Comamonas humi sp. nov., isolated from soil

Abstract

A bacterial strain, designated GAU11 T , was isolated from soil in Japan. Cells of the strain were Gram-stain-negative, aerobic, non-motile rods. The 16S rRNA gene sequence of strain GAU11 T showed high similarity to those of Comamonas zonglianii BF-3 T (98.8 %), Pseudacidovorax intermedius CC21 T (96.4 %), Acidovorax caeni R-24608 T (96.2 %), Alicycliphilus denitrificans K601 T (96.2 %), Pseudorhodoferax soli TBEA3 T (95.9 %) and Comamonas terrigena LMG 1253 T (95.9 %). Strain GAU11 T contained ubiquinone 8 as the sole ubiquinone and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. Its major cellular fatty acids were C 16 : 0 , C 18 : 1 v7c and summed feature 3 (C 16 : 1 v7c and/or iso-C 15 : 0 2-OH). The DNA G+C content of strain GAU11 T was 68.2 mol%. The DNA-DNA relatedness between strain GAU11 T and C. zonglianii DSM 22523 T was 52 or 68 % (reciprocal value). Phenotypic characterization indicated that strain GAU11 T represents a member of the genus Comamonas, but at the same time distinguished it from C. zonglianii DSM 22523 T . From polyphasic characterization, this strain should be classified as representing a novel species of the genus Comamonas, for which the name Comamonas humi sp. nov. (type strain GAU11 T 5JCM

Flavobacterium aquicola sp. nov., isolated from river water

“…The Gram reaction of cells was performed by the traditional staining method and nonstaining (KOH) method (Buck, 1982). Catalase and oxidase activities were tested according to previously described methods (Hatayama, 2014). Hydrolysis of CM-cellulose (Wako Pure Chemical Industries) (0.5 %, w/v), casein (Wako Pure Chemical Industries) (1 %, w/v), chitin (Wako Pure Chemical Industries) (0.5 %, w/v), pectin (Wako Pure Chemical Industries) (0.5 %, w/v), starch (Nacalai Tesque) (0.2 %, w/v), L-tyrosine (Nacalai Tesque) (0.5 %, w/v) and egg yolk (5 %, v/v) was tested using R2A agar as the basal medium.…”

Saezia sanguinis gen. nov., sp. nov., a Betaproteobacteria member of order Burkholderiales, isolated from human blood

“…com/). The order comprises Gram-negative microorganisms, mostly isolated from soil, water and vegetation, but which can infect plants and animals [1][2][3][4]. Species such as Stenotrophomonas maltophilia, Pandoraea apista, Ralstonia picketii, Achromobacter xylosoxidans, and members of the Burkholderia cepacia complex (Bcc) can behave as opportunistic human pathogens that mainly affect immunocompromised people [5].…”

“…Differential phenotypic, genomic and genetic characteristics of strain CNM695-12 and type strains of related species belonging to family Comamonadaceae Strains: 1, CNM695-12; 2, Comamonas terrigena Hugh 247 T (data from[2,58]; 3, Ramlibacter tataouinensis TTB310 T (data from[54]. +, Positive; −, negative; w, weakly positive; nd, data no available.…”

Comamonas phosphati sp. nov., isolated from a phosphate mine

“…The genus comprises Gram-stain-negative, chemo-organotrophic, rods or spirilla with hexadecanoic acid (C 16 : 0 ), hexadecenoic acid (C 16 : 1 ) and octadecenoic acid (C 18 : 1 ) as the major fatty acids (Chipirom et al, 2012;Chou et al, 2007;Willems & Gillis, 2005;Zhang et al, 2013). Except Comamonas acidovorans, which was renamed as Delftia acidovorans and excluded from the genus Comamonas (Wen et al, 1999), at the time of writing, the genus Comamonas comprised 19 species with validly published names (http://www.bacterio.net/comamonas.html; Hatayama, 2014). The strains affiliated with the genus Comamonas have been isolated from various environments, such as soil, food waste compost, activated sludge, termite gut and sulfur spring (Sun et al, 2013).…”