Colwellia marinimaniae sp. nov., a hyperpiezophilic species isolated from an amphipod within the Challenger Deep, Mariana Trench

Kusube, Masataka; Kyaw, Than S.; Tanikawa, Kumiko; Chastain, Roger A.; Hardy, Kevin; Cameron, James; Bartlett, Douglas H.

doi:10.1099/ijsem.0.001671

Cited by 64 publications

(39 citation statements)

References 47 publications

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“…We first evaluated the influence of high hydrostatic pressure on the growth of the seven 172 strains of Colwellia. The growth characteristics of Colwellia marinimaniae MT41, C. 173 marinimaniae MTCD1, and C. piezophila have been previously reported, showing growth 174 optima at 103 MPa (Yayanos, 1986;DeLong et al, 1997), 120 MPa (Kusube et al, 2017), and 175 60 MPa (Nogi et al, 2004), respectively. Colwellia sp.…”

Section: General Characteristics 171mentioning

confidence: 72%

“…This strain displays an optimum growth pressure of 120 MPa and a growth range from 80 to 140 140 MPa, higher than the pressure found at full ocean depth. Based on 16S rRNA gene similarity 141 both strains MT41 and MTCD1 strains were determined to belong to the species Colwellia 142 marinimaniae (Kusube et al, 2017). Other psychropiezophiles within the genus include C. 143 hadaliensis (Deming et al, 1988) and C. piezophila (Nogi et al, 2004), isolated from the Puerto 144 Rico and Japan trenches, respectively.…”

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confidence: 99%

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Distinctive Gene and Protein Characteristics of Extremely PiezophilicColwellia

Peoples

Kyaw

Ugalde

et al. 2020

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Background 24The deep ocean is characterized by low temperatures, high hydrostatic pressures, and low 25 concentrations of organic matter. While these conditions likely select for distinct genomic 26 characteristics within prokaryotes, the attributes facilitating adaptation to the deep ocean are 27 relatively unexplored. In this study, we compared the genomes of seven strains within the genus 28Colwellia, including some of the most piezophilic microbes known, to identify genomic features 29 that enable life in the deep sea. 30 31 Results 32 Significant differences were found to exist between piezophilic and non-piezophilic strains of 33 Colwellia. Piezophilic Colwellia have a more basic and hydrophobic proteome. The piezophilic 34 abyssal and hadal isolates have more genes involved in replication/recombination/repair, cell 35 wall/membrane biogenesis, and cell motility. The characteristics of respiration, pilus generation, 36 and membrane fluidity adjustment vary between the strains, with operons for a nuo 37 dehydrogenase and a tad pilus only present in the piezophiles. In contrast, the piezosensitive 38 members are unique in having the capacity for dissimilatory nitrite and TMAO reduction. A 39 number of genes exist only within deep-sea adapted species, such as those encoding d-alanine-d-40 alanine ligase for peptidoglycan formation, alanine dehydrogenase for NADH/NAD + 41 homeostasis, and archaeal methyltransferase for tRNA modification. Many of these piezophile-42 specific genes are in variable regions of the genome near genomic islands, transposases, and 43 toxin-antitoxin systems. 44 45 Conclusions 46We identified a number of adaptations that may facilitate deep-sea radiation in members of the 47 genus Colwellia, as well as in other piezophilic bacteria. An enrichment in more basic and 48 hydrophobic amino acids could help piezophiles stabilize and limit water intrusion into proteins 49 as a result of high pressure. Variations in genes associated with the membrane, including those 50 involved in unsaturated fatty acid production and respiration, indicate that membrane-based 51 adaptations are critical for coping with high pressure. The presence of many piezophile-specific 52 genes near genomic islands highlights that adaptation to the deep ocean may be facilitated by 53 horizontal gene transfer through transposases or other mobile elements. Some of these genes are 54 amenable to further study in genetically tractable piezophilic and piezotolerant deep-sea 55 microorganisms.

