Colour morphotypes of Elysia timida (Sacoglossa, Gastropoda) are determined by light acclimation in food algae

Costa, José Lino; Casalduero, Francisca Giménez; Melo, Ricardo; Jesus, Bruno

doi:10.3354/ab00446

Cited by 11 publications

(20 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NDVI was established in the 1970s and has been one of the most widely used indices for estimating Chl a content in different organisms and different ecosystems. Besides its use in terrestrial plants, it has been successfully used in a range of aquatic organisms including microalgae-dominated biofilms (Serôdio et al 2009;Perkins et al 2010), photosymbiotic sea slugs Costa et al 2012), and macroalgae and seagrasses (Peñuelas et al 1993). Similarly to NDVI, Ralph et al (2005) have used the same principle to measure absorptivity of corals, also using red and IR light, but with a slightly different formula: absorptivity = 1À(red/IR).…”

Section: Chl a Contentmentioning

confidence: 99%

Concurrent imaging of chlorophyll fluorescence, Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians

et al. 2014

View full text Add to dashboard Cite

Research on photosynthetic cnidarians has been mainly focused on the symbiosis established between the cnidarian host and its dinoflagellates endosymbionts from genus Symbiodinium. Despite the potential of imaging techniques for assessing the spatial distribution of key parameters of cnidarian photobiology, such as photochemical activity, chlorophyll a content or green fluorescent proteins (GFPs), to our best knowledge, no study has ever attempted to simultaneous map these three features. In this study, we developed a modified imaging pulse amplitude fluorometer by applying excitation light of different wavelengths and selectively detecting short spectral bands through bandpass filters. The imaging system was used to sequentially excite and quantify chlorophyll variable fluorescence (maximum quantum yield of photosystem II, F v /F m ), Chl a content (normalized difference vegetation index) and relative content of GFPs. The spatial distribution of these photophysiological parameters was mapped both horizontally, across the surface of the soft corals Sarcophyton cf. glaucum and Sinularia flexibilis and the zoanthid Protopalythoa sp., and vertically, throughout a vertical section of S. cf. glaucum. Results showed bleached areas within each individual coral colony and registered photophysiological changes with S. cf. glaucum tissue depth. Analysis of Protopalythoa sp. polyps' expansion revealed differential surface patterns of NDVI and GFP concentration, and a negative relation between these latter parameters within each polyp. This novel non-invasive approach allowed a high-resolution characterization of the spatial relationship between these key parameters through the analysis of image information on a pixel-by-pixel basis, which has great potential for investigating the physiological state of symbiotic associations.

show abstract

Section: Chl a Contentmentioning

confidence: 99%

Concurrent imaging of chlorophyll fluorescence, Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians

et al. 2014

View full text Add to dashboard Cite

show abstract

“…B 369: 20130242 xanthophyll cycle is the main photoprotective mechanism in many photoautotrophs, including algae of various groups [29], and should be a natural topic for kleptoplasty research. Although the presence of xanthophyll cycle pigments in kleptoplasts has been demonstrated [13,16,17], its operation as a response to high light exposure has, thus far, only been shown for the E. timida/A. acetabulum association [15].…”

Section: Photoprotection and Photoinhibitionmentioning

confidence: 99%

“…The absence of photoacclimation in kleptoplasts is also consistent with the strong association between LCs of relative electron transport rate (rETR) of algal food and corresponding kleptoplastic hosts. A compilation of all published results [13,[15][16][17] and original data (S. Cruz, T. Santos, P. Cartaxana, R. Calado, J. Serô dio, unpublished; http://dx.doi.org/10. 6084/m9.figshare.783888) showed that despite the large range in algal prey photoacclimation state (as denoted by the light-response curve photoacclimation parameter E k , ranging from 28.7 to 356.0 mmol m 22 s…”

Section: Photoacclimationmentioning

confidence: 99%

Photophysiology of kleptoplasts: photosynthetic use of light by chloroplasts living in animal cells

Serôdio

Cruz

Cartaxana

et al. 2014

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

Kleptoplasty is a remarkable type of photosynthetic association, resulting from the maintenance of functional chloroplasts—the ‘kleptoplasts’—in the tissues of a non-photosynthetic host. It represents a biologically unique condition for chloroplast and photosynthesis functioning, occurring in different phylogenetic lineages, namely dinoflagellates, ciliates, foraminiferans and, most interestingly, a single taxon of metazoans, the sacoglossan sea slugs. In the case of sea slugs, chloroplasts from macroalgae are often maintained as intracellular organelles in cells of these marine gastropods, structurally intact and photosynthetically competent for extended periods of time. Kleptoplasty has long attracted interest owing to the longevity of functional kleptoplasts in the absence of the original algal nucleus and the limited number of proteins encoded by the chloroplast genome. This review updates the state-of-the-art on kleptoplast photophysiology, focusing on the comparative analysis of the responses to light of the chloroplasts when in their original, macroalgal cells, and when sequestered in animal cells and functioning as kleptoplasts. It covers fundamental but ecologically relevant aspects of kleptoplast light responses, such as the occurrence of photoacclimation in hospite , operation of photoprotective processes and susceptibility to photoinhibition. Emphasis is given to host-mediated processes unique to kleptoplastic associations, reviewing current hypotheses on behavioural photoprotection and host-mediated enhancement of photosynthetic performance, and identifying current gaps in sacoglossan kleptoplast photophysiology research.

