Colostrum protein uptake in neonatal lambs examined by descriptive and quantitative liquid chromatography-tandem mass spectrometry

Hernández-Castellano, Lorenzo E.; Argüello, A.; Almeida, André M.; Castro, N.; Bendixen, Emøke

doi:10.3168/jds.2014-8143

Cited by 42 publications

(31 citation statements)

References 74 publications

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“…In agreement with this, in the current experiment, protein and fat percentages decreased and the lactose percentage increased from partum to 10 d of lactation. However, once colostrum production ceased, the milk composition was almost constant over time because the mammary gland developed a high tolerance to external factors, mainly diet [30,31], over the course of evolution to preserve functions and ensure the survival of the newborn ruminants [32]. In addition, this resilience is extendable to the fatty acid composition [33].…”

Section: Milkmentioning

confidence: 99%

Effect of Rearing System on the Straight and Branched Fatty Acids of Goat Milk and Meat of Suckling Kids

Ripoll

Aldea

Argüello

et al. 2020

Foods

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Goat meat is considered healthy because it has fewer calories and fat than meat from other traditional meat species. It is also rich in branched chain fatty acids that have health advantages when consumed. We studied the effects of maternal milk and milk replacers fed to suckling kids of four breeds on the straight and branched fatty acid compositions of their muscle. In addition, the proximal and fatty acid compositions of colostrum and milk were studied. Goat colostrum had more protein and fat and less lactose than milk. Goat milk is an important source of healthy fatty acids such as C18:1 c9 and C18:2 n-6. Suckling kid meat was also an important source of C18:1c9. Dairy goat breeds had higher percentages of trans monounsaturated fatty acids (MUFAs) and most of the C18:1 isomers but lower amounts of total MUFAs than meat breeds. However, these dairy kids had meat with a lower percentage of conjugated linoleic acid (CLA) than meat kids. The meat of kids fed natural milk had higher amounts of CLA and branched chain fatty acids (BCFAs) and lower amounts of n-6 fatty acids than kids fed milk replacers. Both milk and meat are a source of linoleic, α-linolenic, docosahexaenoic, eicosapentaenoic and arachidonic fatty acids, which are essential fatty acids and healthy long-chain fatty acids.Foods 2020, 9, 471 2 of 19 who use autochthonous breeds, believe that early separation decreases both milk yield and growth of the kids. In addition, they also believe that meat from kids reared with milk replacers is tough [6] and, as a consequence, they are opposed to this practice. This belief could be explained because most of the kid meat with high pH, which may induce tough meat, comes from kids raised on milk replacers [7]. On the other hand, the meat of kids reared with milk replacers was preferred by consumers according to visual appraisal, and consequently, as the purchase intention of these consumers was high [8,9]. Another advantage of meat from suckling kids fed natural milk is that it has a high percentage of hexanal, which has been positively related to both the flavor and overall acceptability [10].On the other hand, goat meat is considered healthy because it has fewer calories and fat than meat from other meat species, such as pork or lamb [11] and because its fatty acid profile complies with the recommendation of the World Health Organization, which states that trans fatty acid intake should not reach more than 5% [12]. In addition, goat meat is rich in branched chain fatty acids (BCFAs), and BCFAs are of interest for two main reasons: first, BCFAs, particularly short chain BCFAs, have an impact on the characteristic flavor of meat and dairy products [13][14][15][16]. Second, some authors have described health advantages with BCFA consumption. Additionally, the intake of BCFAs is related to the correct function of the newborn gut [17] and the induction of apoptosis in breast cancer cells [18]. Considering these benefits, BCFAs could be considered bioactive compounds and deserve deep insight. BCFAs are mainly satura...

