Columbia Blood Agar Base as an Alternative Medium for the Elek Test

Wang, Li; Nugroho, Febri Setyo; Herawati, Maria Holly

doi:10.5812/jjm-121537

Cited by 1 publication

(1 citation statement)

References 8 publications

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“…A thinner medium with less density and a closer distance between the antitoxin disc and inoculum reduced the time to obtain the results of the test to within 16 to 24 h. Moreover, the authors suggested that extending the incubation time may result in the occurrence of non-specific lines of precipitation. Further modifications proposed in the scientific literature concerned the medium used for the test, for example, the application of Columbia blood agar base instead of Elek base medium [ 41 ] and the application of a modified medium for simultaneous Cystinase and Elek tests [ 42 ].…”

Section: Immunological Assaysmentioning

confidence: 99%

Challenges of Diphtheria Toxin Detection

Prygiel,

Mosiej,

Polak

et al. 2024

Toxins

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Diphtheria toxin (DT) is the main virulence factor of Corynebacterium diphtheriae, C. ulcerans and C. pseudotuberculosis. Moreover, new Corynebacterium species with the potential to produce diphtheria toxin have also been described. Therefore, the detection of the toxin is the most important test in the microbiological diagnosis of diphtheria and other corynebacteria infections. Since the first demonstration in 1888 that DT is a major virulence factor of C. diphtheriae, responsible for the systemic manifestation of the disease, various methods for DT detection have been developed, but the diagnostic usefulness of most of them has not been confirmed on a sufficiently large group of samples. Despite substantial progress in the science and diagnostics of infectious diseases, the Elek test is still the basic recommended diagnostic test for DT detection. The challenge here is the poor availability of an antitoxin and declining experience even in reference laboratories due to the low prevalence of diphtheria in developed countries. However, recent and very promising assays have been developed with the potential for use as rapid point-of-care testing (POCT), such as ICS and LFIA for toxin detection, LAMP for tox gene detection, and biosensors for both.

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Section: Immunological Assaysmentioning

confidence: 99%