Column Chromatography and Cell Culture Assay of Pseudomonas aeruginosa Toxin Z Preparations

Ludovici, Peter P.; Roinestad, Frank A.; Wong, Fu-Lung

doi:10.1128/aem.30.2.293-297.1975

Cited by 2 publications

(1 citation statement)

References 4 publications

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“…The partially purified Z toxin was differentiated from the A toxin on the basis of its thermostability at 70 C (14). Figure 1 shows that the inactivating factor can reduce the ADPR-transferase activity of the PA toxin at temperatures as low as 30 C in a comparatively short period of time (30 min).…”

Section: Itmentioning

confidence: 99%

Temperature-dependent inactivating factor of Pseudomonas aeruginosa exotoxin A

Vasil¹,

Liu²,

Iglewski³

1976

Infect Immun

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The adenosine diphosphate ribosyl transferase activity of Pseudomonas aeruginosa exotoxin A (PA toxin) was found to be rapidly destroyed by heating at 45 to 60 C but not by heating at 70 to 90 C (for at least 30 min). This phenomenon has been previously described for other bacterial toxins (staphylococcal alpha-toxin and Vibrio parahaemolyticus hemolysin) and is termed an Arrhenius effect. In contrast, the Arrhenius effect was not seen when the PA toxin was heat-treated as above and tested for cell toxicity or mouse lethality. Although the PA toxin treated at 70 C for 30 min retained a significant proportion (>70%) of its adenosine diphosphate ribosyl transferase activity, the cell toxicity and mouse lethality of the toxin were virtually abolished. A temperature-dependent inactivating factor that has proteolytic activity and is co-purified with the PA toxin was shown to be responsible for the Arrhenius effect. PA toxin separated from the factor by conventional disc gel electrophoresis or PA toxin preparations lacking the factor did not show the Arrhenius effect.

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Section: Itmentioning

confidence: 99%