Column parameters controlling resolution in high-performance gel permeation chromatography

Unger, Klaus K.; Kern, Rainer

doi:10.1016/s0021-9673(00)82257-8

Cited by 20 publications

(3 citation statements)

References 11 publications

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“…Both V IS and V o * decrease as the column temperature is raised. The decrease of V IS with temperature is believed to be mainly due to the thermal expansion of mobile phase. , V o * appears to show a stronger temperature dependence in the bare silica column than V IS , which seems to reflect an additional temperature effect on V o *.…”

Section: Resultsmentioning

confidence: 88%

Martin’s Rule for High-Performance Liquid Chromatography Retention of Polystyrene Oligomers

2009

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The liquid chromatographic retention of polystyrene (PS) oligomer was examined taking the column hold-up volume as an adjustable parameter based on the assumption that Martin's rule is valid, i.e., ln k (retention factor) is proportional to the degree of polymerization (DP). In both bare silica and C18-bonded silica columns, thus determined column hold-up volume is systematically smaller than the elution volume of the injection solvent. When the column hold-up volume was taken as an adjustable parameter, the retention of PS oligomer in organic mobile phase follows Martin's rule (ln k proportional, variant DP) near perfectly. In addition, the enthalpy and entropy changes associated with the solute transfer, determined from the temperature dependence of the PS oligomer retention, also show excellent linear dependence on the degree of polymerization if the adjustable column hold-up volume was used. When we compare the retention behavior of PS and hydroxyl terminated PS, the repeating (styrene) unit exhibits the same enthalpy and entropy change while the end group shows the corresponding difference. All observations conform to Martin's rule regardless of the columns used, but the physical nature of the adjustable column hold-up volume remains to be elucidated.

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Section: Resultsmentioning

confidence: 88%

Martin’s Rule for High-Performance Liquid Chromatography Retention of Polystyrene Oligomers

2009

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“…Thus, only fragments smaller than this could be trapped and probably is not of concern for most applications. Device parameters such as porosities and total surface area can be estimated using the physical properties of silica as well as the specifications of the silica resins as provided by the manufacturer (see Materials and Methods for details)-details on these relationships can be found in the literature (51,52). The DNA-accessible surface area for our SPE devices were determined to be 191 cm 2 for a device packed with 200 g of Merck G-60 resin and 84 cm 2 for a device packed with 210 g of Hyperprep material.…”

Section: Quantitative Evaluation Of Different Silica Particles: Spe Omentioning

confidence: 99%

Evaluation of Silica Resins for Direct and Efficient Extraction of DNA from Complex Biological Matrices in a Miniaturized Format

Tian

Hühmer

Landers

2000

Analytical Biochemistry

241

204

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“…Mesoporous silicas such as MCM-41, MCM-48, FSM-16, APMS, and MSU-1 have been prepared in the form of spherical particles and evaluated in normal-phase or reverse-phase HPLC. − However, there have been relatively few studies on the use of mesoporous silicas in SEC, and limited separation capacity was reported. , This is because most of the mesoporous silicas are limited in pore size (<8 nm) and pore volume (<1.5 cm 3 /g), rendering them unsuitable for separating bulky molecules (e.g., polymers) based on the size exclusion principle. Unger and Kern have studied the use of high-porosity amorphous silicas in SEC, but their columns suffered from relatively low selectivity and column efficiency due to the broad pore size distribution and large particle size of the packing material . Among the supramolecular-templated mesoporous silicas, mesocellular foam (MCF) templated by oil-in-water microemulsions seems to be the most suitable packing material for SEC because of its ultralarge pore size (20−50 nm), high pore volume (up to 2.6 cm 3 /g), and robust framework …”

Section: Introductionmentioning

confidence: 99%

Spherical Siliceous Mesocellular Foam Particles for High-Speed Size Exclusion Chromatography

2007

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Spherical micrometer-sized siliceous mesocellular foam (MCF) particles with narrow window size distributions and ultralarge pore volumes were synthesized and used as packing materials for size exclusion chromatography (SEC). MCF columns exhibited excellent size exclusion properties in separating polystyrenes in organic media. The window size of spherical MCF could be tailored between 10 and 26 nm without affecting the particle morphology, allowing this packing material to be used for analyzing different molecular weight ranges. Compared to a conventional SEC system consisting of two polymerbased columns, a single, smaller MCF column could achieve similarly high resolution with significantly reduced analysis time and solvent consumption. The MCF column also exhibited better separation capability than a commercial silica-based SEC column of the same dimensions. These advantages could be attributed to the ultrahigh porosity of MCF materials. Spherical MCF particles were demonstrated to be an excellent packing material for SEC, especially for high-speed applications.

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Column parameters controlling resolution in high-performance gel permeation chromatography

Cited by 20 publications

References 11 publications

Martin’s Rule for High-Performance Liquid Chromatography Retention of Polystyrene Oligomers

Martin’s Rule for High-Performance Liquid Chromatography Retention of Polystyrene Oligomers

Evaluation of Silica Resins for Direct and Efficient Extraction of DNA from Complex Biological Matrices in a Miniaturized Format

Spherical Siliceous Mesocellular Foam Particles for High-Speed Size Exclusion Chromatography

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