Colour variation in red grapevines (Vitis vinifera L.): genomic organisation, expression of flavonoid 3'-hydroxylase, flavonoid 3',5'-hydroxylase genes and related metabolite profiling of red cyanidin-/blue delphinidin-based anthocyanins in berry skin

Castellarin, Simone D.; Gaspero, Gabriele Di; Marconi, Raffaella; Nonis, Alberto; Peterlunger, Enrico; Paillard, Sophie; Adam-Blondon, Anne-Françoise; Testolin, R.

doi:10.1186/1471-2164-7-12

Cited by 211 publications

(140 citation statements)

References 41 publications

(35 reference statements)

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“…1, 2). The continuous distribution of anthocyanins here in our study is in accord with reports by Lijavetzky et al (2006) and Castellarin et al (2006) who used molecular techniques to show that anthocyanin content in grape was a quantitative character controlled by polygenes.…”

Section: Discussionsupporting

confidence: 91%

“…If breeding wine grapes, a high tartaric acid parent should be chosen. With the development of biotechnology and a completed grape genome sequence, genetic analyses are progressing (Firoozabady and Olmo 1987;Ibáñez et al 2003;Castellarin et al 2006;Lijavetzky et al 2006;Jaillon et al 2007). However, these analyses are still difficult in grape due to a long growth period, large chromosome numbers, partial sterility of ovules, and low seed germination rates (He 1999).…”

Section: Introductionmentioning

confidence: 98%

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Inheritance of anthocyanins in berries of Vitis vinifera grapes

Liang

Yang

et al. 2009

Euphytica

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Inheritance patterns for table grape anthocyanins were investigated on three cross offspring populations during two successive years. Sixteen anthocyanins were detected, and all were monoglucoside derivatives. The proportion of anthocyanins in the maternal parent determined the proportion of anthocyanins in the offspring. But the absolute content of the maternal parent had no significant effect on progenies. Peonidin 3-O-glucoside and malvidin 3-O-glucoside were the most abundant anthocyanins, not only in the maternal parent but also in the progenies. The presence or absence of anthocyanins in grape skin was inheritance of a quality character controlled by oligogenes, and anthocyanins content was a quantitative character controlled by polygenes. Via principal component (PC) analysis, factors that affected the total content of cross progeny populations were peonidin 3-O-glucoside, malvidin 3-O-glucoside, delphinidin 3-O-glucoside, cyanidin 3-O-glucoside, petunidin 3-O-glucoside, peonidin 3-O-(6-O-coumaryl)-glucoside, and malvidin 3-O-(6-O-coumaryl)-glucoside. Anthocyanins content was a high broad sense heritability character (H 2 ), and H 2 was stable in different cross combinations (ranging from 0.65 to 0.98).

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 98%

Inheritance of anthocyanins in berries of Vitis vinifera grapes

Liang

Yang

et al. 2009

Euphytica

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“…Such changes in hydroxylation of the B-ring of flavonoids may be determined by the relative activities and substrate specificity of F 3 0 -H and F 3 0 5 0 -H, Castellarin et al 2006). F 3 0 5 0 -H belong to a large multigenic family in the grapevine genome (Jaillon et al 2007;Velasco et al 2007), each one being probably regulated in a precise time and tissue specific manner Jeong et al 2006).…”

Section: Overexpression Of Vlmyba1-2 Induces Anthocyanin Pigmentationmentioning

confidence: 99%

Ectopic expression of VlmybA1 in grapevine activates a narrow set of genes involved in anthocyanin synthesis and transport

et al. 2008

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The colour of the red wine is essentially due to the release of anthocyanins from the red skin of grape berries during the process of wine making. Anthocyanins are synthesized during ripening of the berries under the control of VvMYBA1 transcription factor that controls the expression of UFGT. In order to identify the whole set of downstream regulated genes, we targeted constitutive ectopic expression of VlmybA1-2 into grapevine hairy roots and plants. The ectopic expression of VlmybA1-2 triggered de novo production and storage of anthocyanins in all transgenic vegetative organs, leading to a very intense red coloration, and did not interfere with proanthocyanidin (PA) biosynthesis. The ectopic red pigmentation was due to the accumulation of anthocyanins in vacuoles and anthocyanin vacuolar inclusion (AVIs) in all organs but only in specific tissues. A transcriptomic analysis using a 14 K oligoarray revealed that the ectopic expression of VlmybA1-2 activated only few genes, most of which are involved in both PA and anthocyanin biosynthesis, while the expression of BAN and LAR (two specific genes of the PA biosynthesis pathway) was unaffected. Among these, 4 genes emerged given the amplitude of their up-regulation, quantitatively similar to VlmybA1-2 itself. In addition to the previously described UFGT, this set comprised an isogen of GST, an O-methyltransferase, both of which are supposed to play a role in the anthocyanin biosynthesis pathway, as well as a candidate gene putatively involved in the vacuolar anthocyanin transport in grapevine (anthoMATE). Together, these results suggest that MybA1 activates the last steps of anthocyanin synthesis and transport through the regulation of a narrow, specific spectrum of genes regulated as a cluster.

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“…However, the nonanthocyanin polyphenolic composition of the leaves of these varieties is unstudied. Since genetic differences among phenotypes may render the importance of environmental factors on phenolics production moot [25], these varieties offer an amenable model to determine the importance of genetic and environmental factors influencing grapevine phenolic composition. Nevertheless, the native varieties bring diversity; therefore, their characterization is critical.…”

Section: Introductionmentioning

confidence: 99%

Main Leaf Polyphenolic Components of Berry Color Variant Grapevines and Their Acclimative Responses to Sunlight Exposure

Kocsis

Abrankó²,

Ayaydin³

et al. 2015

Applied Sciences

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Grapevine leaf synthesizes a wide variety of bioactive secondary metabolites, including polyphenols, which are also key components in ensuring development and growth of the whole plant even under adverse environmental conditions. Our study evaluates the nonanthocyanin polyphenolic composition in grapevine leaves of three varieties of Gohér conculta (Vitis vinifera L.) native to Hungary. A high performance liquid chromatography (HPLC) system including a diode array detector (DAD) coupled to a time-of-flight mass spectrometer (q-TOFMS) was successfully applied to profile intact glycoconjugate forms in samples. In-source fragmentation was utilized in order to provide structural information on the compounds. Using this method, the presence of 16 polyphenolic metabolites were confirmed, and eight of them were subjected to further quantification in sun acclimated and half shaded leaves. Intracellular microimaging detected accumulation of flavonols in cell nuclei, cell wall and chloroplasts. Our findings demonstrated that Gohér conculta-a special grapevine taxon of our viticultural heritage with berry color variants-is a suitable model to OPEN ACCESSAppl. Sci. 2015Sci. , 5 1956 study the interaction between genetic and environmental factors in determination of grapevine phenolic composition.

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Colour variation in red grapevines (Vitis vinifera L.): genomic organisation, expression of flavonoid 3'-hydroxylase, flavonoid 3',5'-hydroxylase genes and related metabolite profiling of red cyanidin-/blue delphinidin-based anthocyanins in berry skin

Cited by 211 publications

References 41 publications

Inheritance of anthocyanins in berries of Vitis vinifera grapes

Inheritance of anthocyanins in berries of Vitis vinifera grapes

Ectopic expression of VlmybA1 in grapevine activates a narrow set of genes involved in anthocyanin synthesis and transport

Main Leaf Polyphenolic Components of Berry Color Variant Grapevines and Their Acclimative Responses to Sunlight Exposure

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