Column liquid chromatography–electrospray ionisation–tandem mass spectrometry for the analysis of ochratoxin

Becker, M.; Degelmann, Petra; Herderich, Markus; Schreier, Peter; Humpf, Hans‐Ulrich

doi:10.1016/s0021-9673(98)00594-9

Cited by 59 publications

(37 citation statements)

References 13 publications

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“…The product ion showing the highest intensity was used for quantification, m/z 239, which corresponded to the loss of phenylalanine for OTA. 8 The experimental results were checked for the presence of outliers through Grubbs test before the validation process, and none of the results were considered an outlier.…”

Section: Resultsmentioning

confidence: 99%

“…[2][3][4][5][6] This mycotoxin has been found in food commodities such as cereals, oleaginous seeds, wine, meat, cocoa, spices, dried fruits, grapes, beer, green, roasted and instant coffee. [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] Once OTA is formed, it survives in most of food-processing steps, such as cooking, fermenting and roasting, as in the case of coffee beans. Roasted coffee produces the most different results in terms of OTA stability, since losses were reported in the range of 0-100%.…”

Section: Introductionmentioning

confidence: 99%

“…The major disadvantage of this technique is the comparable low sensitivity towards OTA (ppb range) and frequently interferences with the sample matrix. 6,8,32 GC-based methods found distinctly less attention since they suffer from a time-consuming and error-prone derivatisation protocol (methylation), needed to achieve sufficient volatility of the analytes. Nevertheless, GC/MS can be used to confirm unambiguously positive findings.…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS)

et al. 2012

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Recebido em 19/1/11; aceito em 21/5/11; publicado na web em 22/7/11 A method using LC/ESI-MS/MS for the quantitative analysis of Ochratoxin A in roasted coffee was described. Linearity was demonstrated (r = 0.9175). The limits of detection and quantification were 1.0 and 3.0 ng g -1 , respectively. Trueness, repeatability and intermediate precision values were 89.0-108.8%; 2.4-13.7%; 12.5-17.8%, respectively. To the best of our knowledge, this is the first report in which Ochratoxin A in roasted coffee is analysed by LC/ESI-MS/MS, contributing to the field of mycotoxin analysis, and it will be used for future production of Certified Reference Material.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS)

et al. 2012

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“…The first work with modern instrumentation can be attributed to Becker et al (1998), who used an LC/ESI-MS/MS method for the quantification of OTA in wheat, coffee, and beer. The molecule was analyzed in the positive ion mode, where the fragmentation of the protonated molecular ion (m/z 404) yielded fragments corresponding to the loss of water (m/z 386), water/ carbon monoxide (m/z 358), phenylalanine (m/z 239), and water/ ammonia/carbon monoxide (m/z 341).…”

Section: A Liquid Chromatography/mass Spectrometry Determination Of mentioning

confidence: 99%

Recent advances in mycotoxin determination in food and feed by hyphenated chromatographic techniques/mass spectrometry

Sforza

Dall’Asta

Marchelli

2005

Mass Spectrometry Reviews

195

104

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Mycotoxins are fungal toxins produced by molds, which occur universally in food and feed derivatives, and are produced under certain environmental conditions in the field before harvest, post‐harvest, during storage, processing, and feeding. Mycotoxin contamination is one of the most relevant and worrisome problem concerning food and feed safety because it can cause a variety of toxic acute and chronic effects in human and animals. In this review we report the use of mass spectrometry in connection with chromatographic techniques for mycotoxin determination by considering separately the most diffuse class of mycotoxins: patulin, aflatoxins, ochratoxin A, zearalenone, trichothecenes, and fumonisins. Although the selectivity of mass spectrometry is unchallenged if compared to common GC and LC detection methods, accuracy, precision, and sensitivity may be extremely variable concerning the different mycotoxins, matrices, and instruments. The sensitivity issue may be a real problem in the case of LC/MS, where the response can be very different for the different ionization techniques (ESI, APCI, APPI). Therefore, when other detection methods (such as fluorescence or UV absorbance) can be used for the quantitative determination, LC/MS appears to be only an outstanding confirmatory technique. In contrast, when the toxins are not volatile and do not bear suitable chromophores or fluorophores, LC/MS appears to be the unique method to perform quantitative and qualitative analyses without requiring any derivatization procedure. The problem of exact quantitative determination in GC/MS and LC/MS methods is particularly important for mycotoxin determination in food, given the high variability of the matrices, and can be solved only by the use of isotopically labeled internal standards or by the use of ionization interfaces able to lower matrix effects and ion suppressions. When the problems linked to inconstant ionization and matrix effects will be solved, only MS detectors will allow to simplify more and more the sample preparation procedures and to avoid clean‐up procedures, making feasible low‐cost, high‐throughput determination of mycotoxins in many different food matrices. © 2005 Wiley Periodicals, Inc.

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“…In order to control AFs levels in agricultural products, several analytical methods have been developed TLC 9 , HPLC with different detectors [10][11][12][13] and enzyme linked immunosorbent assay 14 are the most commonly used. These methods are usually time consuming and require harmful solvents and well equipped laboratories.…”

Section: Introductionmentioning

confidence: 99%

Determination of Aflatoxins G and GUsing Ion Mobility Spectrometry

Sheibani¹,

Zare-Khormizi²,

Tabrizchi³

2012

Orientjchem.

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This work describes a rapid and sensitive ion mobility spectrometry method for the determination of aflatoxins G 1 and G 2 (AFG 1 and AFG 2 ). The effective instrumental parameters were investigated and optimized. After optimizing, the calibration curves for AFG 1 and AFG 2 were linear in the range of 1 to 300 ng. Relative standard deviation was 8% and limit of detection was 0.5 ng. The capability of the proposed method was evaluated for the determination of AFG in spiked pistachio nut as a real sample that satisfactory results were obtained.Key words: Aflatoxin G (AFG), Aflatoxin B (AFB), Ion mobility spectrometry (IMS), Pistachio nut.Some reports indicate natural contamination with AFB and AFG mainly in countries with warm and humid climate [6][7][8] .

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Column liquid chromatography–electrospray ionisation–tandem mass spectrometry for the analysis of ochratoxin

Cited by 59 publications

References 13 publications

Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS)

Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS)

Recent advances in mycotoxin determination in food and feed by hyphenated chromatographic techniques/mass spectrometry

Determination of Aflatoxins G and GUsing Ion Mobility Spectrometry

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