Colostrum Proteomics Research: A Complex Fluid with Multiple Physiological Functions

Hernández-Castellano, Lorenzo E.; Baumrucker, Craig R.; Gross, Josef Johann; Wellnitz, Olga; Bruckmaier, Rupert M.

doi:10.1007/978-3-319-69682-9_8

Cited by 3 publications

(2 citation statements)

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“…Our analysis mapped LC-MS/MS data to 772 proteins corresponding to 501 non-redundant protein-coding genes, and thus, at least relative to macaque milk, appeared to be as robust without pre-fractionation. Moreover, most of the proteins identified in sow milk samples overlapped with those reported to be found in cow and human milk ( Berg et al, 2002 ; Zhang et al, 2013 ; Hernández-Castellano et al, 2018 ; Fahey et al, 2020 ). Analysis of homogenate milk samples also proved to capture as many proteins that spanned a similar range in abundance as studies that fractionated milk to analyze whey or milk fat globule membrane proteins using LC-MS/MS.…”

Section: Discussionmentioning

confidence: 70%

“…Shotgun proteomic analysis of homogenate sow milk samples by LC-MS/MS enabled detection of 207 unique high confidence proteins across colostrum, transitional, and mature milk lactation phases. Most of the previous proteome studies, fractionated milk samples and examined proteins associated with milk fat globule membranes or whey-skim fractions Reinhardt et al, 2008 ; Zhang et al, 2013 ; Yang et al, 2016 ; Hernández-Castellano et al, 2018 ; Fahey et al, 2020 ). Although these approaches enabled an understanding of component milk proteomes, they precluded analysis of the relative abundance of proteins to each other and thus relative abundance available to neonates.…”

Section: Discussionmentioning

confidence: 99%

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Shotgun proteomics of homogenate milk reveals dynamic changes in protein abundances between colostrum, transitional, and mature milk of swine

Bradshaw

Trujillo

Luecke

et al. 2021

Journal of Animal Science

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Milk is an easily digestible source of nutrients and bioactive factors, its composition reflects the neonate's needs, and changes from colostrum to transitional and mature milk. Our objective was to measure milk fat, lactose, total carbohydrate, and protein content in parallel with global proteome of homogenate milk samples to characterize changes across the three phases of swine lactation. Milk samples were collected from multiparous sows (n=9) on postnatal day 0 (D0; colostrum), 3 (D3; early transitional), 7 (D7; late transitional) and 14 (D14; mature). On D3, percent fat (16 ± 2.1) and lactose (3.8 ± 0.3) were higher (P<0.05) than on D0 (10 ± 3.9, and 1.5 ± 0.3; respectively). Levels of fat and lactose were not different between D3 and D14. Percent total protein decreased (P<0.05) between D0 (11 ± 2.1) and D3 (5 ± 0.7), but there was no significant change in percent protein between D3 and D14. Total carbohydrates increased (P<0.05) between D3 (944 ± 353 µg/ml) and D14 (1150 ± 462 µg/ml). Quantitative proteomic analysis using liquid chromatography tandem mass spectrometry (LC-MS/MS) of homogenate D0, D3, and D14 milk samples (n=6) identified 772 protein groups which corresponded to 501 individual protein-coding genes. A total of 207 high confidence proteins were detected in n=3 sows/day. Of the high confidence proteins, 81 proteins were common amongst all three days of lactation. Among the proteins that decreased between the days (FDR < 0.05) were multiple apolipoproteins and XDH which decreased between D0 to D3. Proteins that increased across the days (FDR < 0.05) were complement factors and14-3-3 proteins (YWHAQ, YWHAE). Our data provide a good characterization of milk proteome changes that likely reflect mammary function as well as the neonate's phase-specific developmental needs. This data may be useful in developing approaches to enhance the health and welfare of swine.

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Section: Discussionmentioning

confidence: 70%