“…oxidation of sulphide minerals(Zou et al, 2015;Liu et al, 2016a) and enhance chalcocite, digenite and covellite leaching(Bolorunduro, 1999;Ruan et al, 2013). The high pyrite fraction also releases heat and sulphuric acid and iron (50 g/L) once it is oxidised(Ruan et al, 2006;Liu et al, 2010;Ruan et al, 2011Ruan et al, , 2013;Zou et al, 2015).Microbial genera present in the Zijinshan copper mine and solution ponds are identified as Acidithiobacillus (such as A. thiooxidans, A. albertensis and A. caldus, these are major sulphur oxidisers), Leptospirillum (ferrous oxidisers), Sulfobacillus, Acidiphilium and Ferrimicrobium Liu et al (2010Liu et al ( , 2016a. discussed this aspect in detail.…”