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Section: Milkmentioning

confidence: 99%

Effect of Rearing System on the Straight and Branched Fatty Acids of Goat Milk and Meat of Suckling Kids

Ripoll

Aldea

Argüello

et al. 2020

Foods

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“…Several studies have been previously reported, focusing primarily on the genome and on gene functions, nevertheless a growing number of the so-called post-genomics tools (Proteomics, Lipidomics, Metabolomics and Transcriptomics) have been playing an important role in understanding how biological systems work and interact. In particular, proteomics has been found to have a major role, not just in animal selection, but in diverse areas of animal and veterinary sciences [ 16 ][ 17 ] such as meat sciences [ 18 ][ 19 ], colostrum uptake [ 20 ][ 21 ][ 22 ] or the study of specific diseases such as chronic kidney disease in cats [ 23 ]. In fact, and although farm animal species poses some limitations [ 24 ], it is clear that significant results have been achieved in species such as cattle [ 25 ], rabbit [ 26 ] and pig [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

The Goat (Capra hircus) Mammary Gland Mitochondrial Proteome: A Study on the Effect of Weight Loss Using Blue-Native PAGE and Two-Dimensional Gel Electrophoresis

et al. 2016

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Seasonal weight loss (SWL) is the most important limitation to animal production in the Tropical and Mediterranean regions, conditioning producer’s incomes and the nutritional status of rural communities. It is of importance to produce strategies to oppose adverse effects of SWL. Breeds that have evolved in harsh climates have acquired tolerance to SWL through selection. Most of the factors determining such ability are related to changes in biochemical pathways as affected by SWL. In this study, a gel based proteomics strategy (BN: Blue-Native Page and 2DE: Two-dimensional gel electrophoresis) was used to characterize the mitochondrial proteome of the secretory tissue of the goat mammary gland. In addition, we have conducted an investigation of the effects of weight loss in two goat breeds with different levels of adaptation to nutritional stress: Majorera (tolerant) and Palmera (susceptible). The study used Majorera and Palmera dairy goats, divided in 4 sets, 2 for each breed: underfed group fed on wheat straw (restricted diet, so their body weight would be 15–20% reduced by the end of experiment), and a control group fed with an energy-balanced diet. At the end of the experimental period (22 days), mammary gland biopsies were obtained for all experimental groups. The proteomic analysis of the mitochondria enabled the resolution of a total of 277 proteins, and 148 (53%) were identified by MALDI-TOF/TOF mass spectrometry. Some of the proteins were identified as subunits of the glutamate dehydrogenase complex and the respiratory complexes I, II, IV, V from mitochondria, as well as numerous other proteins with functions in: metabolism, development, localization, cellular organization and biogenesis, biological regulation, response to stimulus, among others, that were mapped in both BN and 2DE gels. The comparative proteomics analysis enabled the identification of several proteins: NADH-ubiquinone oxidoreductase 75 kDa subunit and lamin B1 mitochondrial (up-regulated in the Palmera breed), Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 (up-regulated in the Majorera breed) and cytochrome b-c1 complex subunit 1, mitochondrial and Chain D, Bovine F1-C8 Sub-Complex Of Atp Synthase (down-regulated in the Majorera breed) as a consequence of weight loss.

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“…Although the transfer of immunoglobulins (IgG and IgM, mainly) is a key factor for host defense against pathogens, a wider range of colostrum components, such as the complement system proteins, have been suggested to contribute to the early protection of the neonate (Smith and Foster, 2007;Hernández-Castellano et al, 2015a). The complement system activity -comprising the total (TCA) and alternative (ACA) pathways -plays an important role in host defense mechanisms against infectious microbes, because it is involved in specific and non-specific immunity (Rodríguez et al, 2009;Hernández-Castellano et al, 2015b).…”

Section: Introductionmentioning

confidence: 99%

“…No references about the amount of colostrum required by newborn lambs reared under artificial conditions have been found. As it is essential to obtain adequate immunoglobulin concentrations in the blood (O'Doherty and Crosby, 1997;Quigley et al, 2000;Christley et al, 2003), this knowledge has high relevance for the survival of the lambs.Although the transfer of immunoglobulins (IgG and IgM, mainly) is a key factor for host defense against pathogens, a wider range of colostrum components, such as the complement system proteins, have been suggested to contribute to the early protection of the neonate (Smith and Foster, 2007;Hernández-Castellano et al, 2015a). The complement system activity -comprising the total (TCA) and alternative (ACA) pathways -plays an important role in host defense mechanisms against infectious microbes, because it is involved in specific and non-specific immunity (Rodríguez et al, 2009;Hernández-Castellano et al, 2015b).…”

