverse passenger contents of class 1 integrons flanking the blaAFM core module make the complexity of genetic contexts for blaAFM. In conclusion, this study reveals that Comamonas may act as an important reservoir for antibiotics-resistance genes and plasmids in the environment. Continuous monitoring for the environmental emergence of antimicrobial-resistant bacteria is needed to control the spread of antimicrobial resistance.

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