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Section: General Characteristics 171mentioning

confidence: 72%

mentioning

confidence: 99%

Distinctive Gene and Protein Characteristics of Extremely PiezophilicColwellia

Peoples

Kyaw

Ugalde

et al. 2020

Preprint

Self Cite

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“…nov. (Sun et al 2019); descriptions of these have recently been published. Nine other novel species have also been identified from the Mariana Trench (Hu et al 2015;Kusube et al 2017;Meng et al 2019;Pathom-aree et al 2006aPathom-aree et al , b, c, 2007Takai et al 1999;Wei et al 2018). This shows that the Mariana Trench contains abundant novel microbial resources and further study is bound to identify many more.…”

Section: Highly Diverse Heterotrophic Bacteria Were Obtained From Thementioning

confidence: 90%

Diversity of culturable heterotrophic bacteria from the Mariana Trench and their ability to degrade macromolecules

Zhao

Liu

Zhou

et al. 2020

Mar Life Sci Technol

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The Mariana Trench is the deepest location on earth and harbors unique microbial communities as evidenced by 16S rRNA gene amplicon and metagenomic sequencing. Obtaining culturable microorganisms from the Mariana Trench will contribute to a further understanding of hadal biogeochemical processes and act as a unique microbial reservoir with potential applications. Here, 825 bacterial strains, identified by 16S rRNA gene sequencing, were isolated from 12 water depths (0-10,400 m) of the Mariana Trench with 2216E and R2A media at 4 °C or 28 °C on four cruises during 2015-2017. These bacteria belong to four phyla, nine classes, 27 orders, 45 families and 108 genera. Alphaproteobacteria, Gammaproteobacteria, Actinobacteria_c, Bacilli and Flavobacteriia were the most abundant classes, accounting for 37.9%, 33.0%, 11.8%, 8.6% and 8.0% of the total bacterial isolates, respectively. 2216E and R2A media were found to have a better selectivity to Bacilli and Flavobacteriia, respectively. Fifty strains were potential novel bacterial species with a 16S rRNA gene similarity < 98.65%, and a higher percentage of novel strains were obtained from R2A than 2216E medium. Additionally, 301 (150 species) out of 354 strains (178 species) selected from each depth could degrade at least one of the ten kinds of macromolecules tested. These results indicate that there is a high diversity of culturable bacteria in the Mariana Trench and they can produce a variety of extracellular enzymes. Our study provides a valuable resource of microorganisms for investigating their biogeochemical roles in the Mariana Trench and for industrial applications.

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“…Some obligatory piezophiles which can optimally grow under 100 MPa are found in the deep sea. 40) However, growth of most miscellaneous microbes are suppressed at a MHHP of 60.8 MPa (600 atm), 41) although some lactic acid bacteria and heat-resistant bacteria can grow. 42) Meanwhile, high concentration of salt may inhibit enzyme activities in food and thus fermentation of salty products such as soy sauce and miso (Japanese fermented soybean paste) requires months of fermentation and maturation periods.…”

Section: Ivii Physical Changes In Food Componentsmentioning

confidence: 99%

Food processing by high hydrostatic pressure

Yamamoto

2017

Bioscience, Biotechnology, and Biochemistry

134

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High hydrostatic pressure (HHP) process, as a nonthermal process, can be used to inactivate microbes while minimizing chemical reactions in food. In this regard, a HHP level of 100 MPa (986.9 atm/1019.7 kgf/cm 2) and more is applied to food. Conventional thermal process damages food components relating color, flavor, and nutrition via enhanced chemical reactions. However, HHP process minimizes the damages and inactivates microbes toward processing high quality safe foods. The first commercial HHP-processed foods were launched in 1990 as fruit products such as jams, and then some other products have been commercialized: retort rice products (enhanced water impregnation), cooked hams and sausages (shelf life extension), soy sauce with minimized salt (short-time fermentation owing to enhanced enzymatic reactions), and beverages (shelf life extension). The characteristics of HHP food processing are reviewed from viewpoints of nonthermal process, history, research and development, physical and biochemical changes, and processing equipment.

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Colwellia marinimaniae sp. nov., a hyperpiezophilic species isolated from an amphipod within the Challenger Deep, Mariana Trench

Cited by 64 publications

References 47 publications

Distinctive Gene and Protein Characteristics of Extremely PiezophilicColwellia

Distinctive Gene and Protein Characteristics of Extremely PiezophilicColwellia

Diversity of culturable heterotrophic bacteria from the Mariana Trench and their ability to degrade macromolecules

Food processing by high hydrostatic pressure

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