ur interest in molecular variation at the population level prompted isolation of large numbers of Eupenicillium strains from several tropical soils. These strains were all originally identified as E. shearii, based on cultural characters and morphology of the anamorphic structures. However, ISSR fingerprinting indicated that we were actually dealing with two taxa, and subsequent phylogenetic analysis using ITS-LSU sequences showed that the two taxa were distinct species (TUTHILL, this issue). One of the species was clearly E. shearii, since the ex-type strain of that species was included in the cluster, but the other, larger group could not be assigned to any known species of Eupenicillium. An additional strain in our collection, from the Galapagos Islands, matched the new species in every respect. The new species is described here as Eupenicillium tropicum sp. nov.Forty-three strains of Eupenicillium tropicum sp. nov. were isolated from soils collected in India, Costa Rica and Galapagos, Ecuador. The species is characterized by biverticillate penicilli, slightly rough, subglobose to ovate conidia, brownish cleistothecia that become brown-gray with age, and ascospores with two equatorial flanges and slightly roughened valves. All strains produced a large number of indole alkaloids, and many types of unknown secondary metabolites with characteristic chromophores were produced by a majority of strains. Eupenicillium tropicum is morphologically most similar to E. shearii, but based on ITS-LSU sequences, is most closely related to Penicillium citrinum, P. sartoryi and P. westlingii. Eupenicillium shearii strains consistently produce paxillin, paspalinine and shearinins, while the latter three penicillia all produce citrinin consistently.