show abstract

“…The latter parameter has been shown to be a good parameter to estimate PSII functionality (e.g. Vieira et al, 2009;Jesus et al, 2010;Serodio et al, 2010;Costa et al, 2012;Curtis et al, 2013;Ventura et al, 2013).…”

Section: T Jauffrais Et Al: Effect Of Light On Photosynthetic Efficmentioning

confidence: 99%

“…oxygen evolution by microelectrodes (Rink et al, 1998;Geslin et al, 2011) or 14 C radiotracer (Lopez, 1979). Recently, pulse amplitude modulated (PAM) fluorometry has been used extensively in the study of kleptoplastic sacoglossans (Vieira et al, 2009;Costa et al, 2012;Jesus et al, 2010;Serodio et al, 2010;Curtis et al, 2013;Ventura et al, 2013). This non-invasive technique has the advantage of estimating relative electron transport rates (rETR) using rapid light curves (RLC) and photosystem II (PSII) maximum quantum efficiencies (Fv/Fm) very quickly and without incubation periods.…”

Section: T Jauffrais Et Al: Effect Of Light On Photosynthetic Efficmentioning

confidence: 99%

Effect of light on photosynthetic efficiency of sequestered chloroplasts in intertidal benthic foraminifera (<i>Haynesina germanica</i> and <i>Ammonia tepida</i>)

et al. 2016

View full text Add to dashboard Cite

Abstract. Some benthic foraminifera have the ability to incorporate functional chloroplasts from diatoms (kleptoplasty). Our objective was to investigate chloroplast functionality of two benthic foraminifera (Haynesina germanica and Ammonia tepida) exposed to different irradiance levels (0, 25, 70 µmol photon m −2 s −1 ) using spectral reflectance, epifluorescence observations, oxygen evolution and pulse amplitude modulated (PAM) fluorometry (maximum photosystem II quantum efficiency (Fv/Fm) and rapid light curves (RLC)). Our results clearly showed that H. germanica was capable of using its kleptoplasts for more than 1 week while A. tepida showed very limited kleptoplastic ability with maximum photosystem II quantum efficiency (Fv/Fm = 0.4), much lower than H. germanica and decreasing to zero in only 1 day. Only H. germanica showed net oxygen production with a compensation point at 24 µmol photon m −2 s −1 and a production up to 1000 pmol O 2 cell −1 day −1 at 300 µmol photon m −2 s −1 . Haynesina germanica Fv/Fm slowly decreased from 0.65 to 0.55 in 7 days when kept in darkness; however, it quickly decreased to 0.2 under high light. Kleptoplast functional time was thus estimated between 11 and 21 days in darkness and between 7 and 8 days at high light. These results emphasize that studies about foraminifera kleptoplasty must take into account light history. Additionally, this study showed that the kleptoplasts are unlikely to be completely functional, thus requiring continuous chloroplast resupply from foraminifera food source. The advantages of keeping functional chloroplasts are discussed but more information is needed to better understand foraminifera feeding strategies.

show abstract

Colour morphotypes of Elysia timida (Sacoglossa, Gastropoda) are determined by light acclimation in food algae

Cited by 11 publications

References 24 publications

Concurrent imaging of chlorophyll fluorescence, Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians

Concurrent imaging of chlorophyll fluorescence, Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians

Photophysiology of kleptoplasts: photosynthetic use of light by chloroplasts living in animal cells

Effect of light on photosynthetic efficiency of sequestered chloroplasts in intertidal benthic foraminifera (<i>Haynesina germanica</i> and <i>Ammonia tepida</i>)

Contact Info

Product

Resources

About

Colour morphotypes of Elysia timida (Sacoglossa, Gastropoda) are determined by light acclimation in food algae

Cited by 11 publications

References 24 publications

Concurrent imaging of chlorophyll fluorescence, Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians

Concurrent imaging of chlorophyll fluorescence, Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians

Photophysiology of kleptoplasts: photosynthetic use of light by chloroplasts living in animal cells

Effect of light on photosynthetic efficiency of sequestered chloroplasts in intertidal benthic foraminifera (&lt;i&gt;Haynesina germanica&lt;/i&gt; and &lt;i&gt;Ammonia tepida&lt;/i&gt;)

Contact Info

Product

Resources

About

Effect of light on photosynthetic efficiency of sequestered chloroplasts in intertidal benthic foraminifera (<i>Haynesina germanica</i> and <i>Ammonia tepida</i>)