Colour-enhancing protein in blue petals

Fukada-Tanaka, Sachiko; Inagaki, Yoshishige; Yamaguchi, Toshio; Saitô, Norio; Iida, Shigeru

doi:10.1038/35036683

Cited by 173 publications

(101 citation statements)

References 7 publications

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“…We do not know how this 3-bp insertion occurred in the PnSCR at present, but we speculate that it is a footprint generated by an excision of a DNA transposon in the Tpn1 family belonging to the CACTA superfamily. In fact, the Tpn1-related elements are thought to act as major spontaneous mutagens for the generation of various floricultural traits in the Japanese morning glory and are shown to generate 3-bp insertions (27,28).…”

Section: Discussionmentioning

confidence: 99%

Shoot circumnutation and winding movements require gravisensing cells

Kitazawa

Hatakeda

Kamada

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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Circumnutation and winding in plants are universal growth movements that allow plants to survive despite their sessile nature. However, the detailed molecular mechanisms controlling these phenomena remain unclear. We previously found that a gravitropic mutant of Japanese morning glory (Pharbitis nil or Ipomoea nil), Shidare-asagao (weeping), is defective not only in circumnutation but also in the winding response. This phenotype is similar to that of the Arabidopsis SCARECROW (SCR) mutant. We therefore investigated whether morning glory SCR (PnSCR) is involved in the weeping phenotype. We found that one amino acid was inserted into the highly conserved VHIID motif in weeping-type PnSCR; this mutation caused abnormal endodermal differentiation. We introduced either the mutant or WT PnSCR into Arabidopsis scr mutants for complementation tests. PnSCR of the WT, but not of weeping, rescued the shoot gravitropism and circumnutation of scr. These results show that both the abnormal gravitropism and the circumnutation defect in weeping are attributable to a loss of PnSCR function. Thus, our data show that gravisensing endodermal cells are indispensable for shoot circumnutation and the winding response and that PnSCR is responsible for the abnormal phenotypes of weeping.Arabidopsis ͉ gravitropic mutant ͉ gravity sensing ͉ morning glory ͉ SCARECROW

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Section: Discussionmentioning

confidence: 99%

Shoot circumnutation and winding movements require gravisensing cells

Kitazawa

Hatakeda

Kamada

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…The yeast homolog ScNHX1, localized in the prevacuolar compartment, is required for endosomal protein trafficking (38), adaptation to acute hypoosmotic shock (19), and resistance to various toxic organic cations (8). Significantly, a mutation in a gene of Ipomoea nil most similar to AtNHX1 abrogated capacity to increase vacuolar pH, a requirement for corolla color transition (39), clearly indicating that plant NHX1-like proteins are instrumental to vacuolar pH regulation. Moreover, the AtNHX1 gene was not only induced in Arabidopsis by NaCl but also by treatment with KCl or the dehydration-related hormone ABA (8,17), thus suggesting a function of AtNHX1 in plant responses to changes in external osmolarity.…”

Section: Cation/h ϩ Transport By the Intracellular Exchanger Atnhx1 2416mentioning

confidence: 99%

The Arabidopsis Na+/H+Exchanger AtNHX1 Catalyzes Low Affinity Na+ and K+ Transport in Reconstituted Liposomes

Venema

Quintero

Pardo

et al. 2002

Journal of Biological Chemistry

204

153

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“…Engineering of the petunia avonol 3 0 -and 5 0 hydroxylase genes into carnations resulted in the light and dark mauve Moondust and Moonshadow carnations (36). The recent identi cation of a gene controlling vacuolar pH in the intensely blue Japanese morning glory may facilitate modulation of vacuolar pH to achieve a truly blue variety (37).…”

Section: Genetic Engineering Of Novel Pigments In Plantsmentioning

confidence: 99%

Exploiting the Versatility of Human Cytochrome P450 Enzymes: The Promise of Blue Roses From Biotechnology

Gillam

Guengerich²

2001

IUBMB Life

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SummaryThe cytochrome P450 (P450) enzymes involved in drug metabolism are among the most versatile biological catalysts known. A small number of discrete forms of human P450 are capable of catalyzing the monooxygenatio n of a practically unlimited variety of xenobiotic substrates, with each enzyme showing a more or less wide and overlapping substrate range. This versatility makes P450s ideally suited as starting materials for engineering designer catalysts for industrial applications. In the course of heterologous expression of P450s in bacteria, we observed the unexpected formation of blue pigments. Although this was initially assumed to be an artifact, subsequent work led to the discovery of a new function of P450s in intermediary metabolism and toxicology, new screens for protein engineering, and potential applications in the dye and horticulture industries.

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Colour-enhancing protein in blue petals

Cited by 173 publications

References 7 publications

Shoot circumnutation and winding movements require gravisensing cells

Shoot circumnutation and winding movements require gravisensing cells

The Arabidopsis Na+/H+Exchanger AtNHX1 Catalyzes Low Affinity Na+ and K+ Transport in Reconstituted Liposomes

Exploiting the Versatility of Human Cytochrome P450 Enzymes: The Promise of Blue Roses From Biotechnology

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