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confidence: 99%

The effect of colostrum period management on BW and immune system in lambs: from birth to weaning

Hernández-Castellano

Suárez-Trujillo

Martell-Jaizme

et al. 2015

Animal

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The aim of this study was to investigate the BW and immune status of lambs reared under natural conditions or under artificial conditions fed two different colostrum amounts. In this study, 60 lambs were randomly divided into groups according to treatment. Twenty lambs remained with their dams (natural rearing (NR) group). Forty lambs were removed from their dams at birth. Lambs were bottle-fed with a pool of sheep colostrum, receiving either 4 g of IgG/kg of BW at birth (C4 group) or 8 g of IgG/kg of BW at birth (C8 group). The total colostrum amount was equally divided into three meals at 2, 14 and 24 h after birth. After this period, lambs were bottle-fed a commercial milk replacer. Blood plasma sample analysis and BW recordings were carried out before feeding at birth and then at 1, 2, 3, 4, 5 and 20 days after birth. Another blood sample analysis and BW recording was carried out when animals reached 10 kg of BW. During weaning (30 days), sampling was carried out every 5 days. Blood plasma was used to determine the concentrations of IgG and IgM and the complement system activity -total and alternative pathways. The NR group showed greater BW than the C4 and C8 groups during milk feeding period, whereas the C4 and C8 groups had greater BW than the NR group at the end of weaning period. The C8 and NR groups had greater plasma IgG and IgM concentrations than the C4 group during milk feeding period. In addition, C4 and C8 groups showed similar IgG concentrations and greater IgM concentrations than the NR group at the end of the weaning period. Complement system activity was greater in the NR group than in the C4 and C8 groups during the first 3 days after birth. In conclusion, lambs fed amounts of colostrum equivalent to 8 g of IgG/kg of BW showed similar immune variables compared to lambs reared under natural conditions, obtaining a greater BW at the end of the weaning period. Nevertheless, this study shows that not only the colostrum amount but also the management during the milk feeding and weaning period, such as stress produced by dam separation, milk quality and suckling frequency, can affect the final immune status of lambs.Keywords: immune, lamb, colostrum, weaning, artificial rearing ImplicationsColostrum plays a fundamental role in the immunization of newborn ruminants. Artificial rearing is a common method used in dairy farms, increasing the amount of milk for processing. The aim of this study was to feed newborn lambs colostrum with two different IgG concentrations and to compare these animals to natural dam fed lambs. This study showed that administration of 8 g of immunoglobulin g/kg BW at birth, equally divided into three colostrum meals (2, 14 and 26 h), achieved higher circulating immunoglobulin G concentration when compared to the natural rearing treatment, although both artificial rearing groups decreased BW after birth. IntroductionThe relationship between colostrum and the survival of newborn ruminants has been long characterized (Argüello et al., 2004;Castro et al., 2011;Abdou et al., 2014)...

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Colostrum protein uptake in neonatal lambs examined by descriptive and quantitative liquid chromatography-tandem mass spectrometry

Cited by 42 publications

References 74 publications

Effect of Rearing System on the Straight and Branched Fatty Acids of Goat Milk and Meat of Suckling Kids

Effect of Rearing System on the Straight and Branched Fatty Acids of Goat Milk and Meat of Suckling Kids

The Goat (Capra hircus) Mammary Gland Mitochondrial Proteome: A Study on the Effect of Weight Loss Using Blue-Native PAGE and Two-Dimensional Gel Electrophoresis

The effect of colostrum period management on BW and immune system in lambs: from birth to weaning

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