Coloured and low conductive fabrics by in situ laccase-catalysed polymerization

Abstract

Coloured and conductive fabrics were obtained through "in situ" laccase polymerization of catechol and pphenylenediamine under high-pressure homogenization. Both monomers, catechol and p-phenylenediamine, were polymerized by different laccase forms, namely native, PEGylated and Epoxy-PEGylated. All the catalysts were placed inside a textile fabric bag which served simultaneously as enzyme support and as substrate for coating with the newly produced polymers. The PEGylated laccase forms gave rise to a higher amount of oligomers/polymers and higher colouration level of polyethylene terephthalate (PET), cotton and wool fabrics compared to native laccase. Both functional polymers were able to confer conductivity to the substrates however in a different extent. Fabrics coated with poly(p-phenylenediamine) present higher conductivity, rather due to its polymerized structure than to the amount of polymer produced by enzyme catalysis. Herein a green approach was presented to produce polyphenols with increased fixation onto different textile substrates. These substrates reach high levels of colouration and good fastness behaviour after washing.

Antimicrobial coating of textiles by laccase in situ polymerization of catechol and p-phenylenediamine

“…Previous findings reveal that laccase, native or chemically modified, is able to efficiently oxidize phenolic compounds such as catechol and p-phenylenediamine monomers [20]. Our data showed that, although the role of PEG in enzymatic polymerization has not been clearly understood, the PEGylated enzyme form was more prone to produce higher amount and longer polymers than the native enzyme form [21].…”

“…Laccase from M. thermophila was PEGylated as previously reported [21] using the procedure of Daly et al [22]. -assisted polymerization of catechol and pphenylenediamine Catechol and p-phenylenediamine polymerization was processed by the method reported previously [20] by incubating 50 mM of each monomer with PEGylated laccase (100 U/mL) in acetate buffer (pH = 5). The enzyme was confined in bags of polyethylene glycol (PET), cotton, and wool, and the bags were placed in the sample receptor of the HPH device.…”

Applications of Microbial Laccases: Patent Review of the Past Decade (2009–2019)

“…Since then, numerous studies were published, reporting the synthesis of hair dyes [63], phenoxazine derivatives [166], benzene-and naphthalene-derived non-toxic dyes [167], 2,5-diaminobenzenesulfonic acid (2,5-DABSA) and catechol [67] or resorcinol [168] coupling derivatives, several aminoindamine and indoaniline dyes for hair dyeing and cosmetics applications [169], and even a dye for protein gel staining [170]. Moreover, the dyeing of fabrics by the in situ polymerization of phenol and amine compounds has been reported [115,171]. These are also covered in patents dealing with the preparation of paper-based lacquer paintings (CN109627825A), oxygen scavenger coating composition (US2009226996A1), or thermal insulation coatings for exterior walls (CN109735175A)).…”

Laccases as versatile enzymes: from industrial uses to novel applications

“…developed an in situ enzyme dyeing method (polymerization of DABSA with T. versicolor laccase) obtaining wool fabrics with special pH‐responsive, color‐changing and conductive properties. Su et al . coated textile fabrics (cotton, wool, and polyethylene terephthalate) with poly(catechol) and poly( p ‐phenylenediamine) using native and PEGylated M. thermophila laccase.…”