Colorimetric visualization of histamine secreted by basophils based on DSP-functionalized gold nanoparticles

Ling, Xiaojing; Liu, Yongxin; Zhu, Dan-Xuan; An, Weizhen; Geng, Jiaying; Li, Lin; Wei, Ji‐Fu

doi:10.1039/d2ay00379a

Anal. Methods

2022

DOI: 10.1039/d2ay00379a

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Colorimetric visualization of histamine secreted by basophils based on DSP-functionalized gold nanoparticles

Xiaojing Ling

Yongxin Liu

Dan-Xuan Zhu

et al.

Abstract: In this study, we developed a novel nanosensor, for the first time, to visually detect endogenous histamine changes in activated basophils by simply coupling dithiobis(succinimidylpropionate) on the surface of AuNPs.

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Label‐Free Fluorescence Turn‐On Detection of Histidine‐Tagged Proteins Based on Intramolecular Rigidification Induced Emission

Huang

et al. 2023

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Histidine tag (His-tag) is a representative epitope tag, which has been widely used in the purification or immobilization of recombinant proteins. In this study, a novel probe CTPE-IDA-Ni 2 + based on intramolecular rigidification induced emission for fluorescence detecting His-tagged proteins is successfully developed and experimentally demonstrated. This detecting system is based on TPE derivative incorporated IDA-Ni 2 + moiety for real-time detection of His-tagged proteins. In the presence of His-tagged proteins, the IDA-Ni 2 + -His-tag complex was formed, which restricted the rotation of two phenyl rings linked with TPE and subsequently resulting in the fluorescence turn on. Results have shown that our probe could directly detect His-tagged proteins with good selectivity and fast response within 10 min. In addition, we have proved that this system could be used in fluorescent labeling of His-tagged protein instead of traditional Western blot assay with simple procedure, high quality and low background interference, as well as without aggregation-caused quenching (ACQ) problems like some organic dyes.

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Label‐Free Fluorescence Turn‐On Detection of Histidine‐Tagged Proteins Based on Intramolecular Rigidification Induced Emission

Huang

et al. 2023

ChemistrySelect

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show abstract

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Colorimetric visualization of histamine secreted by basophils based on DSP-functionalized gold nanoparticles

Abstract: In this study, we developed a novel nanosensor, for the first time, to visually detect endogenous histamine changes in activated basophils by simply coupling dithiobis(succinimidylpropionate) on the surface of AuNPs.

Cited by 1 publication

References 30 publications

Label‐Free Fluorescence Turn‐On Detection of Histidine‐Tagged Proteins Based on Intramolecular Rigidification Induced Emission

Label‐Free Fluorescence Turn‐On Detection of Histidine‐Tagged Proteins Based on Intramolecular Rigidification Induced Emission

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